Abstract
The serological relationships of Potato Virus Y (PVY) isolates belonging to the pepper pathotypes 0, 1 and 1-2 were established by enzyme-linked immunosorbent assay (ELISA). PVY pepper pathotypes did not react with monoclonal antibodies which typically recognize non-pepper strains within the PVY group, leading to discrimination between these two groups of strains. No serological differences were found between the three PVY pepper pathotypes. The coat protein (CP) nucleotide and predicted amino acid sequences of the three different PVY pepper pathotypes were determined. The highest sequence similarity was found between pathotypes 0 and 1 (99.2%), while the lowest occurred between these two and pathotype 1–2 (98.1%). PVY strains from potato and tobacco appeared more distantly related. Phenetic analysis of the CP amino acid sequences showed that the PVY pepper pathotypes formed a tightly clustered group separate from other PVY strains.
Similar content being viewed by others
References
Arroyo R, Soto MJ, Martínez-Zapater JM and Ponz F (1996) Impaired cell-to-cell movement of potato virus Y in pepper plants carrying the y a (pr2 1) resistance gene. Mol Plant-Microbe Interact 9: 314-318
Berzal-Herranz A, De la Cruz A, Tenllado F, Díaz-Ruíz JR, López L, Sanz AI, Vaquero C, Serra MT and García-Luque I (1995) The Capsicum L3 gene-mediated resistance against the tobamoviruses is elicited by the coat protein. Virology 209: 498-505
Blanco-Urgoiti B, Sánchez P, Pérez de San Román C, Dopazo J and Ponz, F (1998a) Potato virus Y group C isolates are a homogeneous pathotype but two different genetic strains. J Gen Virol 79: 2037-2042
Blanco-Urgoiti B, Tribodet M, Leclere S, Ponz F, Pérez de San Román C, Legorburu FJ and Kerlan C (1998b) Characterization of potato potyvirus Y (PVY) isolates fron seed potato batches. Situation of the NTN, Wilga and Z isolates. Eur J Plant Pathol 104: 811-819
Bravo-Almonacid FF and Mentaberry AN (1989) Nucleotide cDNA coding for the PVY o coat protein. Nucleic Acids Res 17: 4401
Canto T, López-Moya JJ, Serra-Yoldi MT, Díaz-Ruíz JR and López-Abella D (1995) Different helper component mutations associated with lack of aphid transmissibility in two isolates of potato virus Y. Phytopathology 85: 1519-1524
Chachulska AM, Chrzanowska M, Robaglia C and Zagórski W (1997) Tobacco veinal necrosis determinants are unlikely to be located within the 5′ and 3′ terminal sequences of the potato virus Y genome. Arch Virol 142: 765-779
Converse RH and Martin RR (1991) Methods for plant viruses. In: Hampton R, Ball E and De Boer S (eds) Serological Methods for Detection and Identification of Viral, Bacterial and Plant Pathogens, APS Press, St. Paul, Minnesota
De Bokx JA and Huttinga H (1981) Potato Virus Y. CMI/AAB Descriptions of Plant Viruses, no 242
De la Cruz A, López L, Tenllado F, Díaz-Ruíz JR, Sanz AI, Vaquero C, Serra MT and García-Luque I (1997) The coat protein is required for the elicitation of the Capsicum L2 genemediated resistance against the tobamoviruses. Mol Plant-Microbe Interact 10: 107-113
Dolja VV, Haldeman R, Robertson NL, Dougherty WG and Carrington JC (1994) Distinct functions of capsid protein in assembly and movement of tobacco etch potyvirus in plants. EMBO J 12: 1482-1491
Dolja VV, Haldeman-Cahill R, Montgomery AE, Vandenbosch KA and Carrington JC (1995) Capsid protein determinants involved in cell to cell and long distance movement of tobacco etch potyvirus. Virology 206: 1007-1016
Drake JW (1993) Rates of spontaneous mutations among RNA viruses. Proc. Natl. Acad. Sci. USA 90: 4171-4175
Gebre-Selassie K, Marchoux G, Delecolle B and Pochard E (1985) Variabilite, naturelle des souches du virus Y de la pomme de terre dans les cultures de piment du sud-est de la France. Caracterisation et classification en pathotypes. Agronomie 5: 621-630
Gilardi P, García-Luque I and Serra MT (1998) Pepper mild mottle virus coat protein alone can elicit the Capsicum spp. L3 gene-mediated resistance. Mol Plant-Microbe Interact 11: 1253-1257
Gugerli P and Fries P (1983) Characterization of monoclonal antibodies to potato virus Y and their use for virus detection. J Gen Virol 64: 2471-2477
Hay JM, Fellowes AP and Timmerman GM (1989) Nucleotide sequence of the coat protein gene of a necrotic strain of potato virus Y from New Zealand. Arch Virol 107: 111-122
Hidaka M, Yoshida Y, Masaki H, Namba S, Yamashita S, Tsuchizaki T and Uozumi T (1992) Cloning and sequencing of the 3′ half of a potato virus Y (0 strain) genome encoding 5K protein, protease, polymerase, and coat protein. Nucleic Acids Res 20: 3515
Jordan R and Hammond J (1991) Comparison and differentiation of potyvirus isolates and identification of strain-, virus-, subgroup-specific and potyvirus group-common epitopes using monoclonal antibodies. J Gen Virol 72: 25-36
Logemann J, Schell J and Willmitzer L (1987) Improved method for the isolation of RNA from plant tissues. Anal Biochem 163: 16-20
Luis-Arteaga M (1989) Virosis y micoplasmosis del pimiento cultivado al aire libre en España. Identificación de virus y caracterización de cepas. Ph.D. Thesis, ETSIA, Madrid, Spain
McKern NM, Strike PM, Bernett OW, Dijkstra J, Shukla DD and Ward CW (1994) Cowpea aphid borne mosaic virus-Morocco and South African Passiflora virus are strains of the same potyvirus. Arch Virol 136: 207-217
Murphy JF, Rhoads RE, Hunt AG and Shaw JG (1990) The VPg of tobacco etch virus RNA is the 49-kDa proteinase of the N-terminal 24-kDa part of the proteinase. Virology 178: 285-288
Murphy FA, Fauquet CM, Bishop DHL, Gabrial SA, Jarvis AW, Martelli GP, Mayo MA and Summers MD (1995) Virus taxonomy Arch Virol [Suppl.] 10: 586
Palloix A and Kyle MM (1995) Proposed revision of gene nomenclature for potyvirus resistance genes in Capsicum sp. Capsicum Eggplant Newsl 14: 26-29
Pasko P (1993) A study on pepper resistance to potato virus Y (PVY). M.S. Thesis. IAMZ, Zaragoza, Spain
Pirone TP (1991) Viral genes and gene products that determine insect transmissibility. Sem Virol 2: 81-87
Pirone TP and Blanc S (1996) Helper-dependent vector transmission of plant viruses. Annu Rev Phytopathol 34: 227-247
Riechmann JL, Laín S and García JA (1992) Highlights and prospects of Potyvirus molecular biology. J Gen Virol 73: 1-16
Robaglia C, Durand-Tardif M, Tronchet M, Boudazin G, Astier-Manifacier S and Casse-Delbart F (1989) Nucleotide sequence of potato virus Y (N strain) genomic RNA. J Gen Virol 70: 935-947
Sanz A, Cambra M, Pérez de San Román C, Miguet JG, Cortés E, Gorris MT and Vela C (1990) Preparation of additional monoclonal antibodies for detection and discrimination of potato virus Y isolates infecting potato. Potato Res 33: 365-375
Sambrook J, Fritsch EF and Maniatis T (1989) Molecular cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York
Shukla DD and Ward CW (1988) Aminoacid sequence homology of coat protein as a basis for identification and classification of the potyvirus group. J Gen Virol 69: 2703-2710
Shukla DD, Strike PM, Tracy SL, Gough KH and Ward CW (1988a) The N and C termini of the coat protein of potyviruses are surface-located and the N terminus contains the major virus-specific epitopes. J Gen Virol 69: 1497-1508
Shukla DD, Thomas JE, McKern NM, Tracy SL and Ward CW (1988b) Coat protein of potyviruses 4. Comparison of biological properties serological relationships, and coat protein aminoacid sequences of four strains of potato virus Y. Arch Virol 102: 207-219
Shukla DD, Lauricella R and Ward CW (1992) Serology of potyviruses: current problems and some solutions. In: Barnett OW (ed) Potyvirus Taxonomy (pp 57-69). Springer, Wien, New York (Arch Virol [Suppl] 5)
Soto MJ, Luis-Arteaga M, Fereres, A and Ponz F (1994) Limited degree of serological variability in pepper strains of potato virus Y as revealed by analysis with monoclonal antibodies. Ann appl Biol 124: 37-43
Sudarsono S, Woloshuk L, Xiong Z, Hellamnn GM, Wernsman EA, Weissinger AK and Lommel SA (1993) Nucleotide sequence of the capsid protein cistrons from six potato virus Y (PVY) isolates infecting tobacco. Arch Virol 132: 161-170
Suranto K, Gough G, Shukla DD and Pallaghy CK (1998) Coat protein sequence of Krish-infecting strain of Johnsongrass mosaic potyvirus. Arch Virol 143: 1015-1020
Thompsom JD, Higgins DG and Gibson TJ (1994) CLUSTAL W: improving the sensivity of progressive multiple sequence alignment though sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673-4680
Ullman DE, German TL, Sherwood JL, Westcot DM and Cantone FA (1993) Tosposvirus replication in insect vector cells: immunocytochemical evidence that the nonstructural protein encoded by the sRNA of tomato spotted wilt tospovirus is present in thrips vector cells. Phytopathology 83: 456-463
Valkonen JPT, Puurand Ü, Slack SA, Mäkinen K and Saarma M (1995) Identification of three strain groups of potato virus A based on hypersensitive responses in potato, serological properties, and coat protein sequences. Plant Dis 79: 748-753
Van der Vlugt RAA, Leunissen J and Goldbach R (1993) Taxonomic relationships between distinct potato virus Y isolates based on detailed comparisons of the viral coat proteins and 3′-nontranslated regions. Arch Virol 131: 361-375
Vance VB, Moore D, Turpen TH, Bracker A and Hollowell VC (1992) The complete nucleotide sequence of pepper mottle virus genomic RNA: Comparison of the encoded polyprotein with those of other sequenced potyviruses. Virology 191: 19-30
Ward CW, McKern NM, Frenkel MJ and Shukla DD (1992) Sequence data as the major criterion for potyvirus classification. In: Barnett OW (ed) Potyvirus Taxonomy (pp 283-297). Springer, Wien, New York (Arch Virol [Suppl] 5)
Xiao XW, Frenkel MJ, Teakle DS, Ward CW and Shukla DD (1993) Sequence diversity in the surface-exposed aminoterminal region of the coat proteins of seven strains of sugarcane mosaic virus correlates with their host range. Arch Virol 132: 399-408
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Llave, C., Martínez, B., Díaz-Ruíz, J. et al. Serological Analysis and Coat Protein Sequence Determination of Potato Virus Y (PVY) Pepper Pathotypes and Differentiation from Other PVY Strains. European Journal of Plant Pathology 105, 847–857 (1999). https://doi.org/10.1023/A:1008775910680
Issue Date:
DOI: https://doi.org/10.1023/A:1008775910680