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A method for estabilishing primary cultures of human gastric epithelial cells

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Methods in Cell Science

Abstract

Long-term culture of human gastric epithelial cells has been difficult, and at present no normal human gastric epithelial cell lines are readily available. As part of our experiments to study pathogenesis of H. pylori, a bacterium that infects the stomach, we developed methods to culture normal human gastric epithelial cells. Primary cultures of human gastric epithelial cells can be established from gastric biopsies taken at upper G.I. endoscopy. Enzymatically isolated gastric epithelial-like cells are present in tight colonies on culture dishes within 24 hours of placing the cells in culture. Cells isolated stain positively for cytokeratin and produce neutral mucins, indicating that they are mucin secreting epithelial cells, consistent with gastric epithelial cells. Epithelial cells can be maintained up to 4 weeks in culture with evidence of DNA synthesis up through the first week of culture.

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References

  1. Tokiwa T, Lipsky MM, Smoot DT, et al. (1994). A method for transforming human liver epithelial cells by transfection using a plasmid containing SV 50 early region gene. Journal of Tissue Culture Methods 16: 57-60.

    Google Scholar 

  2. Hsu IC, Lipsky MM, Cole KE, et al. (1985). Isolation and culture of hepatocytes from human liver of immediate autopsy. In Vitro Cell and Developmental Biology 21: 154-160.

    Google Scholar 

  3. Smoot DT, Resau JH, Naab T, et al. (1993). Adherence of Helicobacter pylori to cultured human gastric epithelial cells. Infect Immun 61: 350-355.

    Google Scholar 

  4. Smoot DT, Resau JH, Earlington MH, et al. (1996). Effects of Helicobacter pylori vaculoating cytotoxin on primary cultures of human gastric epithelial cells. Gut 39: 795-799.

    Google Scholar 

  5. Smoot DT, Earlington MH, Abebe E, et al. (1996). Effects of sucralfate on vacuolating cytotoxin activity and adherence of Helicobacter pylori to human gastric epithelial cells. J Assoc Acad Minor Phys 7: 88-92.

    Google Scholar 

  6. Boland CR, Kraus ER, Scheiman JM, et al. (1990). Characterization of mucous cell synthetic functions in a new primary canine gastric mucous cell culture system. Am J Physiol 258: G774-G784.

    Google Scholar 

  7. Evans DG, Evans DJ, Moulds JJ, et al. (1988). Nacetylneuraminyllactose-binding fibrillar hemagglutinin of Campylobacter pylori: A putative colonization factor antigen. Infect Immun 56: 2896-2906.

    Google Scholar 

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Authors
  1. Duane T. Smoot
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  2. Joel Sewchand
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  3. Karen Young
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  4. Byron C. Desbordes
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  5. Cornell R. Allen
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  6. Tammy Naab
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Smoot, D.T., Sewchand, J., Young, K. et al. A method for estabilishing primary cultures of human gastric epithelial cells. Methods Cell Sci 22, 133–136 (2000). https://doi.org/10.1023/A:1009846624044

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  • DOI: https://doi.org/10.1023/A:1009846624044

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