Abstract
Aphthoviruses are unique among picornaviruses in that they alone encode a functional L proteinase as the first component of the viral polyprotein. The L genes of a few Indian foot-and-mouth disease viruses were sequenced and compared with those available to study the extent of variation in this gene. Besides the two in-frame start codons present in all FMDV L genes, the Asia-I vaccine virus had an additional in-frame AUG (start) codon, at codon position 3. Amino acid sequence comparison revealed that 39.8% of positions were capable of accepting replacements, yet the residues of the catalytic dyad were totally conserved. Sequence comparison at the C-terminus of the protein indicated that K/R↓GAGQS is sufficient for L/P1 cleavage. Phylogenetic analysis based on the L gene sequences did not reveal any serotype-specific clustering. The probable implications of the observed high variability in this non-structural gene is briefly discussed.
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Sanger D.V., Newton S.E., Rowlands D.J., and Clarke B.E., Nucl Acids Res 15, 3305–3315, 1987.
Belsham G.J., EMBO J 11, 1105–1110, 1992.
Medina M., Domingo E., Brangwyn J.K., and Belsham G.J., Virology 194, 355–359, 1993.
Gorbalenya A.E., Koonin E.V., and Lai M.M., FEBS Lett 288, 201–205, 1991.
Strebel K. and Beck E., J Virol 58, 893–899, 1986.
Devaney M.A., Vakharia V.N., Lloyd R.E., Ehrenfeld E., and Grubman M.J., J Virol 62, 4407–4409, 1988.
Kirchweger R., Ziegler E., Lamphear B.J., Waters D., Liebig H.D., Sommergruber W., Sobrino F., Hohenadl C., Blass D., Rhoads R.E., and Skern T., J Virol 68, 5677–5684, 1994.
Domingo E. and Holland J.J., in Morse, S.S (ed.) Evolutionary Biology of Viruses. Raven Press, New York, 1994, pp. 161–184.
Eigen M. and Biebricher C.K., in Domingo E., Ahlquist P., and Holland J.J. (eds) RNA Genetics: Variability of RNA Genomes Vol. 3, CRC Press, Boca Raton, FL, 1988, pp. 211–245.
Novella I.S., Elena S.F., Moya A., Domingo E., and Holland J.J., J Virol 69, 2869–2872, 1995.
Escarmis C., Davila M., Charpentier N., Bracho A., Moya A., and Domingo E., J Mol Biol 264, 255–267, 1996.
Charpentier N., Davila M., Domingo E., and Escarmis C., Virology 223, 10–18, 1996.
Roberts P.J. and Belsham G.J., Virology 213, 140–146, 1995.
Forss S., Strebel K., Beck E., and Schaller H., Nucl Acids Res 12 (16), 6587–6601, 1984.
Felsenstein J., PHYLIP Version 3.5c. Department of Genetics, University of Washington, Seattle, 1995.
Lopez de Quinto S. and Martinez-Salas E., Virology 255, 324–336, 1999.
Piccone M.E., Zellner M., Kumosinski T.F., Mason P.W., and Grubman M.J., J Virol 69, 4960–4956, 1995.
Skern T., Fita I., and Guarne A., J Gen Virol 79, 301–307, 1998.
Piccone M.E., Rieder E., Mason P.W., and Grubman M.J., J Virol 69, 5376–5382, 1995.
Domingo E. and Holland J.J., Ann Rev Microbiol 51, 151–178, 1997.
Etchison D., Milburn S.C., Edery I., Sonenberg N., and Hershey J.W.B., J Biol Chem 257, 14806–14810, 1982.
Wimmer E., Hellen C.U.T., and Cao X., Ann Rev Gen 27, 353–436, 1993.
de la Torre J.C., Davila M., Sobrino F., Ortin J., and Domingo E., Virology 145, 24–35, 1985.
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George, M., Venkataramanan, R., Gurumurthy, C. et al. The Non-structural Leader Protein Gene of Foot-and-Mouth Disease Virus is Highly Variable between Serotypes. Virus Genes 22, 271–278 (2001). https://doi.org/10.1023/A:1011153904910
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DOI: https://doi.org/10.1023/A:1011153904910