Abstract
Cytotoxicity of Ni ions on three fibroblasts such as L929, Balb/3T3 clone A31 and MC3T3–E1 were examined by cell count (CC) and Neutral Red assay (NR). Three cells were incubated for 6 days in 1 ml DME medium containing Ni ions which ranged from 0 to 2 mM/l. The results clarified that Ni ions had dose-dependent cytotoxicity. L929 possessed the largest TC50 values (the amount of Ni ion that caused 50% cell death or 50% cell viability) of 0.12 mM/l (CC) and 0.32 mM/l (NR), and Balb/3T3 clone A31 had the least values of 0.05 mM/l (CC) and 0.09 mM/l (NR), whilst MC3T3–E1 had the intermediate values of 0.08 mM/l (CC) and 0.15 mM/l (NR). The dissolution of Ni ions from Ni-containing metallic restorations must be lower than these concentration levels so that body tissues might not be severely damaged. © 2001 Kluwer Academic Publishers
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Taira, M., Toguchi, M.S., Hamada, Y. et al. Studies on cytotoxic effect of nickel ions on three cultured fibroblasts. Journal of Materials Science: Materials in Medicine 12, 373–376 (2001). https://doi.org/10.1023/A:1011295115612
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DOI: https://doi.org/10.1023/A:1011295115612