Abstract
Galectin-3 (formerly called Mac-2 antigen) is a ∼30 kDa carbohydrate-binding protein expressed on the surface of inflammatory macrophages and several macrophage cell lines. We have purified from lysates of the murine macrophage cell line WEHI-3 glycoproteins that bind to a galectin-3 affinity column. Several of these receptors are labelled after biotinylation of intact cells showing their location at the cell surface. N-terminal aminoacid sequencing of intact galectin-3-binding glycoproteins isolated from preparative SDS-gels or of chemically derived fragments showed several homologies with known proteins and identification was confirmed by immunoprecipitation with specific antibodies. The glycoproteins were shown to be: the α-subunit(CD11b) of the CD11b/CD18 integrin(Mac-1 antigen); the lysosomal membrane glycoproteins LAMPs 1 and 2 which are known in part to be expressed at cell surfaces; the Mac-3 antigen, a mouse macrophage differentiation antigen defined by the M3/84 monoclonal antibody and related immunochemically to LAMP-2; the heavy chain of CD98, a 125 kDa heterodimeric glycoprotein identified by the 4F2/RL388 monoclonal antibodies respectively on human and mouse monocytes/macrophages and on activated T cells. Further studies showed that CD11b/CD18, CD98 and Mac-3 are major surface receptors for galectin-3 on murine peritoneal macrophages elicited by thioglycollate. Abbreviations: PBS, phosphate buffered saline; CNBR, cyanogen bromide; PMSF, phenyl methyl sulphonyl fluoride
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Barondes SH, Cooper DNW, Gitt MA, Leffer H (1994) J Biol Chem 269: 20807-10.
Hughes RC (1994) Glycobiol. 4: 5-12.
Sato S, Burdett IDJ, Hughes RC (1993) Exp Cell Res 207: 8-18.
Springer TA (1981) J Biol Chem 256: 3833-8.
Ho M-K, Springer T (1982) J Immunol 128: 1221-8.
Leenen PJM, de Bruijn FTR, Voerman JSA, Campbell PA, van Ewijk W (1994) J Immunol Methods 174: 5-19.
Ralph P, Ho M-K, Litcofsky PB, Springer TA (1983) J Immunol 130: 108-14.
Leenen PJM, Jansen AMAC, van Ewijk W (1986) Differentiation 32 157-64.
Sato S, Hughes RC (1994) Eur J Immunol 24: 216-21.
Sato S, Hughes RC (1994) J Biol Chem 269: 4424-30.
Sato S, Hughes RC (1992) J Biol Chem 267: 6983-90.
Jeng KC, Frigeri LG, Liu F-T (1994) Immunol Letters 42: 113-16.
Yamaoka A, Kuwabara I, Frogeri LG, Liu F-T (1995) J Immunol 154: 3479-87.
Zuberi RI, Frigeri LG, Liu F-T (1994) Cell Immunol 156: 1-12.
Frigeri LG, Zuberi RI, Liu F-T (1993) Biochemistry 32: 7644-9.
Truong M-T, Gruart JP, Kusnierz J-P, Papin S, Loiseau S, Capron A, Capron M (1993) J Exp Med 177: 243-8.
Probstmeier R, Montag D, Schachner M (1995) J Neurochem 64: 2465-72.
Rosenberg I, Cherayil BJ, Isselbacher KJ, Pillai S, (1991) J Biol Chem 266: 18731-6.
Inohara H, Raz A (1994) Biochem Biophys Res Commun 201: 1366-75.
Koths K, Taylor E, Haenbeck R, Casipit C, Wang A (1993) J Biol Chem 268: 14245-9.
Chicheportiche Y, Vassalli P (1994) J Biol Chem 269: 5512-17.
Vlassara H, Li YM, Imani F, Wojciechowicz D, Yang Z, Liu F-T, Cerami A (1995) Nature Molec Med 1: 634-46.
Ho M-K, Springer T (1983) J Biol Chem 258: 636-42.
Mehul B, Bawumia S, Martin SR, Hughes RC (1994) J Biol Chem 269: 18250-8.
Schagger H, VonJagow G (1987) Anal Biochem 166: 368-79.
Springer TA, Galfre G, Secher DS, Milstein C (1979) Eur J Immunol 9: 301-6.
Arnaout MA (1990) Blood 75: 1037-50.
Diamond MS, Staunton DE, Marlin SD, Springer TA (1991) Cell 65: 961-71.
Altieri DC, Stamnes SF, Gahmberg CG, (1992) Biochem J 288: 465-73.
Asada M, Furukawa K, Kantor C, Gahmberg C, Kobata A (1991) Biochemistry 30: 1561-71.
Chen JW, Murphy TL, Willingham MC, Pastan I, August JT, (1985) J Cell Biol 101: 85-95
Hemler ME, Strominger JL (1982) J Immunol 129: 623-8.
MacDonald HR, Lees RK, Bron C (1985) J Immunol 135: 3944-50
Luscher B, Pousseaux M, Lees R, MacDonald HR, Bron C (1986) J Immunol 135, 3951-7.
Bron C, Rousseaux M, Spiazzi A-L, Macdonald HR (1986) J Immunol 137, 397-9.
Parmacek MS, Karpinski BR, Gottesdiener KM, Thompson CB, Leiden JM (1989) Nucleic Acids Res 17: 1915-32.
Michalak M, Quackenbush EJ, Letarte M (1986) J Biol Chem 261, 92-5.
Messika EJ, Avni O, Gallily R, Yefenof E, Baniyash M (1995) J Immunol 154: 6563-70.
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Dong, S., Colin Hughes, R. Macrophage surface glycoproteins binding to galectin-3 (Mac-2-antigen). Glycoconj J 14, 267–274 (1997). https://doi.org/10.1023/A:1018554124545
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DOI: https://doi.org/10.1023/A:1018554124545