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Adsorption of Oligonucleotides onto Polyisohexylcyanoacrylate Nanoparticles Protects Them Against Nucleases and Increases Their Cellular Uptake

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Abstract

Oligonucleotides can be adsorbed on polyisohexylcyanoacrylate nanoparticles in the presence of hydrophobic quartenary ammonium salts. Oligonucleotides bound to nanoparticles are protected from nuclease attack both in buffer and in cell culture media. Cellular uptake of Oligonucleotides is increased when they are adsorbed onto nanoparticles as a result of the capture of nanoparticles by an endocytic/phagocytic pathway. Intracellular stability towards nucleolytic degradation is increased in the presence of nanoparticles. These results show that nanoparticles can be considered as convenient carriers for the protection and delivery of oligonucleotides to cells.

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Authors and Affiliations

  1. Laboratoire de Biophysique, Muséum National d’Histoire Naturelle, INSERM U 201, CNRS URA 481, 43 rue Cuvier, 75231, Paris cedex 05, France

    Christine Chavany, Tula Saison-Behmoaras & Claude Hélène

  2. Laboratoire de Chimie, Muséum National d’Histoire Naturelle, 63 rue Buffon, 75005, Paris, France

    Trung Le Doan

  3. Laboratoire de Physico-Chimie des Surfaces et Innovation en Pharmacotechnie, Université de Paris-Sud, CNRS URA 1218, 92296, Chatenay-Malabry Cedex, France

    Christine Chavany, Francis Puisieux & Patrick Couvreur

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  1. Christine Chavany
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  2. Tula Saison-Behmoaras
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  3. Trung Le Doan
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  4. Francis Puisieux
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  5. Patrick Couvreur
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  6. Claude Hélène
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Chavany, C., Saison-Behmoaras, T., Doan, T.L. et al. Adsorption of Oligonucleotides onto Polyisohexylcyanoacrylate Nanoparticles Protects Them Against Nucleases and Increases Their Cellular Uptake. Pharm Res 11, 1370–1378 (1994). https://doi.org/10.1023/A:1018923301967

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