Abstract
A strain of infectious bursal disease virus (IBDV) was detected from bursal tissues of chicks which suffered from infectious bursal disease (IBD) in Chinju, Korea and provisionally named as Chinju strain. A full-length cDNA clone for segment A gene of the virus was constructed, and complete nucleotide sequence of the gene including noncoding region was determined and analyzed by comparison with that of other IBDV strains. The segment A gene of Chinju strain consisted of 3,269 nucleotides including 862 adenine (26.4%), 917 cytosine (28.0%), 854 guanine (26.1%) and 636 thymine (19.5%). There were regions for two open reading frames (ORFs), ORF1 encoding the VP5 with ATG codon at nucleotides 98–100 and ORF2 encoding the polyprotein of VP2, VP4 and VP3 in the nucleotides 132–3,170. In deduced translation the ORF2 encoded 1,012 amino acids. The full nucleotide sequence of segment A gene and amino acid sequence of ORF2 of the Chinju strain showed 98–99% homology with those of the very virulent IBDVs (vvIBDVs) such as HK46, OKYM, UK661, UPM97/61, D6948 and BD3/99. In phylogenetic analysis of nucleotide and amino acid sequences, the Chinju strain was also related closely to the vvIBDVs. Hence, it was suggested that the Chinju strain is a vvIBDV. The nucleotide and amino acid sequences of the Chinju strain with pertinent information can be useful for the development of genetically engineered vaccines and diagnostic reagents against vvIBDV.
Similar content being viewed by others
References
Cosgrove A.S., Avian Dis 6, 385-389, 1962.
Burkhardt E. and Muller H., Arch Virol 94(3–4), 297-303, 1987.
Kibenge F.S.B., Dhillon A.S., and Russell R.G., J Gen Virol 69, 1757-1775, 1988.
Azad A.A., Barrett S.A., and Fahey K.J., Virology 143, 35-44, 1985.
Dobos P., Hill B.J., Hallett R., Kells D.T., Becht H., and Teninges D., J Virol 32, 593-605, 1979.
Spies U., Muller H., and Becht H., Virus Res 8, 127-140, 1987.
Mundt E., Beyer J., and Muller H., J Gen Virol 76, 437-443, 1995.
Mundt E., Kollner B., and Kretzschmar D., J Virol 71, 5647-5651, 1997.
Fahey K.J., O'Donnell I.J., and Azad A.A., J Gen Virol 66, 1479-1488, 1985.
Spies U., Muller H., and Becht H., Nucleic Acids Res 17, 7982, 1989.
Heine H.G., Haritou M., Failla P., Fahey K., and Azad A.A., J Gen Virol 72, 1835-1843, 1991.
Jagadish M.N. and Azad A.A., Virology 184, 805-807, 1991.
Mahardika G.N.K. and Becht H., Arch Virol 140, 765-774, 1995.
McFerran J.B., McNulty M.S., Killop E.R., Conner T.J., McCracken R.M., Collins P.S., and Allan G.M., Avian Pathol 9, 395-404, 1980.
Ismail N.M., Saif Y.M., and Moorhead P.D., Avian Dis 32, 757-759, 1988.
Lukert P.D. and Saif Y.M., in Calnek B.W., Barnes H.J., Beard C.W., McDougald L.R. and Saif Y.M. (eds.), Diseases of Poultry, 10th edn. Iowa State University Press, Ames, 1997, pp. 721-738.
Sharma J.M., Dohms J.E., and Metz A.L., Avian Dis 33, 112-124, 1989.
Chettle N.J., Stuart J.C., and Wyeth P.J., Vet Rec 125, 271-272, 1989.
Eterradossi N., Picault J.P., Drouin P., Guittet M., L'Hospitalier R., and Bennejean G., Zentralbl Veterinaemed 39, 683-691, 1992.
Oppling V., Muller H., and Becht H., Arch Virol 119, 211-223, 1991.
van den Berg T.P., Gonze M., and Meulemans G., Avian Pathol 20, 133-143, 1991.
Lin Z., Kato A., Otaki Y., Nakamura T., Sasmaz E., and Ueda S., Avian Dis 37, 315-323, 1993.
Tsai H.J. and Lu Y.S., J Chin Soc Vet Sci 19, 249-258, 1993.
Lim B.L., Cao Y., Yu T., and Mo C.W., J Virol 73, 2854-2862, 1999.
Stram Y., Meir R., Molad T., Blumenkranz R., Malkinson M., and Weisman Y., Avian Dis 38, 879-884, 1994.
Vakharia V.N., Ahamed B., and He J., Avian Dis 36, 736-742, 1992.
Sambrook J., Fritsch E.F., Maniatis T., Molecular Cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2000.
Yamaguchi T., Ogawa M., Inoshima Y., Miyoshi M., Fukushi H., and Hirai K., Virology 223, 219-223, 1996.
Brown M.D. and Skinner M.A., Virus Res 40, 1-15, 1996.
Chong L.K., Omar A.R., Yusoff K., Hair-Bejo M., and Aini I., Acta Virol 45, 217-226, 2001.
Boot H.J., ter Huurne A.A.H.M., Hoekman A.J.W., Peeters B.P.H., and Gielkens A.L.J., J Virol 74, 6701-6711, 2000.
Kibenge F.S.B., Jackwood D.J., and Mercado C.C., J Gen Virol 71, 569-577, 1990.
Bayliss C.D., Spies U., Shaw K., Peters R.W., Papageorgiou A., Muller H., and Boursnell M.E.G., J Gen Virol 71, 1303-1312, 1990.
Vakharia V.N., He J., Ahamed B., and Snyder D.B., Virus Res 31, 265-273, 1994.
Akin A., Wu C.C., and Lin T.L., J Virol Meth 82, 55-61, 1999.
Boot H.J., ter Huurne A.A.H.M., Peeters B.P.H., and Gielkens A.L.J., Virology 265, 330-341, 1999.
Hudson P.J., McKern N.M., Power B.E., and Azad A.A., Nucleic Acids Res 14, 5001-5012, 1986.
Kwon H.M., Kim D.K., Hahn T.W., Han J.H., and Jackwood D.J., Avian Dis 44, 691-696, 2000.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kim, TK., Yeo, SG. Cloning and Nucleotide Analysis of Segment A Gene of Infectious Bursal Disease Virus Detected in Korea. Virus Genes 26, 97–106 (2003). https://doi.org/10.1023/A:1022342524830
Issue Date:
DOI: https://doi.org/10.1023/A:1022342524830