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Inhibitory effect of menaquinone-7 (vitamin K2) on the bone-resorbing factors-induced bone resorption in elderly female rat femoral tissues in vitro

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Abstract

The inhibitory effect of menaquinone-7 (MK-7; vitamin K2) on osteoclast-like cell formation and osteoclastic bone resorption in vitro is found (Mol Cell Biochem 228: 39–47, 2001). This study, furthermore, was undertaken to determine the effect of MK-7 on the bone-resorbing factor-induced bone resorption using the femoral-diaphyseal and -metaphyseal tissues obtained from elderly female rats in vitro. Femoral-diaphyseal and -metaphyseal tissues were cultured for 48 h in Dulbecco's modified Eagle's medium (high glucose, 4.5%) supplemented with antibiotics and bovine serum albumin. The experimental cultures contained MK-7 (10−7–10−5 M). The bone-resorbing factors, parathyroid hormone (1–34) (PTH; 10−7 M) and prostaglandin E2 (PGE2; 10−5 M), caused a significant decrease in calcium content in the diaphyseal and metaphyseal tissues. The PTH or PGE2-induced decrease in bone calcium content was completely inhibited in the presence of MK-7 (10−7–10−5 M). In addition, MK-7 (10−7–10−5 M) completely prevented the PTH (10−7 M)- or PGE2 (10−5 M)-induced increase in medium glucose consumption and lactic acid production by bone tissues. These results support the view that MK-7 has a direct inhibitory effect on the bone-resorbing factor-induced bone resorption in bone culture using female aged femoral tissues in vitro.

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Authors and Affiliations

  1. Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka, 422-8526, Japan

    Masayoshi Yamaguchi & Satoshi Uchiyama

  2. Central Research Institute, Mitsukan Group Corporation, 2-6 Nakamura-cho, Handa, 475-8585, Japan

    Yoshinori Tsukamoto

Authors
  1. Masayoshi Yamaguchi
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  2. Satoshi Uchiyama
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  3. Yoshinori Tsukamoto
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Yamaguchi, M., Uchiyama, S. & Tsukamoto, Y. Inhibitory effect of menaquinone-7 (vitamin K2) on the bone-resorbing factors-induced bone resorption in elderly female rat femoral tissues in vitro . Mol Cell Biochem 245, 115–120 (2003). https://doi.org/10.1023/A:1022818111655

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