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Ontogenic profile of some antioxidants and lipid peroxidation in human placental and fetal tissues

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Abstract

Reactive oxygen species (ROS) pose a serious threat to maternal and fetal health during pregnancy. However, there is little information on the oxidative damage caused by ROS and its protection during prenatal life. The present study highlights the status of various antioxidants in human placental and fetal tissues at different phases of gestation. The activity profile of scavenging enzymes, superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase as well as the concentrations of non-enzymatic antioxidants, ascorbic acid, α-tocopherol, bilirubin and glutathione have been determined in human placental whole homogenate, placental brush border membrane and fetal liver over gestational periods ranging from 6 weeks of pregnancy till birth. The ontogenic profile of lipid peroxidation, a marker of oxidative damage has also been investigated in the feto-placental system. Catalase, superoxide dismutase and glutathione reductase activities increased significantly, but glutathione peroxidase activity remained almost the same throughout development. Except α- tocopherol and bilirubin, the concentrations of other non-enzymic scavengers followed a significant increasing trend with advancement of pregnancy. Results indicate that there is gradual suppression of lipoperoxide formation with the progress of gestation to protect the fetus against oxygen toxicity.

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References

  1. Halliwell B, Gutteridge JM: Role of free radicals and catalytic metal ions in human disease, an overview. Meth Enzymol 186: 1–85, 1990

    Google Scholar 

  2. Sies H, deGroot H: Role of reactive oxygen species in cell toxicity. Toxicol Lett 64: 547–551, 1992

    Google Scholar 

  3. Williams JW, Cunningham FG, MacDonald PC, Gant NF: The placenta and fetal membranes. In: J.W. Williams (ed). Williams Obstetrics. Appleton and Lange, Norwalk, Connecticut, USA, 1989, pp 39

    Google Scholar 

  4. Fantel AG, Mackler B, Stamps DL, Tran TT, Person RE: Reactive oxygen species and DNA oxidation in fetal rat tissues. Free Radic Biol Med 25: 95–103, 1998

    Google Scholar 

  5. Poranen AK, Ekblad U, Uotila P, Ahotupa M: Lipid peroxidation and antioxidants in normal and pre-eclamptic pregnancies. Placenta 17: 401–405, 1996

    Google Scholar 

  6. Sivan E, Lee Y, Wa YK, Reece EA: Free radical scavenging enzymes in fetal dysmorphogenesis among offspring of diabetic rats. Teratology 56: 343–349, 1997

    Google Scholar 

  7. Bohles H: Antioxidative vitamins in prematurely and maturely born infants. Int J Vit Nutr Res 67: 321–328, 1997

    Google Scholar 

  8. Gopinathan V, Miller NJ, Milner AD, Rice-Evans CA: Bilirubin and ascorbate antioxidant activity in neonatal plasma. FEBS Lett 349: 197–200, 1994

    Google Scholar 

  9. Langley SC, Kelly FJ: Depletion of pulmonary glutathione using diethylmaleic acid accelerates the development of oxygen-induced lung injury in term and preterm guinea pig neonates. J Pharm Pharmacol 46: 98–102, 1994

    Google Scholar 

  10. Qanungo S, Sen A, Mukherjea M: Antioxidant profile in human placental brush border membrane and umbilical cord blood. Clin Chim Acta 285: 1–12, 1999

    Google Scholar 

  11. Watson AL, Palmer ME, Jauniaux E, Burton GJ: Variations in expression of copper/zinc superoxide dismutase in villous trophoblast of the human placenta with gestational age. Placenta 18: 295–299, 1997

    Google Scholar 

  12. Stefanini S, Serafini B, Cimini A, Sartori C: Differentiation of kidney cortex peroxisomes in fetal and newborn rats. Biol Cell 82: 185–193, 1994

    Google Scholar 

  13. Guajardo L, Beharry KD, Modanlou HD, Aranda JV: Ascorbic acid concentration in umbilical cord veins and arteries of preterm and term newborns. Biol Neonate 68: 1–9, 1995

    Google Scholar 

  14. Mohan P, Ling SC, Watkins JB: Ontogeny of hepatobiliary secretion: role of glutathione. Hepatology 19: 1504–1512, 1994

    Google Scholar 

  15. Sen A, Mukherjea M: Modulation of bilayer fluidity by lipid peroxidation of human placental syncytiotrophoblast membranes during embryogenesis. Ind J Biochem Biophys 35: 216–233, 1998

    Google Scholar 

  16. Chaudhuri D, Kushari J, Mukherjea M: Occurrence of phosphodiesterase IV in the developing human brain, liver and placenta. Eur J Obstet Gynecol Reprod Biol 13: 309–316, 1982.

    Google Scholar 

  17. Balkovetz DF, Leibach FH, Mahesh VB, Devoe LD, Ganapathy V: Na+-H+ exchanger of human placental brush border membrane: Identification and characterisation. Am J Physiol 251: 852–860, 1982

    Google Scholar 

  18. Shirazi SP, Beechay RB, Butterworth PJ: The use of potent inhibitors of alkaline phosphatase to investigate the role of the enzyme in intestinal transport of inorganic phosphate. Biochem J 194: 803–809, 1981

    Google Scholar 

  19. Colas B, Maroux S: Simultaneous isolation of brush border and basolateral membrane from rabbit enterocytes. Presence of brush border hydrolases in basolateral membrane of rabbit enterocytes. Biochim Biophys Acta 600: 406–420, 1980

    Google Scholar 

  20. Veeger C, Der Vartanian DV, Zeylemaker WP: Succinate dehydrogenase. Meth Enzymol 13: 81–90, 1969

    Google Scholar 

  21. Dallner G, Siekevitz P, Palade GE: Biogenesis of reticulum membranes-II. Synthesis of constitutive microsomal enzymes in developing rat hepatocytes. J Cell Biol 30: 97–117, 1966

    Google Scholar 

  22. Paoletti F, Mocali A: Determination of superoxide dismutase activity by purely chemical system based on NAD(P) H oxidation. Meth Enzymol 186: 209–220, 1990

    Google Scholar 

  23. Beers RF Jr, Sizer IWA: A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195: 133, 1952

    Google Scholar 

  24. Rotruck JT, Pope AC, Ganther HE, Swanson AB, Hoekstra NG: Selenium: Biochemical role as a component of glutathione peroxidase. Science 179: 588–590, 1973

    Google Scholar 

  25. Delides A, Spooner RJ, Goldbery DM, Neal FE: An optimised semiautomatic rate method for serum glutathione reductase activity and its application to patients with malignant disease. J Clin Pathol 29: 73–77, 1976

    Google Scholar 

  26. Desai ID: Vitamin E analysis methods for animal tissues. Meth Enzymol 105: 138–147, 1984

    Google Scholar 

  27. Omaye ST, Turnabull JD, Sauberlich HE: Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids. Meth Enzymol 62: 1–11, 1979

    Google Scholar 

  28. Malloy HA, Evelyn KA: The determination of bilirubin with the photoelectric colorimeter. J Biol Chem 119: 481, 1937

    Google Scholar 

  29. Beutler E, Duron O, Kelly BM: Improved method for the determination of blood glutathione. J Lab Clin Med 61: 882–888, 1963

    Google Scholar 

  30. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ: Protein measurement with folinphenol reagent. J Biol Chem 193: 265–275, 1951

    Google Scholar 

  31. Cyanomon AA, Isenberg JN, Nguyen WH: Erythrocyte malondialdehyde release in vitro: A functional measure of vitamin A status. Clin Chim Acta 151: 169–176, 1985

    Google Scholar 

  32. Znamenskaia TK: Role of antioxidant enzymes of the feto-placental complex in neonatal adaptation to maternal diabetes mellitus. Ukr Biokhim Zh 66: 93–97, 1994

    Google Scholar 

  33. Cester N, Staffolani R, Rabini RA, Magnanelli R, Romanini C: Pregnancy induced hypertension: A role for peroxidation in microvillous plasma membranes. Mol Cell Biochem 131: 151–155, 1994

    Google Scholar 

  34. Mayes PA: Lipid transport and storage. In: R.X. Murray, D.X. Granner, P.A. Mayes, V.W Rodwell (eds). Harper's Biochemistry. Appleton and Lange, Norwalk, Connecticut, USA, 1990, pp 234–298

    Google Scholar 

  35. Meister A, Anderson ME: Glutathione. Ann Rev Biochem 52: 711–760, 1983

    Google Scholar 

  36. Yoshioka T, Ando M, Taniguchi K, Yamasaki F, Motoyama H: Lipoperoxidation and antioxidant substances in the human placenta during gestation. Nippon Sanka Fujinka Gakkai Zasshi 42: 1634–1640, 1990

    Google Scholar 

  37. Rall TW, Lehninger AL: Glutathione reductase of animal tissues. J Biol Chem 194: 119–130, 1952

    Google Scholar 

  38. Weeger I, Pallrdan B: Vitamin C deficiency causes hematological and skeletal abnormalities during fetal development in swine. J Nutr 124: 241–248, 1994

    Google Scholar 

  39. May JM, Qu ZC, Mendiratta S: Protection and recycling of alpha tocopherol in human erythrocytes by intracellular ascorbic acid. Arch Biochem Biophys 349: 281–289, 1998

    Google Scholar 

  40. Srivastava S, Phadke RS, Govil G, Rao CNR: Fluidity, permeability and antioxidant behaviour of model membranes incorporated with α-tocopherol and Vitamin E acetate. Biochim Biophys Acta 734: 353–362, 1983

    Google Scholar 

  41. Siman CM, Eriksson UJ: Vitamin E decreases the occurrence of malformations in the offspring of diabetic rats. Diabetes 46: 1054–1061, 1997

    Google Scholar 

  42. Stocker R, Glazer AN, Ames BN: Antioxidant activity of albumin-bound bilirubin. Proc Natl Acad Sci 84: 5918–5922, 1987

    Google Scholar 

  43. Navarro J, Obrador E, Carretero J, Petschen I, Avino J, Estrela JM: Changes in glutathione status and the antioxidant system in blood and in cancer cells associate with tumour growth in vivo. Free Radic Biol Med 26: 410–418, 1999

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, n, University College of Science, 35 Ballygunge Circular Road, Calcutta, 700 019, India

    Suparna Qanungo

  2. Department of Biochemistry, University College of Science, 35 Ballygunge Circular Road, Calcutta, 700 019, India

    Manju Mukherjea

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  1. Suparna Qanungo
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Qanungo, S., Mukherjea, M. Ontogenic profile of some antioxidants and lipid peroxidation in human placental and fetal tissues. Mol Cell Biochem 215, 11–19 (2000). https://doi.org/10.1023/A:1026511420505

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