Abstract
Generally, heat inactivated foetal calf serum (FCS) containing media are used for the cultivation of animal and human cells. The role of serum source and serum treatment on the behaviour of cells has long been neglected. The present study was performed to investigate the effects of serum heat inactivation and serum source on trabecular bone derived progenitor cells (HBC). Furthermore, it was investigated in how far these reactions differed from those seen in bone marrow derived mesenchymal progenitor cells (HBMC) cultures. We found that HBC cultures performed differently in the presence of FCS and HS with or without heat inactivation. The reactions similar to some degree those observed in HBMC cultures. The implications of the results on cell–implant surface interaction studies are discussed.
Similar content being viewed by others
References
R. L. Ward, J. Clin. Microbiol. 6 (1979) 650.
A. Bruinink, M. Hälg, U. Tobler and J. Grünert, Eur. J. Cell Biol. (2004) submitted.
V. Sottile, C. Halleux, F. Bassilana, H. Lkeller and K. Seuwen, Bone 30 (2002) 699.
M. E. Nuttall, A. J. Patton, D. L. Olivera, D. P. Nadeau and M. Gowen, J. Bone Miner. Res. 13 (1998) 371.
F. Debais, M. Hott, A. M. Graulet and P. J. Marie, ibid. 13 (1998) 645.
M. Kassem, L. Mosekilde and E. F. Eriksen, Eur. J. Endocrinol. 130 (1994) 381.
I. Shur, F. Lokiec, I. Bleiberg and D. Benayahu, J. Cell Biochem. 83 (2001) 547.
A. Bruinink, in “The Brain in Bits and Pieces”, edited by G. Zbinden (MTC Verlag, 1992) p. 23.
A. Muraglia, I. Martin, R. Cancedda and R. Quarto, Bone 22 (1998) 131S.
J. A. Galligher, R. Gundle and J. N. Beresford in “Methods in Molecular Medicine: Human Cell Culture Protocols”, edited by G. E. Jones (Hamana Press Inc., 1996) p. 233.
A. Bruinink and E. Wintermantel, Biomaterials 22 (2001) 2465.
N. Yamamoto, M. Isobe, A. Negishi, H. Yoshimasu, H. Shimokawa, K. Ohya, T. Amagasa and S. Kasugai, J. Med. Dent. Sci. 50 (2003) 63.
R. O. C. Oreffo, A. S. Virdi and J. T. Triffitt, Eur. J. Cell Biol. 74 (1997) 251.
M. G. Mcalinden and D. J. Wilson, Cell Transplant. 9 (2000) 445.
S. A. Kuznetsov, M. H. Mankani and P. G. Robey, Transplantation 70 (2000) 1780.
F. P. Huang and D. I. Stott, Lupus 4 (1995) 297.
M. Baddoo, K. Hill, R. Wilkinson, D. Gaupp, C. Hughes, G. C. Kopen and D. G. Phinney, J. Cell. Biochem. 89 (2003) 1235.
M. J. Coelho and M. H. Fernandes, Biomaterials 21 (2000) 1095.
P. Clark, P. Connolly, A. S. G. Curtis, J. A. T. Dow and C. D. W. Wilkinson, Development 108 (1990) 635.
E. Bess, R. Cavin, K. Ma and J. L. Ong, Implant Dent. 8 (1999) 126.
P. B. Van Wachem, C. M. Vreriks, T. Beugeling, J. Feijen, A. Bantjes, J. P. Detmers and W. G. Van Aken, J. Biomed. Mater. Res. 21 (1987) 701.
J. G. Steele, B. A. Dalton, G. Johnson and P. A. Underwood, Biomaterials 16 (1995) 1057.
D. J. Giard, In Vitro Cell. Devel. Biol. 23 (1987) 691.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Bruinink, A., Tobler, U., Hälg, M. et al. Effects of serum and serum heat-inactivation on human bone derived osteoblast progenitor cells. Journal of Materials Science: Materials in Medicine 15, 497–501 (2004). https://doi.org/10.1023/B:JMSM.0000021127.62879.a1
Issue Date:
DOI: https://doi.org/10.1023/B:JMSM.0000021127.62879.a1