Abstract
p53 triggers apoptosis in response to cellular stress. We analyzed p53-dependent gene and protein expression in response to hypoxia using wild-type p53-carrying or p53 null HCT116 colon carcinoma cells. Hypoxia induced p53 protein levels and p53-dependent apoptosis in these cells. cDNA microarray analysis revealed that only a limited number of genes were regulated by p53 upon hypoxia. Most classical p53 target genes were not upregulated. However, we found that Fas/CD95 was significantly induced in response to hypoxia in a p53-dependent manner, along with several novel p53 target genes including ANXA1, DDIT3/GADD153 (CHOP), SEL1L and SMURF1. Disruption of Fas/CD95 signalling using anti-Fas-blocking antibody or a caspase 8 inhibitor abrogated p53-induced apoptosis in response to hypoxia. We conclude that hypoxia triggers a p53-dependent gene expression pattern distinct from that induced by other stress agents and that Fas/CD95 is a critical regulator of p53-dependent apoptosis upon hypoxia.
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Main
The tumor suppressor p53 regulates cellular processes such as cell cycle progression, DNA repair and apoptosis.1 p53 is stabilized and activated in response to different types of cellular stress, for example, DNA damage, oncogenic signalling and hypoxia, and induces a biological response through transcriptional regulation of downstream target genes. The molecular mechanisms that dictate the decision of a cell to enter growth arrest or undergo apoptosis or both in response to p53 activation are only partially understood. Also, it remains unclear why different cell types display differential response to functional p53. The biological outcome of p53 activation presumably depends on several factors, including cellular context, cell type and type of stress agent.1 It is plausible that p53 activates different specific and possibly overlapping set of target genes in response to different stress signals. Therefore, knowledge about the target genes and their regulation under different conditions is vital for the understanding of p53-mediated tumor suppression.
Previous DNA microarray studies have allowed a global analysis of p53-dependent gene expression in response to p53 activation by various means.2, 3 These studies have indicated that several hundred genes are potentially regulated by p53. Moreover, in silico analysis of the human genome sequence has revealed that up to 4800 genes contain one or more potential p53 binding sites.4, 5 Thus, it is conceivable that a large number of genes are regulated by p53, although not necessarily in the same cell in response to the same stress signal. Microarray studies have so far relied on artificial cell systems for activation of p53, for example, the metallothionein promoter or expression of exogenous p53. In addition, the numbers of genes on the arrays used have been relatively low. Thus, further studies of p53-dependent gene expression based on activation of endogenous p53 in response to physiological cellular stress should provide more reliable information about p53-dependent stress responses.
Hypoxia is a stress condition that probably has a great impact on tumor development in vivo. The hypoxic microenvironment in a solid tumor before the establishment of a sufficient blood supply may induce a p53 response and thus impose a selection for p53 point mutation. Hypoxia or anoxia has been shown to induce p53 accumulation and apoptosis.6, 7 However, p53 activation by hypoxia does not appear to induce expression of classical p53 target genes such as p21, MDM2 and Bax, but can still mediate transrepression of certain genes such as α-tubulin.8 This indicates that activation of p53 in response to hypoxia leads to a gene expression pattern that is different from that induced by p53 in response to γ-irradiation, ultraviolet (UV) light and other DNA-damaging agents.
Here, we present the first analysis of p53-dependent gene expression patterns in hypoxia-treated human cells using cDNA microarrays. We have found that hypoxia does not induce most classical p53 target genes but induces several genes that were previously not known to be regulated in a p53-dependent manner. Moreover, we found that the death receptor Fas/CD95 is upregulated by p53 in response to hypoxia and plays a major role in hypoxia-induced p53-dependent apoptosis.
Results
p53-dependent apoptosis in hypoxia-treated HCT116 cells
To determine whether hypoxia induces p53 protein accumulation in HCT116 wtp53+/+ cells, we cultured the cells at 0.3% O2 and examined p53 protein levels by Western blotting at 8 and 16 h. p53 was significantly induced under hypoxic conditions and accumulated in the nucleus (Figure 1a, b). We obtained the same results with 0.05% O2 (data not shown). A similar induction of p53 was observed after treatment with UV (12.5, 25 or 50 mJ/cm2), or γ-irradiation (5, 10 or 20 Gy) (data not shown). Western blot analysis demonstrated that hypoxia causes p53 Ser-15 phosphorylation (Figure 1c), in agreement with a previous report.9 To examine hypoxia-induced apoptosis in the presence or absence of wild-type (wt) p53, HCT116 cells were grown at 0.3% O2 for 24 h and analyzed by TUNEL staining. We observed a significant increase in the fraction of TUNEL-positive cells in the HCT116 wtp53+/+ cultures upon hypoxia treatment, but not among the p53 null HCT116 cells (Figure 1d). Only a few TUNEL-positive cells were observed in both cell lines under normoxic growth conditions. Similar results were obtained with 0.05% O2 (data not shown). We confirmed hypoxia-induced p53-dependent apoptosis at 36 and 48 h by propidium iodide staining and flow cytometry (see below; Figure 5c, d). Thus, hypoxia induces p53-dependent apoptosis in the HCT116 cells.
Microarray analysis of p53-dependent gene expression in response to hypoxia
We performed microarray analysis to study gene expression in HCT116 wtp53+/+ and HCT116 p53 null cells upon hypoxia treatment (0.05% O2) for 8 and 16 h We observed altered transcription levels of several thousand genes in response to hypoxia in both HCT116 cell lines, whereas p53-dependent changes in expression were limited to a few hundred genes. With our criteria for significant differential expression (see Materials and Methods), the estimated total number of hypoxia-dependent differentially expressed genes was 365, of which 27 were p53-dependent (Table 1). This indicates that 8% of the transcripts undergoing hypoxia-induced changes in abundance are affected by wtp53. Among the 27 genes found to be regulated by hypoxia in a p53-dependent manner, 22 are known genes and five are expressed sequence tags (ESTs) of unknown origin (indicated as ‘data not found’ in Table 1). Sixteen genes were regulated at the 8 h time point, whereas another 11 genes only showed differential expression at 16 h of hypoxia. Prominin1 and Muscleblind-like 2 were upregulated at both 8 and 16 h. The majority of the p53-dependent differentially expressed genes (24 out of 27) were upregulated in the hypoxia-treated cells. HIAP1 (apoptosis inhibitor 2)/BIRC3, and two unknown ESTs were repressed in a p53-dependent manner, and SLC2A3 showed significantly stronger induction in the p53 null cells at 8 h of hypoxia. Most of the identified p53-regulated genes carry one or several potential p53 DNA binding motifs. There was no apparent correlation between the score of the identified motifs according to the p53MH algorithm4 and the degree of p53-dependent gene expression. For instance, angiomotin-like 2 (AMOTL2) which has a score of <0.85 was markedly induced at 8 h of hypoxia (Table 1). Moreover, the p53-repressed gene HIAP1/BIRC3 also contains potential p53 binding sites.
Only one of the identified p53-regulated genes, the death receptor TNFRS6 or Fas/CD95, is a previously known p53 target gene. According to the microarray analysis, Fas/CD95 was induced 2.4-fold after 16 h of hypoxia treatment in the HCT116 wtp53+/+ cells. Fas/CD95 was not induced in the p53 null HCT116 cells at this time point, and was in fact slightly repressed at 8 h.
The list of genes that shows p53-dependent expression upon hypoxia includes several genes that may be involved in regulation of cell growth, DNA-damage responses or apoptosis, for example, ANXA1 (apoptosis), DDIT3, also known as GADD153 or CHOP (DNA damage), SMURF1 and SMURF2 (regulators of the TGF-β pathway) and HIAP1/BIRC3 (apoptosis) (see Discussion).
We specifically addressed the expression of the 283 genes on the array that are involved in apoptosis according to the Gene Ontology nomenclature (Figure 2). Twelve of these showed significant differential expression in response to hypoxia but only two, Fas/CD95 and HIAP1/BIRC3, were regulated in a p53-dependent manner. Fas/CD95 was significantly induced (see above) and HIAP1/BIRC3 was significantly repressed at 8 h.
General hypoxia-induced changes in transcription levels
The vast majority (87%) of the 365 genes that were affected by hypoxia treatment were upregulated. The main molecular mediator of the hypoxic response is the transcription factor hypoxia inducible factor-1 (HIF1). HIF1 target genes are mainly involved in processes related to adaptation to the hypoxic environment such as angiogenesis, glycolysis and erythropoiesis. We did not detect any upregulation of HIF1 at the transcriptional level, in agreement with earlier studies.10 A large number of genes with significantly altered mRNA levels in our study are already known to be hypoxia-regulated with characterized HIF1 promoter binding sites, for example, the main mediator of angiogenesis, VEGF, the proapoptotic gene BNIP3L and the endoplasmic reticulum oxidoreductin ERO1L, confirming the validity of our results. However, genes previously not associated with hypoxia were also identified, for example, the transcription factor JMJD1A, which is a negative regulator of cell growth signalling, and the ubiquitin-conjugating enzyme UBE2J1 (Supplementary Table 1 and Figure 1a–d).
Confirmation of microarray data by Northern blotting, reverse transcription-polymerase chain reaction and Western blotting
To confirm and extend the cDNA microarray data, we examined mRNA and/or protein expression of genes that showed p53-dependent expression in hypoxia-treated HCT116 cells according to the microarray analysis, as well as several known p53 target genes that were not represented on the array (Table 2). Northern blotting and quantitative reverse transcription-polymerase chain reaction (RT-PCR) confirmed significant p53-dependent induction of Fas/CD95 mRNA after 8 and 16 h of hypoxia treatment, demonstrating that Fas/CD95 induction is due to transcriptional transactivation (Figure 3a). We also confirmed p53-dependent induction of Fas/CD95 by Western blotting (Figure 3b), immunostaining (Figure 3d) and flow cytometry (Figure 3e, f). Likewise, we confirmed p53-dependent induction of AMOTL2 mRNA at 8 h and DDIT3/CHOP and SMURF1 protein at 16 h after hypoxia treatment (Figure 3a, b). Quantitative RT-PCR confirmed that SEL1L and SGTB were induced by hypoxia in a p53-dependent manner at 8 and/or 16 h (Table 2).
To determine if Fas/CD95 is induced by hypoxia in normal cells, we treated human diploid fibroblasts (HDFs) with hypoxia and examined Fas/CD95 expression by Western blotting. Figure 3c shows that Fas/CD95 protein levels were markedly increased in these cells after 24 and 48 h of hypoxia. Hypoxia also induced Fas/CD95 protein expression in the wtp53-carrying LnCAP prostate carcinoma cells (Figure 3g). MCF-7 cells expressed low or undetectable levels of Fas/CD95 both before and after hypoxia treatment, and U2OS cells expressed high levels of Fas/CD95 even at normoxia. Hypoxia did not induce Fas/CD95 in SW480 cells carrying mutant p53 (Figure 3g). Neither p21 nor PUMA were induced by hypoxia in any of the tested cells (Figure 3g).
To confirm that Fas/CD95 is a direct transcriptional target of p53 in response to hypoxia, we measured Fas/CD95 promoter activity in hypoxia-treated HCT116 cells using a luciferase reporter assay. This revealed a pronounced increase in luciferase activity upon hypoxia treatment (Figure 3h). We did not detect any increase in luciferase activity in hypoxia-treated HCT116 cells transfected with the same luciferase reporter construct containing the p21 promoter (Figure 3h). We conclude that Fas/CD95 is upregulated at the transcriptional level in response to hypoxia.
Our further analysis demonstrated that none of several known p53 target genes, including p21, GADD45, Noxa, Cathepsin D and PUMA, were induced by hypoxia in a p53-dependent manner at 8 or 16 h (Figure 4a, b; Table 2). Northern blotting and densitometric quantification revealed either no significant changes in expression levels in response to hypoxia or a similar induction in both p53 null- and wtp53-expressing HCT116 cells. MDM2 mRNA was induced in both cell lines, but we observed a somewhat stronger induction in the wtp53-expressing cells at 16 h (Figure 4a; Table 2). This was even more pronounced in the Western blot analysis (Figure 4b; Table 2).
As a control, we treated the HCT116 cells with γ-irradiation and examined expression of the p53-target proteins p21, MDM2, PUMA and Fas/CD95 by Western blotting. All four proteins showed p53-dependent induction in response to γ-irradiation (Figure 4c).
In addition to transactivation of target genes, p53 can repress transcription of certain genes, including Bcl-2 and hTERT. We therefore tested if hypoxia-induced p53 was able to affect expression of these two genes. As shown in Figure 4b, hypoxia caused a p53-dependent downregulation of both Bcl-2 and hTERT protein levels.
Fas/CD95 is required for p53-dependent apoptosis induced by hypoxia
Our finding that Fas/CD95 belongs to a limited set of genes that are induced by hypoxia in a p53-dependent manner raised the possibility that Fas/CD95 and the death receptor pathway plays an important role in the p53-dependent apoptotic response to hypoxia. To test this, we treated HCT116 wtp53+/+ cells with an anti-Fas/CD95 antibody (APO-1) that blocks Fas/CD95 signalling and subsequently exposed the cells to hypoxia. We counted TUNEL-positive cells in both untreated and hypoxia-treated cultures in the presence or absence of the blocking antibody. We found that the cells treated with blocking anti-Fas/CD95 antibody were highly resistant to hypoxia-induced apoptosis (Figure 5a, b). We also treated HCT116 wtp53+/+ cells with a caspase 8 inhibitor (Z-IETD) to block the death receptor pathway before hypoxia treatment. Again, hypoxia-induced apoptosis was greatly reduced (Figure 5a, b). To confirm these results, we assessed apoptosis at 36 h of hypoxia by propidium iodide staining and flow cytometry. We observed a significant apoptosis in the HCT116 wtp53 +/+ cells (Figure 5c, d), which was blocked by either anti-Fas/CD95 antibody or the caspase 8 inhibitor (Figure 5c, d). Similar results were obtained at 48 h (data not shown). Thus, we conclude that Fas/CD95 signalling is critical for the p53-dependent apoptotic response to hypoxia in these cells.
Discussion
Hypoxia has been shown to trigger p53 accumulation and p53-dependent apoptosis in several systems.6, 11, 12 We asked whether hypoxia would trigger a p53-dependent gene expression pattern distinct from those induced by other stress agents, such as γ-irradiation and UV. Our analysis revealed significant p53-dependent induction or repression of only 27 genes among the 12 454 different genes represented on the microarray. One known p53 target gene, Fas/CD95, showed significant p53-dependent induction. Further analysis by Northern blotting, RT-PCR and/or Western blotting confirmed that many p53 targets were not regulated in a p53-dependent manner in hypoxic cells. Some p53 target genes, such as p21 and KILLER/DR5, showed p53-independent upregulation in hypoxic cells. MDM2 also showed p53-independent regulation at both the mRNA and protein levels, but its induction in the wtp53-expressing cells was more pronounced than in the p53 null cells. Thus, although exposure to hypoxic conditions did trigger p53 accumulation and p53-dependent apoptosis, it induced a gene expression pattern that is considerably different from those induced by activation of exogenous wtp53,2, 3 or DNA-damaging agents such as γ-irradiation.
Our finding that hypoxia does not induce p53-dependent expression of many p53 target genes is in agreement with a previous study showing that p53 activation by hypoxia mainly triggers p53-mediated transrepression via association of p53 with mSin3A and p53-mediated transrepression.8 However, it is evident from our results that hypoxia not only induces p53-dependent transrepression but also p53-dependent transactivation of a limited set of genes, many of which carry p53 binding motifs. Thus, one important conclusion from our study is that hypoxia-induced p53 is clearly capable of both transcriptional transactivation and transrepression. Notably, we observed p53-dependent induction of both MDM2 mRNA and protein in response to hypoxia, whereas others have reported that hypoxia induces MDM2 downregulation.8, 13
We demonstrate that Fas/CD95 is a critical p53 target in the hypoxia response, since Fas/CD95 is markedly upregulated by p53 in hypoxic cells and blocking Fas/CD95 signalling completely abrogated hypoxia-induced apoptosis. In addition, Fas/CD95 protein was also induced by hypoxia both in normal HDFs and in human prostate carcinoma cells. This highlights Fas/CD95 as a key effector of p53-induced apoptosis in response to hypoxia. Further work is needed to determine whether Fas/CD95 plays a similarly crucial role in the response to hypoxia in other cell types than those tested here. It remains unclear why Fas/CD95, but not p21, GADD45, PIG3 and other well-known p53 target genes, is induced in a p53-dependent manner in response to hypoxia. However, it is interesting to note that Fas/CD95 has a HIF1-responsive element (HRE) located 1550 bp 5′ of the transcription start site. The sequence of this HRE, TACGTGGG, matches the consensus sequence BRCGTGB, where B=C, T or G, and R=A or G.14 MDM2 also has an HRE (at position −2929), but this HRE (GACTTGGG) does not show a complete match with the consensus HRE. Thus, it is conceivable that HIF1 could act as a cofactor for p53-mediated transactivation in hypoxic cells.
Our analysis revealed a number of previously unknown p53 target genes that showed increased or decreased p53-dependent expression upon exposure to hypoxia. Several of these genes may have roles in processes such as growth control, maintenance of genome integrity, apoptosis and cancer. ANXA1 (ANNEXIN 1) is a member of the annexin family of calcium- and phospholipid-binding proteins and has proapoptotic effect in circulating neutrophils15 and rat thymocytes.16 The observation that ANXA1 is expressed on the cell surface suggests that it may constitute an ‘eat me’ signal for removal of apoptotic cells.17 ANXA1 induces BAD dephosphorylation, allowing BAD to associate with Bcl-XL, which triggers the mitochondrial apoptosis pathway.15 ANXA1 has a p53 DNA binding motif located 1049 bp from the transcription start site that matches the p53 consensus binding sites at 18 out of 20 positions (Table 1). Thus, p53-dependent upregulation of ANXA1 in hypoxic cells could contribute to the apoptotic response.
The DDIT3 gene, also known as GADD153 or CHOP, encodes an inhibitor of the transcription factor C/EBP and is induced by DNA damage.18 DDIT3 is frequently rearranged in myxoid liposarcomas owing to a characteristic t(12;16)(q13;p11) chromosomal translocation resulting in a fusion gene in which the RNA-binding domain of TLS is replaced by the DNA-binding domain and leucine zipper of DDIT3.19 Elevated DDIT3 expression was shown to sensitize cells to ER stress through downregulation of Bcl-2 and increased production of reactive oxygen species.20 A recent study showed that DDIT3 is induced in hypoxia-treated rat embryonic heart-derived cells. Overexpression of DDIT3 sensitized these cells to hypoxia-induced apoptosis, whereas antisense GADD153 had the opposite effect.21 Like ANXA1, DDIT3 has at least one p53 binding site (Table 1).
The SEL1L gene shares sequence homology with the Caenorhabditis elegans sel-1 gene, an extragenic suppressor of the lin-12 hypomorphic mutant.22 SEL1L has been shown to inhibit Notch, and its yeast homolog IBD2 is involved in the Bub2p-dependent spindle checkpoint.23 Human SEL1L is mainly expressed in the pancreas of healthy individuals but not in a significant fraction of pancreatic adenocarcinomas.24 Overexpression of SEL1L in pancreatic carcinoma cells caused decreased clonogenicity and anchorage-independent growth, and inhibited tumor xenograft growth in nude mice. The same study also indicated that SEL1L regulates expression of genes involved in cellular growth control, possibly through the TGF-β signalling pathway.25 We identified at least one potential p53 binding site at position −2195 in the SEL1L gene (Table 1).
LATS2, the human homolog of the Drosophila large tumor suppressor gene, encodes a putative nuclear Ser/Thr kinase expressed in several human tissues. Homozygous inactivation of LATS2 in mice resulted in embryonic lethality and LATS2 null mouse embryo fibroblasts showed increased growth potential, cytokinesis defects and aneuploidy.26 Interestingly, LATS2 was shown to induce apoptosis through downregulation of the antiapoptotic Bcl-2 and Bcl-XL proteins.27
Other genes identified in our microarray analysis may also play roles in p53-dependent hypoxia-induced apoptosis. AMOTL2 was identified as a novel member of the angiomotin family.28 Angiomotin itself may promote angiogenesis by increasing cell motility.29 The exact function of AMOTL2 and its involvement in regulation of cell motility, growth and survival, if any, remains to be established. ASXL1 encodes a polycomb group protein belonging to the CXXC gene family that has been implicated in tumor development through transcriptional regulation of target genes.30 The product of the RBBP6 gene binds the retinoblastoma protein, a central regulator of cell cycle progression and cell survival.31 SMURF1 and SMURF2 both encode E3 ubiqutin ligases regulating Smad proteins in the TGF-β signalling pathway that control cell proliferation, differentiation, migration and survival.32 p53-dependent activation of SMURF1 and/or SMURF2 expression in response to hypoxia could lead to inhibition of this pathway and thus affect cell growth and survival.
In addition to these novel upregulated p53 target genes, we identified HIAP1/BIRC3 as a p53 repressed gene in hypoxia-treated cells. The HIAP1/BIRC3 protein was shown to inhibit apoptosis by interfering with caspase activation at the apoptosome; its displacement resulted in caspase 3 activation and release from the complex.33 HIAP1/BIRC3 inhibits caspase 8-dependent TRAIL/Apo2L-induced apoptosis, linking this protein to the death receptor apoptosis pathway.34 Thus, downregulation of HIAP1/BIRC3 may contribute to p53-induced apoptosis in response to hypoxia.
In conclusion, we have shown that hypoxia triggers a p53-dependent gene expression pattern that is distinct from those induced by other stress agents and involves both transcritional transactivation and transrepression of downstream targets. This demonstrates that the ability of p53 to regulate the gene expression is to a large extent dependent on the type of stress agent that triggers the p53 response. Different stress agents may presumably induce differential p53-dependent gene expression patterns via activation of specific cofactors that are required for transcriptional regulation of p53 target genes. HIF1 is obviously a possible cofactor in the case of hypoxia. Furthermore, post-translational modifications of p53, for example, phosphorylation, acetylation and sumoylation, could affect the ability of p53 to transactivate or repress target genes by modulating the interaction of p53 with co-activators or co-repressors such as p300 and mSin3A.8, 35 Importantly, our analysis has led to the identification of a number of new p53 target genes that may have essential roles in the control of cell growth, cell survival and genomic integrity. Further studies of these genes should provide important information about the p53 response to cellular stress and how p53 antagonizes tumor growth.
Materials and Methods
Cell culture and hypoxia treatment
Cells were grown in Iscove's modified Dulbecco's medium with 10% fetal bovine serum (FBS) (Invitrogen, UK), 2 mM L-glutamine (Sigma-Aldrich, Germany) and 50 μg/ml gentamicin (Sigma-Aldrich, Germany) at 37°C in 5% CO2. HCT116 human colon carcinoma cells carrying wtp53 (HCT116 wtp53+/+) and a p53 null subline (HCT116 p53−/−) were provided by Bert Vogelstein, Johns Hopkins University. Normal HDFs were provided by Anders Zetterberg, Karolinska Institute, and cultured in Dulbecco's modified Eagle's medium with 10% FBS, 50 μg/ml gentamicin and 2 mM L-glutamine. Experiments were carried out with HDFs at passage 12. An oxygen concentration of 0.3% was obtained by flushing a sealed box containing the cells with premixed gas composed of 95% nitrogen (N2) and 5% CO2 (AGA AB, Sweden). To obtain an oxygen concentration of 0.05%, the cells were flushed with the premixed gas continuously throughout the experiment.
RNA extraction
Total cellular RNA from HCT116 cells was prepared using the TRIzol reagent (Invitrogen, UK) from frozen cell pellets according to the manufacturer's protocol. RNA concentration was determined in a spectrophotometer and quality of total RNA was verified by electrophoresis on a 1% agarose gel.
Microarray analysis
The microarrays consisted of 20 352 cDNA fragments from the sequence-verified human clone collection 97001.V, plates 1–212 (Research Genetics, AL, USA), corresponding to 12 454 unique Unigene IDs. The cDNAs were spotted onto Ultra GAPS slides (Corning, NY, USA) with a QArray (Genetix, UK) instrument (for more information, see A-MEXP-254 at Array Express, www.ebi.ac.uk/arrayexpress). Target cDNA was generated by oligo dT-mediated RT of 15 μg of total RNA, indirectly labelled using amino-allyl-modified nucleotides, and hybridized as described (www.ktharray.se). RNA was extracted from HCT116 wtp53+/+ and p53 null cells at time points 0, 8 and 16 h of hypoxia treatment (0.05%). The microarray hybridizations were performed in triplicates including one dye-swap, 0–8, 8–16, and 16–0 h. The arrays were scanned at 532 and 635 nm with 10-μm resolution using a G2565BA DNA microarray scanner (Agilent Technologies, CA, USA). Image analysis was performed in GenePixPro 5.0 software (Axon instruments, CA, USA), where spots were removed from further analysis if less than 70% of foreground pixels were below background intensity plus two S.D. in both channels, or if the signal to noise ratio was below three in both channels, or if the difference between ratio of medians and regression ratio exceeded 20% in one of the channels. After removal of bad quality spots, the remaining intensities were print tip lowess normalized in the R environment for statistical computing (R Development Core Team, http://www.R-project.org) with the com.braju.sma package (Bengtsson H, http://www.maths.lth.se/publications). An empirical bayes method (B-test) implemented in the Limma package36 was used for ranking differentially expressed genes. For significant hypoxia-induced differential expression, the genes were to have B>0, ∣M∣>1 and P<0.01 comparing before and after treatment at each time point. In addition to significant hypoxia response, the genes were required to fulfill the same criteria for the comparisons between cell lines for significant hypoxia-induced p53 dependence. All microarray data are available at Array Express (www.ebi.ac.uk/arrayexpress).
Northern blot analysis and quantitative RT-PCR
RNA samples (30 μg) were fractionated on 1% agarose gels in 2.2 M formaldehyde, transferred onto a nylon membrane, and hybridized with 32P-labelled probes. Signals were recorded on an FLA-3000 phosphoimager (Fujifilm, Sweden). Densitometry was performed using Image Gauge version 3.12 analysis software (Fujifilm, Sweden). Quantitative RT-PCR was carried out with the iCycler iQ system with Supermix (Bio-Rad, CA, USA) according to the kit's protocol, or with Taqman probes with TaqMan® Universal PCR Master Mix (Applied Biosystems, CA, USA), according to the manufacturer's recommendations. Primer sequences are shown in Supplementary Table 2.
Antibodies, Western blotting, immunostaining and TUNEL
Anti-p53 antibody DO-1 and anti-p21 antibody 6B6 were from BD Bioscience (San Diego, CA, USA), anti-Ser-15-phosphorylated p53 antibody 16G8 was from Cell Signaling Technology (Beverly, MA, USA), anti-MDM2 antibody N-20 and anti-Smurf1 antibody were from Santa Cruz (San Diego, CA, USA), anti-Puma antibody ab9643 was from Abcam (Cambridge, UK), anti-Fas/CD95 antibody B10 was from Santa Cruz (San Diego, CA, USA), anti-Fas/CD95-blocking antibody APO-1 was from Nordic BioSite (Stockholm Sweden), mouse anti-hTERT antibody NCL-hTERT was from Novocastra (Newcastle, UK) and anti-β-actin antibody was from Sigma (St Louis, MO, USA). HCT116 cells were seeded on glass coverslips (105 cells/well), grown for 24 h and treated by hypoxia (0.3 or 0.05% O2). Western blotting and immunofluorescence staining were carried out as described by Lindstrom and Wiman.37 Staining was viewed using an Axioplan II (3D) microscope equipped with an AxioCam CCD camera (Carl Zeiss, Oberkochen, Germany). TUNEL was performed as described by Rahman et al.38 TUNEL-positive cells were counted in a defined field in four different areas of each slide to obtain a mean number. The experiments were performed with triplicate samples.
Luciferase reporter assay
HCT116 cells were seeded in 24-well plates (6 × 104 cells/well) 24 h before transfection and transfected with 100 ng of Fas39 or p2140 luciferase reporter plasmid using Lipofectamine2000 (Invitrogen Corp., CA, USA). At 24 h after transfection, the cells were exposed to hypoxia (0.3%) or maintained at normoxia for another 24 h. Luciferase activity was assessed with the Luciferase Reporter Assay system (Promega, WI, USA) and a luminometer (Berthold Technologies, TN, USA) according to the manufacturer's instructions.
Flow cytometry
Samples were analyzed on a fluorescence-activated cell sorter (FACSCalibur flow cytometer, Becton Dickinson, San Jose, CA, USA). For propidium iodide staining and analysis of DNA fragmentation, HCT116 cells were plated onto six-well plates at a density of 4 × 105 cells/well 1 day before treatment. Cells were exposed to hypoxia (0.3% O2) or maintained at normoxia for another 36 or 48 h. Cells were then fixed and stained with propidium iodide and analyzed on the flow cytometer according to a standard procedure. For Fas/CD95 expression, HCT116 cells were treated as above for 24 h, rinsed with PBS twice and incubated for 5 min in PBS containing 1 mM Versen in order to detach the cells. The cell suspension was then rinsed twice in TBS and incubated for 30 min on ice with the phycoerythrin-conjugated anti-human Fas/CD95 monoclonal antibody. Finally, cells were rinsed twice with TBS, resuspended in 500 μl TBS and analyzed by FACSCalibur. Data were analyzed by Microcal Origin statistical software. Flow cytometry data were analyzed by WinMDI 2.8 software (Scripps Research Institute, CA, USA).
Abbreviations
- EST:
-
expressed sequence tag
- FACS:
-
fluorescence-activated cell sorter
- FBS:
-
fetal bovine serum
- HDF:
-
human diploid fibroblast
- HIF1:
-
hypoxia inducible factor-1
- HRE:
-
HIF1-responsive element
- RT-PCR:
-
reverse transcription-polymerase chain reaction
- UV:
-
ultraviolet light
- wt:
-
wild-type
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Acknowledgements
We thank Thierry Soussi and Vladimir Bykov for valuable advice and reagents, Bert Vogelstein and Anders Zetterberg for providing cells, Katarina Gradin for technical help with hypoxia treatment and Annelie Waldén for microarray production. This work was supported by the Swedish Cancer Society, Karolinska Institute, Ingabritt och Arne Lundberg Foundation, the Swedish Society of Medicine, the Wallenberg Consortium North, the Nilsson-Ehle Foundation (Lund), and the Swedish Research Council.
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Liu, T., Laurell, C., Selivanova, G. et al. Hypoxia induces p53-dependent transactivation and Fas/CD95-dependent apoptosis. Cell Death Differ 14, 411–421 (2007). https://doi.org/10.1038/sj.cdd.4402022
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DOI: https://doi.org/10.1038/sj.cdd.4402022
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