Introduction

Alzheimer’s disease (AD) is an age-related neurological degenerative disorder and is the most common cause of dementia in older subjects. Clinically, AD typically begins with episodic memory impairment, followed by slow progression toward more general impairment in daily activities, such as attention, executive function, language and visuospatial functions, ultimately leading to a loss of independent daily living and causing a heavy toll on the patient and the family. Mild cognitive impairment (MCI), especially amnestic MCI (aMCI), has been considered the prodromal stage of dementia and has a high risk of converting to AD1,2,3,4.

The accumulation of amyloid beta (Aβ) plaques and tau tangles, as early markers for AD, is found widely in the disease progression of aMCI/AD5,6. Additionally, convergence evidence has confirmed AD subjects were associated with gray matter atrophy or volume reduction7 and thinner cortices8,9 based on structural magnetic resonance imaging (MRI). Functional imaging may bridge the finding between early molecular alterations (for example, amyloid beta deposition) and later clinical symptoms (e.g., cognitive changes) or structural changes in AD10. Resting-state functional MRI (fMRI) connectivity is a noninvasive measure that enables the assessment of the temporal correlations in spontaneous brain activity11,12.The correlational structures of these fluctuations among spatially distributed brain regions are used to identify connectivities and networks within the brain and the resting-state networks (RSNs) are characterized by spatially coherent spontaneous activities; they are also used to depict the connectivity patterns that are commonly involved in functions such as the sensory, attention and executive control networks and default mode processing13,14.

The research on large-scale RSNs offers a typical paradigm for investigating the relationship between cognitive dysfunction and brain activity alterations in neurological disorders15,16,17. In terms of AD/MCI, convergence evidence based on fMRI studies showed that functional integration is disturbed and that the functional changes are related to cognitive variables in the resting-state. For example, recent studies have demonstrated that functional connectivity and networks were selectively impaired in individuals with AD or at risk for AD, especially in higher cognitive RSNs such as the default mode network (DMN)18,19,20,21, attention network22,23, executive control network24 and salience network(SAL)25,26. All of these findings support the idea that AD is a disconnection syndrome27,28 and that the selective impairment patterns in the RSNs may characterize the traits and states of AD18. However, most of these studies mainly focused on the connectivity alterations within individual networks or between one or two RSNs, which may limit our understanding of the pathophysiological substrate of AD. Fortunately, researchers began to realize the importance of studying the functional disturbances from an integrative perspective. For example, Wang and colleagues studied the connectivity patterns among ten modes based on a previously defined template and demonstrated that nearly half of the decreased functional connectivity was between the prefrontal and parietal lobes29. Li and colleagues found that the network-to-network connectivity patterns changed among eight cognitive networks and sensory networks in AD patients30 and some network studies have demonstrated the brain’s topologically reorganized architecture in AD and MCI31,32,33,34,35. Importantly, the intra-network and inter-network patterns of the resting-state connections in subjects with AD and autosomal dominant AD and individuals at high risk (family history, APOE-4 allele and amyloid burden) for AD have been well studied and the results have demonstrated that the large-scale brain networks are vulnerable in AD patients and in individuals at high risk of converting to AD25,36,37,38,39,40,41,42.

Inspired by the above studies, we speculated that intra-network functional connectivity would be impaired in AD and MCI in comparison with normal cognitive (NC) healthy volunteers and that the interactions between different RSNs would change simultaneously. We also expected that brain function connectivities would differ based on patient variations in cognitive impairment severity. To test these hypotheses, we investigated the altered functional connectivity patterns at three levels (integrity, network and connectivity) based on five previously defined key RSNs: the default mode network (DMN), dorsal attention network (DAN), control network (CON), salience network (SAL) and sensorimotor network (SMN), based on the fMRI data acquired under no-task conditions from 35 patients with AD, 27 patients with MCI and 27 age-matched NC volunteers. In addition, we also explored the relationships between the Mini Mental State Examination (MMSE) scores and these strengths of functional connectivities at each scale by using Pearson’s correlations after controlling for age and gender effects in the patient groups.

Materials and Methods

Ethics statement

This study was approved by the Medical Ethics Committee of the Chinese PLA General Hospital. All participants were assessed at the Chinese PLA General Hospital and written consent forms were obtained from the subjects or their legal guardians (spouses or children) and the methods were carried out in accordance with the approved guidelines.

A portion of the subjects were included in our previous studies on the perceptual and response interference in AD43 and the changes in regional brain activity44, the functional connectivity patterns of the amygdala45 and the same dataset was used in studying the functional connectivity patterns of the thalamic46 and marginal divisions47 in the resting-state and also the impaired gray matter volume pattern48 based on structural MRI. Here, we provide a brief introduction regarding the data inclusion and exclusion criteria, acquisition and processing to maintain the scientific integrity of the present study. Detailed data descriptions can be found in Part I of the supplemental material.

Subjects

The subjects were recruited from two sources: as outpatients from the Chinese PLA General Hospital or through a website advertisement (http://www.301ad.com.cn, Chinese version). All subjects met the identical methodological stringency criteria and comprehensive clinical details were described in our previous studies; additional details regarding participant selection and exclusion for this data set can be found elsewhere44,45,46,47 and Part I of the supplemental material. Briefly, the recruited AD patients were diagnosed using the National Institute of Neurological and Communicative Disorders and Stroke and the Alzheimer’s Disease and Related Disorders Association criteria for probable AD. The enrolled MCI patients fulfilled the diagnostic criteria described by Petersen et al.2. At the same time, our AD and MCI patients also met the core clinical criteria of the new diagnostic criteria for probable AD dementia and MCI due to AD49,50. Exclusion criteria included significant neurological or psychiatric illness that can influence cognitive functions as well as significant unstable systemic illness or organ failure. In addition, patients with a metallic foreign body were also excluded from the study for security and imaging quality control reasons. No subjects were treated with any medication that could have influenced their cognition during the data collection. Each subject was right-handed and underwent a battery of neuropsychological tests: the Mini-Mental State Examination (MMSE), Auditory Verbal Learning Test (AVLT), Geriatric Depression Scale, Clinical Dementia Rating (CDR) and Activities of Daily Living (ADL) scale.

Briefly, after excluding subjects with large head motions (see the criteria in data preprocessing), 89 subjects—35 AD patients, 27 MCI subjects and 27 age- and gender-matched NC subjects—were included for further analysis. Demographic and neuropsychological details for the subjects are shown in Table 1 and can be found in our previous studies44,45,46,47.

Table 1 Demographic, clinical and neuropsychological data in normal control (NC), mild cognitive impairment (MCI) and Alzheimer’s disease (AD) subjects.
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fMRI data acquisition and preprocessing

As was previous noted, the MRI scans were performed at the Chinese PLA General Hospital, Beijing, China, with a 3.0 T GE MR system (GE Healthcare, USA) using a standard head coil. During the scanning, the subjects were instructed to keep their eyes closed and relax; comfortable foam padding was used to minimize head motion and ear plugs were used to reduce the scanner noise. Before the resting fMRI data were collected, T2-weighted images were collected and evaluated by two senior radiologists. Resting-state fMRI data were acquired using an echo planar imaging (EPI) sequence with repetition time = 2000 ms, echo time = 30 ms, flip angle = 90°, matrix = 64 × 64, field of view = 220 mm × 220 mm, slice thickness = 3 mm and slice gap = 1 mm. Each volume was composed of 30 axial slices and each functional run lasted for 6 minutes and 40 seconds.

The data were preprocessed using the same steps as those in our previous studies using the in-house Brainnetome fMRI toolkit (Brat, www.brainnetome.org/brat) based on statistical parametric mapping (SPM8, http://www.fil.ion.ucl.ac.uk/spm). These steps were: (1) slice-timing with reference slice = 2, (2) realignment to the first volume, (3) normalization to a standard EPI template and reslicing to 2 × 2 × 2 mm cubic voxels, (4) de-noising by regressing out multiple effects, i.e., the six motion parameters, the constant, the linear drift and the mean time series of all voxels within the white matter and cerebrospinal fluid and (5) temporal filtering (0.01–0.08 Hz) to reduce noise. The data were not further smoothed for we intended to investigate the connectivity patterns of the prior defined seed regions. Group differences in head motion followed those in Van Dijk’s study51 and no significant differences in head motion were found among the three groups (Table 1)47.

Connectivity analysis

The five RSNs used in this study have been well investigated in previous studies36,37,38,39,52. To maintain consistency with these prior studies, the seed regions in the present study were defined a prior based on a study by Dr. Ances; specifically, thirty-six spherical (6 mm radius) regions of interest (ROIs) that represented the five RSNs(DMN, DAN, SAL, CON and SMN) were obtained using the Brainnetome fMRI toolkit. As in previous studies36,37,38,39,52, the SMN included the primary auditory, primary visual and somatomotor cortices. The Montreal Neurological Institute coordinates of the 36 ROIs are presented in Table S1 and the individual ROIs that were displayed on the brain surfaces are shown in Figure S1 in the supplemental material.

The representing mean time series was estimated by averaging the time series of all voxels in this ROI. The Pearson’s correlation coefficients were computed between each pair of ROIs for each subject. Fisher’s r-to-z transformation was applied to obtain Z scores and improve the normality of the correlation coefficients. Then, the connectivity pattern of these ROIs was investigated at the following three levels:

  1. 1

    Integrity: for each node, the integrity Z score was defined as the sum of connection strength, that is, , where is the Z score between the ith and jth ROI. This measure is equivalent to “degree centrality” in the graph theory.

  2. 2

    Network: for each of the five RSNs, the intra-network strength was defined as the mean connection strength of the ROIs in the same network, i.e., , where is the number of ROIs within a specific subnetwork X and k is a range from 1 to 5 that represents the five subnetworks. For each pair of subnetworks, the inter-network connectivity strength was defined as the mean strength of all of the possible connections, that is, , where X and Y represent the subnetworks of the five selected RSNs.

  3. 3

    Connectivity: the Z scores of each pair of ROIs among the included 36 seed regions.

Statistical analysis

To assess the statistical significance, the Z scores at each level were entered into one-way analyses of variance (ANOVAs) with group as a factor (3 groups: NC, MCI, AD) after age and gender effects were regressed out using a general linear model; the statistical significance was P < 0.05. Considering that we have performed many times comparisons among the three groups, we performed a 10,000 times random permutations to test if the identified altered connections are really significant. For all the identified impaired connectivity, the significant effects were assessed by post hoc two-sample, two-sided t-tests of NC versus MCI, NC versus AD and MCI versus AD (P < 0.05).

To investigate the relationship between functional connectivity and cognitive ability, we also explored the Pearson’s correlations between the MMSE scores and the Z scores at each level in the MCI, AD and MCI plus AD groups. Because these relationships were exploratory in nature, we used a statistical significance level of P < 0.05 (uncorrected).

Results

Group differences at the three levels

For each group, a 36 × 36 functional connectivity matrix was computed. In the NC group, the majority of strong positive functional connectivities were within each network and most negative correlations were between different networks (Figure S2A–C). A similar pattern was observed in the MCI and AD groups, except that the intra-network and inter-network correlations were decreased (Figure S2D–F).

At the integrity level, significant group differences were anchored in the right motor cortex (rMC), the bilateral primary visual (V1) and the right posterior intraparietal sulcus (rpIPS) (Table 2, Fig. 1). Post hoc analysis showed that the integrity connectivity of these regions was reduced in the AD and MCI groups compared with the NC, but there was no significant alteration between the AD and MCI groups (Fig. 1).

Table 2 Group effects and correlations with MMSE scores at the integrity level.
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Figure 1
figure 1

Group effects by one-way ANOVA for and the relationship between integrity connectivity and MMSE scores.

Bar graphs show the differences in the mean Z scores for the affected regions among the three groups. The changed regions were anchored in the right motor cortex (rMC) (A), the right posterior intraparietal sulcus (rpIPS) (B) and the bilateral primary visual (V1) (C,D). The Z scores for the bilateral V1 and rpIPS were higher in the NC group than in the AD and MCI groups and the rMC of the NC group was greater than that of the MCI group only. The NC group is indicated by black rectangles, the MCI group by grey rectangles and the AD group by white rectangles. The error bars represent the standard error of each subgroup. The scatter plots show the relationship between the mean Z scores of rpIPS and MMSE scores in the MCI patients (r = −0.418, p = 0.03) (E).

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At the network level, the composite Z scores of the DMN, CON and SMN showed significant differences in the severity of the cognitive impairments among the groups (Table 3, Fig. 2A). Compared with those for the NC group, the Z scores within the DMN and SMN showed significant decreases in the AD and MCI groups (Fig. 2A). Particularly, the Z scores for CON showed a slight increase in the MCI group but a sharp decrease in the AD group (Fig. 2A). For the inter-network pairs, only the DAN-SMN connectivity showed a significant decrease in the AD and MCI groups (Table 4, Fig. 3A).

Table 3 Group effects and correlations with MMSE scores within individual RSNs.
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Table 4 Group effects and correlations with MMSE scores on RSN pairs.
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Figure 2
figure 2

(A) The differences in mean Z scores within the five RSNs. The bar graph shows that the majority of network composite Z scores tended to lower value in DMN and SMN with increasing disease severity. Compared with the NC group, the Z scores within the DMN and SMN showed significant decreases in the AD and MCI groups (DMN, p = 0.005 for MCI, p = 0.008 for AD; and SMN, p = 0.01 for MCI, p = 0.006 for AD). A transient increase in composite Z scores was seen in the CON between NC and MCI, but a sharp decrease (MCI versus AD, p = 0.011) occurred in the AD group. The NC group is indicated by black rectangles, the MCI group by grey rectangles and the AD group by white rectangles. The error bars represent the standard error of each subgroup. (B) Scatter plots showing the relationship between the mean Z scores for the DMN (r = −0.291, p = 0.022), the CON (r = −0.359, p = 0.004) and the MMSE scores in the AD and MCI patients. MCI patients are indicated by the blue triangles and the AD patients by the blue circles.

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Figure 3
figure 3

(A) Bar graph of the DAN-SAN connectivity strength in the three groups, which shows that the strength differed significantly in the NC group compared with the MCI (p = 0.008) and AD (p = 0.0258) groups. The NC group is indicated by black rectangles, the MCI group by grey rectangles and the AD group by white rectangles. The error bars represent the standard error of each subgroup. (B) Scatter plots showing the relationship between the mean Z scores for DAN-CON (r = −0.252, p = 0.048) and the MMSE scores in the AD and MCI patients. The MCI patients are indicated by the blue triangles and the AD patients by the blue circles.

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As Fig. 4 shows, a large number of changed intra-network and inter-network functional connectivities were identified by one-way ANOVA after age and gender effects were controlled using linear regress (Fig. 4A; details of the statistical values can be found in Table S2). The most significantly affected pairs were mainly distributed between the DMN and other RSNs and between the DAN and SMN (Fig. 4A). Post hoc analysis strengthened these manifestations (Fig. 4C–E). Specifically, the most significant alterations were mainly distributed between the DAN and the SMN in the MCI subjects in comparison with the NC individuals (Fig. 4C).

Figure 4
figure 4

(A) Distribution of the altered functional connectivity. All affected ROI pairs for all 5 RSNs (blue for DMN, dark green for DAN, yellow for CON, green for SAL and pink for SMN), except for intra-network ROI pairs, were mainly distributed between the DMN and other RSNs and between the DAN and the SMN.(B) The correlations between the functional strength of the affected ROI pairs and the MMSE scores. The blue color represents the functional connectivity that shows positive correlations with the MMSE scores and the red color represents negative correlations. (C) The differences in connectivity between the NC and MCI groups. (D) The differences in connectivity between the NC and AD groups. (E) The differences in connectivity between the MCI and AD groups. In the subfigure (C–E), the blue color indicates that the functional connectivity of the former group is stronger than that for the latter and the red color indicates the reverse. For details, please refer to Table S2 in the supplemental material.

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For all the identified impaired connectivities, random permutation tests indicate that these findings are significant with P < 0.05 (Figs 1, 2, 3, 4. Tables 2, 3, 4, S2).

Correlations between altered connectivity and MMSE scores

At the integrity level, the connectivities of the laPFC and the rpIPS demonstrated significant correlations with the MMSE scores in the MCI patients (Table 2, Fig. 1). At the network level, the affected intra-network interactions between the DMN and CON and the DAN and CON were significantly correlated with the MMSE scores in the AD and MCI patients (Tables 3, 4, Figs 2B and 3B). Our results also demonstrated that 9 connectivity pairs showed positive correlations with MMSE scores and that 4 connectivity pairs showed negative correlations with MMSE scores in the identified impaired connectivity pairs (Fig. 4B; for details, please refer to Table S2).

Discussion

In the present study, widespread impaired functional connectivity patterns including intra-network and inter-network disconnections that could have been the basis for cognitive impairment were identified in the MCI and AD patients. Based on the integrity-level analysis, the differences were anchored in the right motor cortex (rMC), the bilateral primary visual (V1) and the right posterior intraparietal sulcus (rpIPS) in the AD and MCI subjects (Table 2, Fig. 1). The connectivities within the DMN and SMN were significantly decreased in the MCI and AD groups and the connectivities within the CON increased slightly in the MCI group whereas it showed a sharp decrease in the AD group (Fig. 2A). Connectivity analysis of both the inter-network and the ROI pairs showed that the DAN-SMN connectivity was impaired significantly in the patient groups, especially in the MCI individuals. Among the identified impaired connectivities, some demonstrated significant correlations with cognitive ability as assessed by the MMSE scores (Fig. 4B and Table S2).

Impaired intra-network connectivity within the RSNs

Consistent with previous studies, the present study demonstrated that the default mode network (DMN) was one of the most affected network in the AD21 and MCI34 subjects (Fig. 2A,B). The DMN plays a key role in cognitive processes, especially in episodic memory processing53,54 and the impaired performance of episodic memory is one of the core features of a diagnosis of AD6; this could be one of the main reasons that impaired functional connectivity within the DMN has been frequently identified in AD and MCI patients using multiple imaging techniques6,19,36. In addition, amyloid plaques, which have been considered the main pathophysiological process of AD to date, were found to be preferentially deposited in regions of the DMN55 and to cause the impaired resting-state fMRI connectivity40,56,57,58. Based on the DMN’s core role in modulating daily cognition, functional deficits in the DMN might contribute to AD pathology and might be potential biomarkers for distinguishing AD from MCI.

In the present study, the functional connectivity within the CON increased slightly in the MCI group, whereas it decreased sharply in the AD group (Fig. 2A). As was described, the CON is crucial for active maintenance of and manipulating information in working memory and for rule-based problem solving and decision making and it is related to executive function59,60,61. MCI patients showed subtle executive dysfunctions in higher-order activities, such as financial capacity62, but everyday abilities were preserved; hence the increased connectivity within the CON may reflect a coherent compensatory recruitment in MCI patients. Consistent with this, neuroimaging studies have suggested that the increased prefrontal activity (the main region of CON) reflects compensatory strategies used in performing cognitive tasks63,64. Clinically, AD patients lose the ability to manage their daily life activities, which could be because activities such as cooking, shopping and driving are target-based problem-solving tasks that require the participation of the executive control brain regions65 and executive dysfunctions are considered pervasive66. Hence, the decreased connectivity within the CON in AD patients might reflect the foundation of executive dysfunction.

An interesting finding was that our results demonstrated that the functional connectivity within the sensorimotor network (SMN) was impaired in the MCI and AD subjects (Figs 1 and 2A). The SMN, composed of the primary visual, auditory and somatomotor cortices, plays a role in receiving external signals, which could aid in perceiving the world, selecting the relevant information and determining the target; it then conveys the signals to the attention or control systems to induce reasonable responses. For many years, the SMN was usually thought to be relatively stable and was seen as a reference network in studying AD and MCI39,67. Although a number of studies have indicated that the functional changes in the olfaction, hearing, visual and motor systems (the major components of the SMN) might precede the onset of cognitive impairments, worsen as the disease progresses and be strong risk factors for AD68,69,70,71, this impaired functional connectivity, together with the recognition that AD pathology will develop over many years, raises the exciting possibility that declines in specific primary daily functions may be early noninvasive biomarkers for AD. Even more provocatively, treating these daily symptoms may help to delay or treat MCI/AD72. Our results provided additional evidence that the affected SMN should arouse attention not only as a reference stable system in studying AD and MCI.

Impaired inter-network connectivity between RSNs in AD and MCI

Resting-state functional connectivity and network analysis provides a new tool for mapping large-scale function and dysfunction in the brain system12,73.Inter-network connectivity, especially between the DAN and the SMN, was impaired in the AD and MCI groups (Fig. 3A, Fig. 4A,C,D and Table S2) and this phenomenon prompted us to rethink the role of the interactions between the RSNs in understanding AD pathology and clinical performance. The dorsal attention network (DAN) is one of the networks that is associated with cognitive functions and the multiple somatosensory integrations of sensory, motor and cognitive systems provide the signals for the organism to perceive and respond to its environment; that is, deficits in any of these components will lead to impaired function at the clinical level66,72,74. Often, decreased attention and sensory or motor declines are seen as signs of aging; clinically, the performance of these functions is poor in MCI individuals and even worse in AD subjects75,76. Our research prompted us to infer that the impaired connectivity within the SMN or reduced connectivity between the SMN and the DAN might be one of the reasons for the attention deficits in the early stages of AD and MCI. Additionally, convergence evidence has suggested that AD pathology develops over many years, raising the exciting possibility that daily cognitive impairments, even very slight (such as reduced sensory or motor ability) may be early, noninvasive markers for AD72.

In addition to the above-discussed SMN and DAN, the interactions among the CON, SAL and DMN were also impaired in the AD and MCI groups (Fig. 4A,D,E). Previous resting state fMRI studies have demonstrated aberrant functional connectivity of DMN, SAL and CON in AD36,77, aMCI78 and even in normal cognition with amyloid burden40,79. Convergent evidence has suggested that these three brain systems were closely correlated and play particularly crucial roles in higher cognitive function15,80,81. Functional connectivity between the SAL and DMN is important for cognitive control80,82 and the SAL also plays a central role in switching between the CON and the DMN80,82,83,84. Our findings provide additional evidence to support the viewpoint that the CON, SAL and DMN are intrinsically well-organized in normal healthy subjects and that aberrations in these networks are the prominent features of functional deficits in AD16,83,85. By contrast, the DMN plays a core role in brain activity and other networks that are involved in succession, which suggests that AD pathology might spread from the DMN to the nearby networks, including those involved in visuospatial and executive function and in other peripheral networks86. Therefore, we speculated that AD patients who show clinical attention deficits, memory impairment, executive dysfunction and other disabilities might be reflecting the integration dysfunction in the different brain networks31,87.

Further discussion, limitation and future directions

It should be noted that for multiple metrics but the CON (Figs 2, 3, 4), MCI subjects tend to be similar to AD patients (and different from controls), with little differences between MCI and AD patients. This might because the severity of AD individuals were still mild (35 subjects, 25 with CDR = 1, 10 with CDR = 2) and the variability among patients was relatively high46. This might be the reason that we only found some significant correlations between the used measures and cognitive ability in the MCI and AD patients groups (Figs 1, 2, 3 and Table S2). Another possible reason is that the MCI subjects might include different subgroups, for not all the MCI individuals will convert to AD. In fact, for all the used data in the present study, 14 of the 27 MCI subjects were recruited to return for an examination and data collection after around one year (range: 10–16 months), one subject converted to AD47. Hence, a long time longitudinal study is needed for further investigation.

Notably, the impaired connectivity at all three levels (integrity, network and connectivity) were significantly correlated with performances on the Mini Mental State Examination (MMSE) neuropsychological tests (Figs 1, 2B, 3B and 4B). The MMSE involves multiple cognitive domains and reflects global brain function; it can be used as a tool for quantitatively assessing the severity of cognitive impairment and reflects the cognitive changes that occur during the progression of AD88. As discussed in our previous work, the correlations between MMSE and connectivity and network markers are relatively scant because the MMSE is a brief general screening tool34. However, based on the robust findings from previous studies21,36,89, including our own, we speculated that the correlations between MMSE scores and network functional connectivity indicate that abnormal brain function might be a feature that represents disease severity and could potentially be used as an early marker to distinguish patients from healthy subjects.

As is widely known, the whole brain network is complex, varied and interrelated; the five networks in this study are merely a small part of this complex system and thus, a whole-brain network analysis with finely defined regions is needed in the future. Second, cross-sectional research cannot dynamically observe changes in network patterns with disease progression36. Also many studies have found neuronal dysfunction and disconnection of brain network in normal cognition with amyloid burden40,41,42,79 or APOE e4 carrier90,91. Hence, longitudinal studies combining multiple imaging measures (such as, fMRI, structure MRI, PET etc.) and genetic genotype are needed in the future to follow individuals from healthy to disease states and to different severity levels, exploring network-vulnerability interactions and to study different subtypes of AD and MCI.

Conclusion

The novel finding of the present study is the relationship between disease severity and impaired intra-network and inter-network connectivity from global to fine-pair connectivity in AD and MCI patients. The results demonstrated a progressive alteration of network connectivity; at the early stage of the disease (MCI), sensorimotor and attention functions were involved, but with disease progression, the whole-brain function degeneration induced a wider range of inter-network impairments. All of these findings provide new insight into AD pathophysiology and suggest that altered network connectivity patterns may be useful for the preclinical determination of AD.

Additional Information

How to cite this article: Wang, P. et al. Aberrant intra- and inter-network connectivity architectures in Alzheimer,s disease and mild cognitive impairment. Sci. Rep. 5, 14824; doi: 10.1038/srep14824 (2015).