Abstract
THE destructive effects of radiation have been studied for 80 yr. Most techniques involve looking at the surviving cells, which tend to be the more resistant cells of the tissue. On the assumption that the results are representative of all cells in the tissue, many conclusions have been drawn. On the other hand, Cheng and Leblond have used tritiated thymidine (3HTdR) to kill cells synthesising DNA in the crypts of the small intestine1. Two surprising features of their experiments have provoked little comment. First, very low doses (40–50 µCi per mouse) of 3HTdR caused measurable cell killing and second, the killing (evident from the presence of labelled apoptotic-like2 phagosomes1) was not random throughout the crypt but occurred selectively at the crypt base where relatively few cells are in S (refs 3 and 4) and where the stem cells are presumably located1,3–5. I report here that the presence of hypersensitive cells at the base of the crypts can be demonstrated after whole-body X or γ irradiation, and to describe the time sequence for the production and loss of these killed cells together with their dose-response relationship.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Cheng, H. & Leblond, C. P. Am. J. Anat. 141, 537–562 (1974).
Kerr, J. F. R., Wyllie, A. H. & Currie, A. R. Br. J. Cancer 26, 239–257 (1972).
Potten, C. S., Kovacs, L. & Hamilton, E. Cell Tissue Kinet. 7, 271–283 (1974).
Al-Dewachi, H. S., Wright, N. A., Appleton, D. R. & Watson, A. J. Virchows Arch. B Cell Path. 18, 225–243 (1975).
Potten, C. S. Bull. Cancer 62, 419–430 (1975).
Leblond, C. P. & Cheng, H. in Stem Cells of Renewing Cell Populations (ed. Cairnie, A. B., Lala, P. K. & Osmond, D. G.) 7–31 (Academic, New York, 1976).
Potten, C. S. in Stem Cells of Renewing Cell Populations (ed. Cairnie, A. B., Lala, P. K. & Osmond. D. G.) 79–84 (Academic, New York. 1976).
Hendry, J. H. & Potten, C. S. Int. J. radiat. Biol. 25, 583–588 (1974).
Potten, C. S. & Hendry, J. H. Int. J. radiat. Biol. 27, 413–424 (1975).
Withers, H. R. & Elkind, M. M. Int. J. radiat. Biol. 17, 261–267 (1970).
Alper, T. et al. Br. J Radiol. 35, 722–723 (1962).
Oakberg, E. F. Proc. Soc. exp. Biol. Med. 109, 763–767 (1962).
Oakberg, E. F. & Clark, E. J. Cell comp. Physiol. 58, Suppl.1, 173–182 (1961).
Trowell, O. A. Int. J. radiat. Biol. 4, 163–173 (1961).
Ohzu, E. Radiat. Res. 26, 107–113 (1965).
Rugh, R. & Grupp, E. J. exp. Zool. 141, 571–587 (1959).
Snow, M. H. L. J. Emhryol. exp. Morph. 79, 601–615 (1973).
Sinclair, W. K. & Morton, R. A. Radiat. Res. 29, 450–474 (1966).
Steffensen, D. M. Nature 201, 205–206 (1964).
Cairns, J. Nature 255, 197–200 (1975).
Bianchi, M., Baarli, J. & Sullivan, A. H. IAEA-SM-179/6 349–357 (International Atomic Energy Agency, Vienna, 1974).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
POTTEN, C. Extreme sensitivity of some intestinal crypt cells to X and γ irradiation. Nature 269, 518–521 (1977). https://doi.org/10.1038/269518a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/269518a0
This article is cited by
-
A tissue-intrinsic IL-33/EGF circuit promotes epithelial regeneration after intestinal injury
Nature Communications (2023)
-
Clone wars: From molecules to cell competition in intestinal stem cell homeostasis and disease
Experimental & Molecular Medicine (2022)
-
Non-enzymatic role of SOD1 in intestinal stem cell growth
Cell Death & Disease (2022)
-
Therapeutic approach of adipose-derived mesenchymal stem cells in refractory peptic ulcer
Stem Cell Research & Therapy (2021)
-
Interplay among p21Waf1/Cip1, MUSASHI-1 and Krüppel-like factor 4 in activation of Bmi1-CreER reserve intestinal stem cells after gamma radiation-induced injury
Scientific Reports (2020)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.