Abstract
On phagocytosing a microorganism, the neutrophil zpolymor-phonuclear leukocyte, PMN) consumes oxygen at a sharply elevated rate1. The oxygen is used to kill the microorganism, presumably being used to produce a potent oxidizing agent or agents. Candidates for these bactericidal agents are singlet oxygen, hydroxyl radical and chlorinating agents (that is, species containing ‘active’ Cl in a formal +1 oxidation state: HOCl, Cl2, N-chloroamides, and so on)1–5. We now report a semiquantitative assay for PMN-generated active chlorine based on its trapping with 1,3,5-trimethoxybenzene (TMB). Using this assay, we have found that at least 28% of the oxygen consumed by stimulated normal human PMNs is converted to active chlorinating agents.
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References
Klebanoff, S. J. & Clark, R. A. The Neutrophil: Function and Clinical Disorders (North-Holland, Amsterdam, 1978).
Klebanoff, S. J. Ann. int. Med. 93, 480–489 (1980).
Weiss, S. J. & LoBuglio, A. F. Lab. Invest. 47, 5–18 (1982).
Root, R. K. & Cohen, M. S. Rev. infect. Dis. 3, 565–598 (1981).
Gabig, T. G. & Babior, B. M. A. Rev. Med. 32, 313–326 (1981).
Foote, C. S., Abakerli, R. B., Clough, R. L. & Lehrer, R. I. in Bioluminescence and Chemiluminescence (eds DeLuca, M. A. & McElroy, W. D.) 81–88 (Academic, New York, 1981).
Foote, C. S., Abakerli, R. B., Clough, R. L. & Shook, F. C. in Biological and Clinical Aspects of Superoxide and Superoxide Dismutase (eds Bannister, W. H. & Bannister, J. V.) 222–230 (Eisevier, New York, 1980).
Klebanoff, S. J. J. exp. Med. 126, 1063–1078 (1967).
Thomas, E. L. Infect. Immunity 23, 522–531 (1979).
Stelmaszynska, T. & Zgliczynski, J. M. Eur. J. Biochem. 45, 305–312 (1974).
Harrison, J. E. & Schultz, J. J. biol. Chem. 251, 1371–1374 (1976).
Sips, H. J. & Harners, M. N. Infect. Immunity 31, 11–16 (1981).
Albrich, J. M., McCarthy, C. A. & Hurst, J. K. Proc. natn. Acad. Sci. U.S.A. 78, 210–214 (1981).
Weiss, S. J., Klein, R., Slivka, A. & Wei, M. J. clin. Invest. 70, 598–607 (1982).
Castelfranchi, G. & Borra, G. Ann. Chim. (Rome) 43, 293–297 (1953).
Clark-Lewis, J. W. Aust. J. Chem. 10, 505–506 (1957).
Hohn, D. C. & Lehrer, R. I. J. clin. Invest. 55, 707–713 (1975).
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Foote, C., Goyne, T. & Lehrer, R. Assessment of chlorination by human neutrophils. Nature 301, 715–716 (1983). https://doi.org/10.1038/301715a0
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DOI: https://doi.org/10.1038/301715a0
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