Abstract
A putative T-cell receptor gene was isolated from the DNA of the helper hybridoma, 2B4. Analysis of the sequence components of this rearranged gene indicates the presence of separate variable (V), diversity (D) and joining (J) region elements analogous to those of heavy-chain immunoglobulins. These findings further support the belief that the products of this locus are involved in antigen recognition by T cells. There is no evidence of somatic mutation between the putative germ-line and the expressed variable-region gene.
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References
Matzinger, P. Nature 292, 497–501 (1981).
Williamson, A. R. Nature 283, 527–532 (1980).
Kappler, J. W., Skidmore, B., White, J. & Marrack, P. J. exp. Med. 153, 1198–1214 (1981).
Heber-Katz, E. et al. J. exp. Med. 155, 1086–1099 (1982).
Hunig, T. R. & Bevan, M. J. J. exp. Med. 155, 111–125 (1982).
Lonai, P. et al. J. exp. Med. 154, 1910–1921 (1981).
Elliot, B. E., Nagy, Z. A., Ben-Neriah, Y. & Givol, D. Nature 285, 496–498 (1980).
Hedrick, S. M., Cohen, D. I., Nielsen, E. A. & Davis, M. M. Nature 308, 149–153 (1984).
Hedrick, S. M., Nielsen, E. A., Kavaler, J., Cohen, D. I. & Davis, M. M. Nature 308, 153–158 (1984).
Yanagi, Y. et al. Nature 308, 145–149 (1984).
Samelson, L. E., Germain, R. N. & Schwartz, R. H. Proc. natn. Acad. Sci. U.S.A. 80, 6972–6976 (1983).
Allison, J. P., MacIntyre, B. W. & Block, D. J. Immun. 129, 2293–2300 (1982).
Haskins, K. et al. J. exp. Med. 157, 1149–1169 (1983).
Benoist, C. O., Mathis, D. J., Kanter, M. R., Williams, V. F. & McDevitt, H. O. Proc. natn. Acad. Sci. U.S.A. 80, 534–538 (1983).
Huynh, T. V., Young, R. A. & Davis, R. W. in DNA Cloning Techniques: A Practical Approach (ed. Glover, D. M.) (IRL Press, Oxford, 1984).
Kabat, E. A., Wu, T. T., Bilofsky, H., Reid-Miller, M. & Perry, H. in Sequences of Proteins of Immunological Interest (US Dept of Health and Human Services, Washington, DC, 1983).
Maniatis, T. et al. Cell 15, 687–701 (1978).
Viera, J. & Messing, J. Gene 19, 259–268 (1982).
Maxam, A. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).
Parslow, T. G., Blair, D. L., Murphy, W. J. & Granner, D. K. Proc. natn. Acad. Sci. U.S.A. (in the press).
Early, P., Huang, H., Davis, M., Calame, K. & Hood, L. Cell 19, 981–992 (1980).
Sakano, H., Maki, R., Kurosawa, Y., Roeder, W. & Tonegawa, S. Nature 286, 676–682 (1980).
Schilling, J., Clevinger, B., Davie, J. M. & Hood, L. Nature 283, 35–40 (1980).
Kurosawa, Y. & Tonegawa, S. J. exp. Med. 155, 201–218 (1982).
Sakano, H., Kurosawa, Y., Weigart, M. & Tonegawa, S. Nature 290, 562–579 (1981).
Alt, F. W. & Baltimore, D. Proc. natn. Acad. Sci. U.S.A. 79, 4118–4122 (1982).
Hood, L. et al. Cold Spring Harb. Symp. quant. Biol. 45, 887–898 (1981).
Jerne, N. K. Eur. J. Immun. 1, 1–9 (1971).
Kaye, J. & Janeway, C. J. exp. Med. (submitted).
Sakano, H., Huppi, G., Heinrich, G. & Tonegawa, S. Nature 280, 288–294 (1979).
Max, E. E., Seidman, J. G. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 76, 3450–3454 (1979).
Amzel, M., Poljak, R. J., Saul, F., Varga, J. M. & Richards, F. F. Proc. natn. Acad. Sci. U.S.A. 71, 1427–1430 (1974).
Davies, D. R., Padlan, E. A. & Segal, D. A. Rev. Biochem. 44, 629–667 (1975).
Acuto, O. et al. Cell 34, 717–726 (1983).
McIntyre, B. W. & Allison, J. P. Cell 34, 739–745 (1983).
Kappler, J., Kubo, R., Haskins, K., White, J. & Marrack, P. Cell 34, 727–737 (1983).
Marrack, P. et al. J. exp. Med. 158, 1077–1091 (1983).
Swain, S. L. Immun. Rev. 74, 129–142 (1983).
Mullins, J. I., Brody, D. S., Binari, R. C. & Cotter, S. M. Nature (in the press).
Clayberger, C., Dekruyff, R. H., Aisenberg, J. & Cantor, H. J. exp. Med. 157, 1906–1919 (1983).
Fresno, M., Nabel, G., McVay-Bordreau, L., Furthmayer, H. & Cantor, H. J. exp. Med. 153, 1246–1259 (1981).
Gascoigne, N. R. J., Chien, Y.-h., Becker, D., Kavaler, J. & Davis, M. M. Nature (submitted).
Lee, N. E. et al. J. exp. Med. (submitted).
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Chien, Yh., Gascoigne, N., Kavaler, J. et al. Somatic recombination in a murine T-cell receptor gene. Nature 309, 322–326 (1984). https://doi.org/10.1038/309322a0
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DOI: https://doi.org/10.1038/309322a0
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