Abstract
L-glutamic acid (L-Glu) and L-aspartic acid (L-Asp) are considered to be major excitatory amino acid transmitters, causing depolarization and excitation of neurones in the mammalian central nervous system (CNS)1,2. These responses have been thought to be an exclusively neuronal property as the excitatory amino acids either did not affect the potential of electrophysiologically unresponsive glial cells3,4, or when an effect was seen, it was attributed to changes in external [K+] (refs 5, 6). Here we report that L-Glu directly depolarizes immunocytochemically-identified astrocytes in primary culture. L- or D-Asp and kainic acid (KA) also depolarized these cells while none or minimal changes in the resting membrane potentials were found in response to N-methyl-D-aspartate, D-glutamate, taurine, L-glutamine or to the inhibitory amino acids γ-aminobutyric acid (GABA) and glycine. We conclude that the membrane potential of astrocytes can no longer be thought of as being responsive only to K+ and that the electrophysiological effects of excitatory amino acids in situ may not be exclusively a neuronal property.
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References
Watkins, J. C. & Evans, R. H. A. Rev. Pharmac. Tox. 21, 165–204 (1981).
Watkins, J. C. in Glutamate: Transmitter in the Central Nervous System (eds Storm-Mathisen, J. & Johnston, B. W.) 1–24 (Wiley, Ltd., Chichester, 1981).
Krnjevic, K. & Schwartz, S. Expl Brain Res. 3, 306–319 (1967).
Wardell, W. M. Proc. R. Soc. 165, 326–361 (1966).
Hosli, L., Andres, F. & Hosli, E. Neuroscience 4, 1593–1598 (1979).
Hosli, L. & Hosli, E. in Glutamine, Glutamate and GABA in the Central Nervous System (eds Hertz, L., Kvamme, E., McGeer, E. G. & Schousboe, A.) 441–455 (Alan R. Liss, Inc., New York, 1983).
McCarthy, K. D. & Harden, T. K. J. Pharmac. exp. Ther. 216, 183–191 (1981).
Bignami, A., Dahl, D. & Rueger, D. in Advances in Cellular Neurobiology (eds Federoff, S. & Hertz, L.) 285–310 (Academic, London 1980).
Eng, L. F. in Proteins of the Nervous System (eds Bradshaw, R. & Schneider, D.) 85–117 (Raven, New York, 1980).
Kimelberg, H. K. Cell Molec. Neurobiol. 3, 1–16 (1983).
Bowman, C. L., Kimelberg, H. K., Frangakis, M. V., Berwald-Netter, Y. & Edwards, C. J. Neurosci. 4, 1527–1534 (1984).
Schousboe, A., Svenneby, G. & Hertz, L. J. Neurochem. 29, 999–1005 (1977).
Fonnum, F. J. Neurochem. 42, 1–11 (1984).
Kimelberg, H. K. et al. in Head Injury: Basic and Clinical Aspects (eds Grossman, R. & Gildenberg, P. L.) 31–44 (Raven, New York, 1982).
Kimelberg, H. K., Bowman, C. L., Biddlecome, S. & Bourke, R. S. Brain Res. 177, 533–550 (1979).
Thomas, R. C. Physiol. Rev. 52, 563–594 (1972).
Constanti, A. & Galvin, M. Brain Res. 153, 183–187 (1978).
Tang, C.-M. thesis. Univ. Pennsylvania (1982).
Villegas, J. in Dynamic Properties of Glia Cells (eds Schoffeniels, E., Franck, G., Hertz, L. & Tower, D. B.) 207–215 (Pergamon, New York, 1978).
Van Calker, D. & Hamprecht, B. in Advances in Cellular Neurobiology Vol. 1 (eds Federoff, S. & Hertz, L.) 31–67 (Academic, New York, 1980).
Evans, T., McCarthy, K. D. & Harden, T. K. J. Neurochem. 43, 131–138 (1984).
Ebersolt, C., Perez, M. & Bockaert, J. J. Neurosci. Res. 6, 643–652 (1981).
Repke, H. & Maderspach, K. Brain Res. 232, 206–211 (1982).
Trimmer, P. A., Evans, T., McCardy, M. S. & Harden, T. K. J. Neurosci. 4, 1598–1606 (1984).
Hosli, L., Hosli, E., Zehntner, C., Lehmann, R. & Lutz, T. W. Neuroscience 7, 2867–2872 (1982).
Hirata, H., Slater, N. T. & Kimelberg, H. K. Brain Res. 270, 358–362 (1983).
Kuffler, S. W., Nicholls, J. G. & Orkand, R. K. J. Neurophysiol. 29, 768–787 (1966).
Kimelberg, H. K., Stieg, P. E. & Mazurkiewicz, J. J. Neurochem. 39, 734–742 (1982).
Frangakis, M. V. & Kimelberg, H. K. Neurochem. Res. 9, 1685–1694 (1984).
Banker, G. A. & Cowan, W. M. Brain Res. 126, 397–425 (1977).
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Bowman, C., Kimelberg, H. Excitatory amino acids directly depolarize rat brain astrocytes in primary culture. Nature 311, 656–659 (1984). https://doi.org/10.1038/311656a0
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DOI: https://doi.org/10.1038/311656a0
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