Abstract
Mammalian cells obtain cholesterol for membrane synthesis mostly via the receptor-mediated endocytosis of low-density lipoprotein (LDL)1. Macrophages and vascular endothelium additionally have receptors that recognize certain modified forms of LDL (for example, acetyl-LDL)2,3. The process by which cholesterol returns from peripheral cells to hepatocytes (reverse cholesterol transport) has not been established; although tissue culture studies have favoured high-density lipoprotein (HDL) as the principal vehicle4,5, the in vivo evidence for this is meagre. When cholesterol-loaded macrophages are incubated in medium containing plasma, cholesterol moves from the cells to HDL and is then esterified by lecithin/cholesterol acyltransferase6. The accumulation of cholesteryl esters in the particles increases their size and decreases their density; enrichment with apoprotein E (apo E) also occurs, producing a decrease in electrophoretic mobility7,8. We now report that similar changes occur in the circulating HDL of rabbits, when their peripheral tissues are loaded with cholesterol by intravenous (i.v.) injection of acetylated or native human LDL. This result suggests that HDL is involved in reverse cholesterol transport in vivo.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Goldstein, J. L. & Brown, M. S. A. Rev. Biochem. 46, 897–930 (1977).
Brown, M. S. & Goldstein, J. L. A. Rev. Biochem. 52, 223–261 (1983).
Stein, O. & Stein, Y. Biochim. biophys. Acta 620, 631–635 (1980).
Glomset, J. A. Am. J. clin. Nutr. 23, 1129–1136 (1972).
Nicoll, A., Miller, N. E. & Lewis, B. Adv. Lipid Res. 17, 53–106 (1980).
Ho, Y. K., Brown, M. S. & Goldstein, J. L. J. Lipid Res. 21, 391–398 (1980).
Basu, S. K., Goldstein, J. L. & Brown, M. S. Science 219, 871–873 (1983).
Gordon, V., Innerarity, T. L. & Mahley, R. W. J. biol. Chem. 258, 6202–6212 (1983).
Havel, R. J., Eder, H. A. & Bragdon, J. H. J. clin. Invest. 34, 1345–1354 (1954).
Goldstein, J. L., Ho, Y. K., Basu, S. K. & Brown, M. S. Proc. natn. Acad. Sci. U.S.A. 76, 333–337 (1979).
Warnick, G. R. & Albers, J. J. High Density Lipoprotein Methodology Workshop (ed. Lippel, K.) 53–69 (NIH publication no. 79-1661, Bethesda, Maryland, 1979).
Lowry, O. H., Rosebrough, N. J. & Randall, R. J. J. biol. Chem. 193, 265–275 (1951).
Miettinen, T. A., Ahrens, E. H. & Grundy, S. M. J. Lipid Res. 6, 411–424 (1965).
Grundy, S. M., Ahrens, E. H. & Miettinen, T. A. J. Lipid Res. 6, 397–410 (1965).
Slater, H. R., Packard, C. J. & Shepherd, J. J. J. biol. Chem. 257, 307–310 (1982).
Van Berkel, T. J. C., Nagelkerke, J. F., Harkes, L. & Kruijt, J. K. Biochem. J. 208, 493–503 (1982).
Mahley, R. W. & Innerarity, T. L. Biochim. biophys. Acta 737, 197–222 (1983).
Hass, A. J., Davis, H. R., Elner, V. M. & Glagov, S. J. Histochem. Cytochem. 31, 1136–1138 (1983).
Blomhoff, R. et al. J. biol. Chem. 259, 8898–8903 (1984).
Slater, H. R., Packard, C. J., Bicker, S. & Shepherd, J. J. biol. Chem. 255, 10210–10213 (1980).
Koelz, H. R., Sherrill, B. C., Turley, S. D. & Dietschy, J. M. J. biol. Chem. 257, 8061–8072 (1982).
Carew, T. E., Pittman, R. C. & Steinberg, D. J. biol. Chem. 257, 8001–8008 (1982).
Mahley, R. W., Weisgraber, K. H., Bersot, T. P. & Innerarity, T. L. High Density Lipoproteins and Atherosclerosis (eds Gotto, A. M., Miller, N. E. & Oliver, M. F.) 149–176 (Elsevier, Amsterdam, 1978).
Daerr, W. H., Gianturco, S. M., Patsch, J. R., Smith, L. C. & Gotto, A. M. Biochim. biophys. Acta 619, 287–301 (1980).
Sherrill, B. C., Innerarity, T. L. & Mahley, R. W. J. biol Chem. 255, 1804–1807 (1980).
Driscoll, D. M. & Getz, G. S. Arteriosclerosis 3, 513a (1983).
Miller, G. J. & Miller, N. E. Lancet i, 16–19 (1975).
Miller, N. E., Forde, O. H., Thelle, D. S. & Mjos, O. D. Lancet i, 965–967 (1977).
Gordon, T., Castelli, W. P., Hjortland, M. C., Kannel, W. B. & Dawber, T. R. Am. J. Med. 62, 707–714 (1977).
Nestel, P. J. & Miller, N. E. High Density Lipoproteins and Atherosclerosis (eds Gotto, A. M., Miller, N. E. & Oliver, M. F.) 51–54 (Elsevier, Amsterdam, 1978).
Reichl, D., Myant, N. B., Rudra, D. N. & Pflug, J. J. Atherosclerosis 37, 489–495 (1980).
Reichl, D., Rudra, D. N., Myant, N. B. & Pflug, J. J. Atherosclerosis 44, 73–84 (1982).
Dory, L., Sloop, C. H., Boquet, L. M., Hamilton, R. L. & Roheim, P. S. Proc. natn. Acad. Sci. U.S.A. 80, 3489–3493 (1983).
Aulinskas, T. H., Van der Westhuyzen, D. R., Bierman, E. L., Gevers, W. & Coetzee, G. A. Biochim. biophys. Acta 664, 255–265 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Miller, N., Ville, A. & Crook, D. Direct evidence that reverse cholesterol transport is mediated by high-density lipoprotein in rabbit. Nature 314, 109–111 (1985). https://doi.org/10.1038/314109a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/314109a0
This article is cited by
-
Nannochloropsis sp. ethanol extract prevents macrophage and LDL oxidation and enhances PON1 activity through the principal active compound lyso-diacylglyceryltrimethylhomoserine (lyso-DGTS)
Journal of Applied Phycology (2018)
-
The Anabolic Effect of Teriparatide is Undermined by Low Levels of High-Density Lipoprotein Cholesterol
Calcified Tissue International (2014)
-
High throughput transcriptome analysis of lipid metabolism in Syrian hamster liver in absence of an annotated genome
BMC Genomics (2013)
-
Antihypercholesterolemic and antioxidative effects of an extract of the oyster mushroom, Pleurotus ostreatus, and its major constituent, chrysin, in Triton WR-1339-induced hypercholesterolemic rats
Journal of Physiology and Biochemistry (2013)
-
Reduction of dietary saturated fatty acids correlates with increased plasma lecithin cholesterol acyltransferase activity in humans
European Journal of Clinical Nutrition (2004)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.