Abstract
Mouse erythroleukaemia cells (also called Friend cells) can be isolated from the spleen of certain strains of mice that have been infected with the Friend virus complex1. The cells resemble pro-erythroblasts and, when exposed to dimethyl sulphoxide (DMSO) or a variety of other chemicals, can be induced to undergo a programme of differentiation which closely resembles the final stages of normal erythropoiesis2. This includes the cessation of proliferation and large increases in the production of messenger RNA for both α- and β-globin. In addition, DMSO induces a rapid (<2h) decrease in c-myc mRNA levels3,4. The c-myc oncogene is expressed in the majority of proliferating normal cells5 and altered expression of the gene has been implicated in the genesis of a wide variety of tumours6–9. To study the influence of oncogene activation on differentiation, we have transfected viral-promoter-driven c-myc genes into mouse erythroleukaemia cells. Constitutive c-myc expression was found to block DMSO-induced differentiation.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Marks, P. A. & Rifkind, R. A. A. Rev. Biochem. 47, 419–448 (1978).
Friend, C. et al. Proc. natn. Acad. Sci. U.S.A. 68, 378–382 (1971).
Lachman, H. M. & Skoultchi, A. I. Nature 310, 592–594 (1984).
Lachman, H. M. et al. Proc. natn. Acad. Sci. U.S.A. 82, 5323–5327 (1985).
Gonda, T. J. et al. Molec. cell. Biol. 2, 617–624 (1982).
Shen-Ong, G. L. C. et al. Cell 31, 443–452 (1982).
Taub, R. et al. Proc. natn. Acad. Sci. U.S.A. 79, 7837–7841 (1982).
Little, C. D. et al. Nature 306, 194–196 (1983).
Corcoran, L. M. et al. Cell 37, 113–122 (1984).
Spandidos, D. A. & Paul, J. EMBO J. 1, 15–20 (1982).
Keath, E. J. et al. Cell 39, 339–348 (1984).
Land, H. et al. Nature 304, 596–602 (1983).
Graves, B. J. et al. Molec. cell. Biol. 5, 1948–1958 (1985).
Ganguly, S. & Skoultchi, A. I. J. biol. Chem. 260, 12167–12173 (1985).
Harrison, P. R. et al. Cell 14, 61–70 (1978).
Gusella, J. et al. Cell 9, 221–229 (1976).
Rabbitts, P. H. et al. EMBO J. 4, 2009–2015 (1985).
Hann, S. R. & Eisenman, R. N. Molec. cell. Biol. 4, 2486–2497 (1984).
Levenson, R. & Housman, D. J. Cell Biol. 82, 715–725 (1979).
Levenson, R. & Housman, D. Cell 25, 5–6 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Coppola, J., Cole, M. Constitutive c-myc oncogene expression blocks mouse erythroleukaemia cell differentiation but not commitment. Nature 320, 760–763 (1986). https://doi.org/10.1038/320760a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/320760a0
This article is cited by
-
MYC in liver cancer: mechanisms and targeted therapy opportunities
Oncogene (2023)
-
RFX1: a promising therapeutic arsenal against cancer
Cancer Cell International (2021)
-
Lyn, a tyrosine kinase closely linked to the differentiation status of primary acute myeloid leukemia blasts, associates with negative regulation of all-trans retinoic acid (ATRA) and dihydroxyvitamin D3 (VD3)-induced HL-60 cells differentiation
Cancer Cell International (2016)
-
MYC oncogene in myeloid neoplasias
Clinical and Translational Oncology (2013)
-
Deciphering c-MYC-regulated genes in two distinct tissues
BMC Genomics (2011)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
