Abstract
Expression of the middle-T antigen of polyomavirus is sufficient to induce transformation of fibroblasts in culture and tumour formation in whole animals1–4. Middle-T can form a complex with the cellular src gene product (p60c-src) and can be phosphorylated by p60c-src in vitro5,6. Studies using middle-T mutants have suggested that the association of middle-T with pc-src may be necessary but not sufficient for transformation7–9. Therefore, we addressed the possibility that middle-T could interact with other tyrosine protein kinases structurally related to p60c-src. Using antibody raised against a fusion protein between β-galactosidase and amino-terminal sequences of p90gag-yes from Y73 virus (anti-yes antibody)10, we have found that middle-T can associate with and be phosphorylated by the c-yes proto-oncogene product, a protein of relative molecular mass (Mr) 62,000 (62K). This raises the possibility that the middle-T–c-yes complex contributes to transformation by polyomavirus.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Asselin, C., Gelinas, C. & Bastin, M. Molec. cell Biol. 3, 1451–1459 (1983).
Kaplan, P. L., Simon, S. & Eckhart, W. J. Virol. 48, 1023–1026 (1985).
Kornbluth, S., Cross, F. R., Harbison, M. & Hanafusa, H. Molec. cell Biol. 6, 1545–1551 (1986).
Treisman, T., Novak, U., Favalaro, J. & Kamen, R. Nature 292, 595–600 (1981).
Bolen, J. B. et al. Cell 38, 767–777 (1984).
Courtneidge, S. & Smith, A. E. Nature 303, 435–439 (1983).
Cartwright, C. A., Hutchinson, M. A. & Eckhart, W. Molec. cell Biol. 5, 2647–2652 (1985).
Courtneidge, S. A. & Smith, A. E. EMBO J. 3, 585–591 (1984).
Markland, W., Cheng, S. H., Oostra, B. A. & Smith, A. E. J. Virol. 59, 82–89 (1986).
Sudol, M. S. & Hanafusa, H. Molec. cell Biol. 6, 2839–2846 (1986).
Ghysdael, J., Nell, J. C. & Vogt, P. K. Proc. natn. Acad. Sci. U.S.A. 78, 2611–2615 (1981).
Shibuya, M., Hanafusa, H. & Balduzzi, P. L. J. Virol. 42, 143–152 (1982).
M. Yoshida, Kawai, S. & Toyoshima, K., Nature 287, 653–654 (1980).
Kawai, S. et al. Proc. natn. Acad. Sci. U.S.A. 77, 6199–6203 (1980).
Kitamura, N., Kitamura, A., Toyoshima, K., Hirayama, Y. & Yoshida, M. Nature 297, 205–208 (1982).
Schaffhausen, B. & Benjamin, T. J. Virol. 40, 184–196 (1981).
Cartwright, C. A., Kaplan, P. L., Cooper, J. A., Hunter, T. & Eckhart, W. Molec. cell Biol. 6, 1562–1570 (1986).
Cooper, J. A., Gould, K. L., Cartwright, C. A. & Hunter, T. Science 231, 1431–1434 (1986).
Hanafusa, H. Proc. natn. Acad. Sci. U.S.A. 63, 318–325 (1969).
Lipsich, L. A., Lewis, A. J. & Brugge, J. S. J. Virol. 48, 352–360 (1983).
Cooper, J. A., Esch, F. S., Taylor, S. S. & Hunter, T. J. biol. Chem. 259, 7841–7853 (1984).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kornbluth, S., Sudol, M. & Hanafusa, H. Association of the polyomavirus middle-T antigen with c-yes protein. Nature 325, 171–173 (1987). https://doi.org/10.1038/325171a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/325171a0
This article is cited by
-
Cell transformation by the middle T-antigen of polyoma virus
Oncogene (2001)
-
Tyrosine kinase signalling in breast cancer: Tyrosine kinase-mediated signal transduction in transgenic mouse models of human breast cancer
Breast Cancer Research (2000)
-
Signal transduction in mammary tumorigenesis: a transgenic perspective
Oncogene (2000)
-
Association between src-kinases and the polyoma virus oncogene middle T-antigen requires PP2A and a specific sequence motif
Oncogene (1999)
-
Medullary thyroid carcinomas in transgenic mice expressing a Polyoma carboxyl-terminal truncated middle-T and wild type small-T antigens
Oncogene (1999)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
