Abstract
E-cadherin is a cell surface glycoprotein responsible for Ca2+-dependent intercellular adhesion between epithelial cells1; it is also called uvomorulin2, L-CAM (ref. 3), cell-CAM 120/80 (ref. 4) or Arc-1 (ref. 5). Because blocking the action of E-cadherin by monoclonal antibodies causes dispersion of compact cell colonies1, this molecule is thought to be an important factor for maintenance of multicellular systems. To demonstrate directly that E-cadherin is involved in cell–cell adhesion, we cloned full-length cDNA encoding E-cadherin from F9 cells and introduced it into L fibroblasts deficient in E-cadherin. These L cells acquire strong Ca2+-dependent aggregating activity by expressing the E-cadherin derived from the introduced cDNA, and were morphologically transformed so as to form colonies in which cells were tightly connected to each other.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
1. Yoshuia-Noro. C, Si,/uki. N. &. Takeichi, M. Devl Diol 101, l^-?7 (1984). 2. Hyal, P., Murdlo, D, Ba">ir;ei, G, & Jacob. F. Cell 21. 927-934 (1980). 3. Galim. W .!.. hdo!m;m, G M <y O.-iminpham.. B. A Pr<.". nain. Acad. Sci. U.S.A. 80, 10/S- !04J i >;>H3<, 4. Damsky. I.. H,. Kitha, J.. Seller, D., Knudsen. K. & Buck, (.. A Cell 34, 455-466 (1983). 5. hnbof. B. A... \oihcrs. H I'.. < ioodman, S. 1'.. & Bin.hrnoicr. W. Cell 35, 667-675 (1983). 6. Peyn.-r.-x N.; Umvard, O. % Jacnb, F Pro, n.-itr.. Acud. Sa. U.S.A. 82, 8067-8071 (1985). 7. Shirayoshi, Y, Nose. A., lw;"aki, K. & Takeichi. M. Cell Struct. Funct. 11, 245-252 (1986). 8. Shirajoshi, Y. ct al. FMBO J 5, 24*5-2488 (1986). 9. Kozak. M. Nuclin' Acid'- Ka.. *2. Sf>7 -H'72 H9-S4). 10. Yon Heijne, G. /. mkf. fiio!. 184, 99-10? (1985). 11 .Wickens, M. > i Mephor. P. SWUM 226, !M5-!05l iWHAt. 12. Gallin, W. J. Sofkin, B. C.. fldelnan. G. M & Cunningha^. B. A. Pntc. natn. Acad. Sci. U.S.A. 84, -SOS. 2812 (H'8/^. 13.Nose, A. A: Takvivhi, M. J. Cell B\"l. 103. 2649.-205X H986). 14.Urushihara, H., O-^ki. H. S. k Takeichi, M. Derl Bio!. 70/206-216 (1979). 15.Sander, F., Nickleii. S. A C'oulson A, R Proc. natn. Acad Sa. U.S.A. 74. 5463-5467 (1977). 16. Xie, H. Cell Struct. Funtt. 8, 315-325 U9H4). Gorman. 17. C. V\:A C.'onwg: a Pineal Approach Vol. M. (ed. Glover, D. M.) 143-190 (1RL, Oxford, 19S1), 18.Southern, P. J. <1 flcr", P. ./. m,y/".v appl (tenet. 1. 327 341 (1982). 19. Nicolas, J. F. & Bery. P Terai^carcinnma Stem Ccih (cds Silver, L. M, Martin, G. R. & Strickiand, S.) 46'-)".4s.^ ".old Sprnit, Harbor Laboratory, New York, 1983). 20.Mjrmuis, T, Fius-.h, i:,. F. <k Sarnbrnok, J. Molecular Cloning, a Laboratory Manual (Cold Spr-np Harbor [,ahor;!U'r>, N,-w Vork. 1982;.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nagafuchi, A., Shirayoshi, Y., Okazaki, K. et al. Transformation of cell adhesion properties by exogenously introduced E-cadherin cDNA. Nature 329, 341–343 (1987). https://doi.org/10.1038/329341a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/329341a0
This article is cited by
-
Live imaging-based assay for visualising species-specific interactions in gamete adhesion molecules
Scientific Reports (2022)
-
Linking E-cadherin mechanotransduction to cell metabolism through force-mediated activation of AMPK
Nature Cell Biology (2017)
-
Congenital immature pure erythroid leukemia with E-cadherin expression
International Journal of Hematology (2017)
-
Alcoholic extracts of Epilobium, Urtica dioica and Evernia prunastri with 5-fluorouracil in controlling murine colon carcinoma cell growth in vitro
Oriental Pharmacy and Experimental Medicine (2017)
-
The number of polyploid giant cancer cells and epithelial-mesenchymal transition-related proteins are associated with invasion and metastasis in human breast cancer
Journal of Experimental & Clinical Cancer Research (2015)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.