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Containerless vitrification of mammalian oocytes and embryos

Nature Biotechnology volume 17, pages 1234–1236 (1999)Cite this article

Abstract

Adapting a proven method for flash-cooling protein crystals to the cryopreservation of live cells.

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Figure 1: Containerless vitrification and warming of embryos followed by normal development.

References

  1. Leibo, S.P. et al. Exp. Cell. Res. 89, 79–88 (1974).

    Article  CAS  Google Scholar 

  2. Leibo, S.P. et al. Cryobiology 15, 257–271 (1978).

    Article  CAS  Google Scholar 

  3. Rall, W.F. Cryobiology 24, 387–402 (1987).

    Article  CAS  Google Scholar 

  4. Menezo, Y. & Veiga, A. in In vitro fertilization and assisted reproduction. (eds Gomel, V & Leung, P.C.K.) 49– 53 (Monduzzi editore, Spain; 1997).

    Google Scholar 

  5. Martino, A. et al. Mol. Reprod. Dev. 45, 503–512 (1996).

    Article  CAS  Google Scholar 

  6. Martino, A. et al. Biol. Reprod. 54, 1059–1069 (1996).

    Article  CAS  Google Scholar 

  7. Rall, W.F. & Fahy, G.M. Nature 313, 573–575 (1985).

    Article  CAS  Google Scholar 

  8. Vajta, G. et al. Cryo-Letters 18, 191–195 (1997).

    Google Scholar 

  9. Vatja, G. et al. Mol. Reprod. Dev. 51, 53–58 (1998).

    Article  Google Scholar 

  10. Teng T-Y. J. Appl. Crystallogr. 23, 387–391 (1990).

    Article  CAS  Google Scholar 

  11. Rodgers, R.W. Methods Enzymol. 276, 183–203 (1997).

    Article  CAS  Google Scholar 

  12. Parkin, S. & Hope, H. J. Appl. Crystallogr. 31 , 945–953 (1998).

    Article  CAS  Google Scholar 

  13. Forest, K.T. & Lane, M.T. US 60/104,266 (1998).

  14. Lane, M. et al. Fertil. Steril. In press (1999).

  15. Lane, M. et al. Mol. Reprod. Dev. 50, 443–450 (1998).

    Article  CAS  Google Scholar 

  16. Gardner, D.K. et al. Hum. Reprod. 13, 3434–3440 (1998).

    Article  CAS  Google Scholar 

  17. Krisher, R.L. et al. Biol. Reprod. 60, 1345–1352 (1999).

    Article  CAS  Google Scholar 

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Acknowledgements

The authors are indebted to Mark Lane for help with establishing the OPS technique and to Tenneille Ludwig for performing the embryo transfers. This research was supported by the National Cooperative Program on Non-Human In Vitro Fertilization and Preimplantation Embryo Development of the National Institute of Child Health and Human Development (NICHD) through grant HD22023. A US patent covering this technology has been applied for (Forest and Lane, #60/104,266).

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Author notes

  1. Michelle Lane

    Present address: Colorado Center for Reproductive Medicine, 799 East Hampden Avenue, Suite 300, Englewood, CO, 80110

Authors and Affiliations

  1. Departments of animal health and biomedical sciences, University of Wisconsin, Madison, 53706, WI

    Michelle Lane, Barry D. Bavister & Elizabeth A. Lyons

  2. Bacteriology, University of Wisconsin, Madison, 53706, WI

    Katrina T. Forest

Authors
  1. Michelle Lane
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  2. Barry D. Bavister
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  3. Elizabeth A. Lyons
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  4. Katrina T. Forest
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Lane, M., Bavister, B., Lyons, E. et al. Containerless vitrification of mammalian oocytes and embryos. Nat Biotechnol 17, 1234–1236 (1999). https://doi.org/10.1038/70795

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