Abstract
A panel of Fabs that neutralize anthrax toxin in vitro was selected from libraries generated from human donors vaccinated against anthrax. At least two of these antibodies protect rats from anthrax intoxication in vivo. Fabs 83K7C and 63L1D bind with subnanomolar affinity to protective antigen (PA) 63, and Fab 63L1D neutralizes toxin substoichiometrically, inhibits lethal factor (LF) interaction with PA63 and binds to a conformational epitope formed by PA63.
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Acknowledgements
Our special thanks go to the military personnel who voluntarily donated blood and bone marrow to this effort. We thank Arthur Friedlander for his gift of PA83 antigen, Janette Delgadillo for expert assistance, and David Myszka for timely Biacore analyses. The views expressed in this article are those of the authors and do not reflect the official policies or positions of the Departments of the Army, Navy, Defense or the US government.
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Wild, M., Xin, H., Maruyama, T. et al. Human antibodies from immunized donors are protective against anthrax toxin in vivo. Nat Biotechnol 21, 1305–1306 (2003). https://doi.org/10.1038/nbt891
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DOI: https://doi.org/10.1038/nbt891
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