Conditional gene targeting based on excision or inversion of loxP-flanked DNA segments by Cre recombinase is a powerful technology for the analysis of gene function, but unexpected expression patterns of cre transgenes, variability of recombination efficiency depending on the target gene and potential toxicity of Cre recombinase represent serious challenges for the experimenter.
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Acknowledgements
We thank H. Liu, Y. Shi, E. Derudder, S. Raffel and S. Casola for allowing us to show unpublished data; S. Casola and other members of the laboratory for discussions of these matters over the years; and S. Casola, C. Wilson and F.T. Wunderlich for comments on this paper. Supported by the National Institutes of Health (K.R.) and the European Union (K.R.).
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Schmidt-Supprian, M., Rajewsky, K. Vagaries of conditional gene targeting. Nat Immunol 8, 665–668 (2007). https://doi.org/10.1038/ni0707-665
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DOI: https://doi.org/10.1038/ni0707-665
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