Key Points
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Cell architecture is dictated by the systems that control the size, number, position and shape of individual organelles.
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Organelle size can be controlled by molecular rulers, quantal synthesis of precursors or dynamic balance-point mechanisms.
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Organelle number is controlled by placing the rate of organelle production or loss under control of organelle number.
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Organelle position is controlled by self-organizing cell polarity systems that orientate the cytoskeleton, which in turn move organelles to defined positions.
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Organelle shape, for membrane-bound organelles, is determined in part by curvature changes driven by lipid composition and lipid-binding proteins, along with active forces exerted on the organelle surface by the cytoskeleton.
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Cells are dynamic systems whose current organization reflects previous organization to an extent that structural variations can be propagated through cell division.
Abstract
The astounding structural complexity of a cell arises from the action of a relatively small number of genes, raising the question of how this complexity is achieved. Self-organizing processes combined with simple physical constraints seem to have key roles in controlling organelle size, number, shape and position, and these factors then combine to produce the overall cell architecture. By examining how these parameters are controlled in specific cell biological examples we can identify a handful of simple design principles that seem to underlie cellular architecture and assembly.
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Acknowledgements
W.F.M. acknowledges the support of the WM Keck Foundation, the Searle Scholars Program and NIH grant R01 GM077004. S.M.R. acknowledges the support of the Sandler Postdoctoral Research Fellowship.
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Glossary
- Self-organizing process
-
A process by which a set of components that can, in principle, be connected in various possible patterns will spontaneously associate into a limited subset of patterns, without any external input of information. 'Self-organization' is to be contrasted with 'self-assembly', in which components can only fit together such that only one pattern is possible.
- Cilium
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A microtubule-based motile and sensory organelle that projects from the surface of many eukaryotic cells.
- Flagellum
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An alternative term for cilia when applied to eukaryotic cells.
- Mitotic spindle
-
A highly dynamic array of microtubules that forms during mitosis and serves to move the duplicated chromosomes apart.
- Centriole
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A short, barrel-like array of microtubules that organizes the centrosome and contributes to cytokinesis and cell-cycle progression.
- Autophagy
-
A pathway for the recycling of cellular contents, in which materials inside the cell are packaged into vesicles and are then targeted to the vacuole or lysosome for bulk turnover.
- Binomial statistics
-
A statistical distribution that describes the probability distribution that is obtained by several successive decisions, each of which has two possible outcomes with constant probabilities. For example, the distribution of the numbers of heads or tails after a particular number of coin flips.
- Fluctuating asymmetry
-
Asymmetry resulting from slight stochastic differences in the molecular concentration in two different regions of a cell.
- Centrosome
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An organelle that contains the centrioles and that anchors the 'minus' ends of microtubules.
- Dynamic morphology
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A term that considers complex organelles not as static structures, but as encompassing the effects of constant shape-altering dynamics.
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Rafelski, S., Marshall, W. Building the cell: design principles of cellular architecture. Nat Rev Mol Cell Biol 9, 593–602 (2008). https://doi.org/10.1038/nrm2460
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DOI: https://doi.org/10.1038/nrm2460
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