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Sgk1 enhances RANBP1 transcript levels and decreases taxol sensitivity in RKO colon carcinoma cells

Oncogene volume 32, pages 4572–4578 (2013)Cite this article

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Abstract

The serum- and glucocorticoid-regulated kinase (Sgk1) is essential for hormonal regulation of epithelial sodium channel-mediated sodium transport and is involved in the transduction of growth factor-dependent cell survival and proliferation signals. Growing evidence now points to Sgk1 as a key element in the development and/or progression of human cancer. To gain insight into the mechanisms through which Sgk1 regulates cell proliferation, we adopted a proteomic approach to identify up- or downregulated proteins after Sgk1-specific RNA silencing. Among several proteins, the abundance of which was found to be up- or downregulated upon Sgk1 silencing, we focused our attention of RAN-binding protein 1 (RANBP1), a major effector of the GTPase RAN. We report that Sgk1-dependent regulation of RANBP1 has functional consequences on both mitotic microtubule activity and taxol sensitivity of cancer cells.

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Acknowledgements

This work was supported in part by the Italian Association for Cancer Research (AIRC grant IG10164 to PL). RA was supported in part by INAIL, CZ.

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Author notes

  1. R Amato and D Scumaci: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Human Health, University Magna Graecia at Catanzaro, Campus S Venuta, Località Germaneto Viale Europa, Catanzaro, Italy

    R Amato, L D'Antona, M Menniti & N Perrotti

  2. Department of Experimental and Clinical Medicine, University Magna Graecia at Catanzaro, Campus S Venuta, Località Germaneto Viale Europa, Catanzaro, Italy

    D Scumaci, R Iuliano, M Di Sanzo, M C Faniello, V Agosti, F S Costanzo & G Cuda

  3. Unit of Medical Genetics and Pathology University Hospital, Policlinico Mater Domini, Catanzaro, Italy

    R Iuliano, E Colao, P Malatesta & N Perrotti

  4. Multiple Myeloma Section, Metabolism Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD, USA

    A Zingone

  5. Department for the Development of Therapeutic Programs, National Cancer Institute ‘Regina Elena’, Rome, Italy

    A M Mileo & M G Paggi

  6. Department of Physiology, University of Tuebingen, Tübingen, Germany

    F Lang

  7. IBPM Institute of Molecular Biology and Pathology, CNR National Research Council, c/o Sapienza University of Rome, Rome, Italy

    P Lavia

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Corresponding authors

Correspondence to P Lavia or N Perrotti.

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Amato, R., Scumaci, D., D'Antona, L. et al. Sgk1 enhances RANBP1 transcript levels and decreases taxol sensitivity in RKO colon carcinoma cells. Oncogene 32, 4572–4578 (2013). https://doi.org/10.1038/onc.2012.470

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