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Cloning and characterization of mouse IL-22 binding protein

Genes & Immunity volume 4, pages 204–211 (2003)Cite this article

Abstract

Interleukin-22 (IL-22), a member of IL-10 family, plays some important roles in immune response through activation of the STAT 3 signal transduction pathway. Two types of IL-22-binding receptor have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 may be involved in inflammatory processes specifically regulated by soluble receptors. By screening a mouse genomic library for a human IL-22 binding protein homologue, we identified the mouse genomic clone of IL-22 binding protein. Its coding sequence was verified and isolated by RT–PCR. The gene encodes a protein of 230 amino acids that share 67.1% amino-acid sequence identity with human IL-22 binding protein. We designated this receptor ‘mouse IL-22 binding protein’ (mIL-22BP). mIL-22BP could be upregulated by LPS stimulation in mouse monocytes. mIL-22BP binds to mouse and human IL-22 and neutralizes STAT3 activation induced by both cytokines in human and rat hepatoma cell lines. Treating B cells with mouse IL-22 induces production of reactive oxygen species, which mIL-22BP blocks.

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Authors and Affiliations

  1. Institute of Basic Medical Sciences, National Cheng Kung University, Tainan, Taiwan

    Chi-Chen Wei

  2. Department of Medical Technology, Chung Hwa College of Medical Technology, Tainan, Taiwan

    Chi-Chen Wei

  3. Graduate Institute of Biochemistry, Medical College, National Cheng Kung University, Tainan, Taiwan

    T-W Ho, G-Y Chen & Ming-Shi Chang

  4. Chi-Mei Medical Center, Tainan, Taiwan

    W-G Liang & Ming-Shi Chang

Authors
  1. Chi-Chen Wei
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  2. T-W Ho
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  3. W-G Liang
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Corresponding author

Correspondence to Ming-Shi Chang.

Additional information

The nucleotide sequences of mouse binding protein have been deposited in the GenBank database with accession number AF493604.

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Wei, CC., Ho, TW., Liang, WG. et al. Cloning and characterization of mouse IL-22 binding protein. Genes Immun 4, 204–211 (2003). https://doi.org/10.1038/sj.gene.6363947

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