Abstract
A wide array of studies has demonstrated differences in genotype and allele frequencies of cytokine gene polymorphisms depending on ethnicity and race. In this study, the frequency of Taq-I polymorphism in 3′ untranslated region of IL-12B was investigated in two Bulgarian ethnic groups—Bulgarians and Turkish minority. No significant differences of genotype and allele frequencies were observed between these groups. Genotype distribution in the total group of Bulgarian citizens was: AA (61%), CA (32%) and CC (7%), and the allele frequency of 16974 A allele was 0.77. We also evaluated whether this polymorphism affects IL-12p40 production from human PBMC after stimulation. We demonstrated that association between genotype and IL-12p40 production by stimulated PBMC depends on the stimuli used. Our results indicated a significantly decreased IL-12 p40 secretion for the following order of genotypes: AA>CA>CC, after stimulation of PBMC with C3-binding glycoprotein (C3bgp) in contrast to lipopolysaccharide, phytohaemagglutinin and pokeweed mitogen.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 6 digital issues and online access to articles
$119.00 per year
only $19.83 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Hölscher C . The power of combinatorial immunology: dimeric cytokines in infectious diseases. Med Microbiol Immunol 2004; 193: 1–17.
Trinchieri G . Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nat Rev Immunol 2003; 3: 133–146.
Huang D, Cancilla MR, Morahan G . Complete primary structure, chromosomal localization and definition of polymorphisms of the gene encoding the human interleukin-12 p40 subunit. Genes Immun 2003; 1: 515–520.
Hall MA, McGlinn E, Coakley G et al. Genetic polymorphism of IL-12 p40 gene in immune-mediated disease. Genes Immun 2000; 1: 219–224.
Morahan G, Boutlis CS, Huang D et al. A promoter polymorphism in the gene encoding interleukin-12 p40 (IL-12B) is associated with mortality from cerebral malaria and with reduced nitric oxide production. Genes Immun 2002; 3: 414–418.
Golovleva I, Saha N, Beckman L . Ethnic differences in interferon-alpha allele frequencies. Hum Hered 1997; 47: 185–188.
Cox ED, Hoffmann SC, DiMercurio BS et al. Cytokine polymorphic analyses indicate ethnic differences in the allelic distribution of interleukin-2 and interleukin-6. Transplantation 2001; 72: 720–726.
De Capei MU, Dametto E, Fasano ME, Rendine S, Curtoni ES . Genotyping for cytokine polymorphisms: allele frequencies in the Italian population. Eur J Immunogenet 2003; 30: 5–10.
Seegers D, Zwiers A, Strober W, Pena AS, Bouma G . A TaqI polymorphism in the 3′ UTR of the IL-12 p40 gene correlates with increased IL-12 secretion. Genes Immun 2002; 3: 419–423.
Morahan G, Huang D, Ymer SI et al. Linkage disequilibrium of a type 1 diabetes susceptibility locus with a regulatory IL12B allele. Nat Genet 2001; 27: 218–221.
Davoodi-Semiromi A, Yang J, She JX . IL-12p40 is associated with type 1 diabetes in Caucasian-American families. Diabetes 2002; 51: 2334–2336.
Yilmaz V, Parmaan YG, Serdaroglu P, Deymeer F, Saruhan-Direskeneli G . IL-12 and IL-10 polymorphisms in Myastenia gravis. Neuroanatomy 2003; 2 (Suppl 1): 1 (abstr. 4).
Uyar FA, Dorak MT, Saruhan-Direskeneli G . Human leukocyte antigen-A, -B and -C alleles and human leukocyte antigen haplotypes in Turkey: relationship to other populations. Tissue Antigens 2004; 64: 180–187.
Ross OA, Curran MD, Meenagh A et al. Study of age-association with cytokine gene polymorphisms in an aged Irish population. Mech Ageing Dev 2003; 124: 199–206.
Latsi P, Pantelidis P, Vassilakis D, Sato H, Welsh KI, du Bois RM . Analysis of IL-12 p40 subunit gene and IFN-γ G5644A polymorphisms in idiopathic pulmonary fibrosis. Resp Res 2003; 4: 6.
Tsunemi Y, Saeki H, Nakamura K et al. Interleukin-12 p40 gene (IL12B) 3′-untranslated region polymorphism is associated with susceptibility to atopic dermatitis and psoriasis. J Dermatol Sci 2002; 30: 161–166.
Zhelev Z, Stanilova S, Carpenter B . Isolation, partial characterization and complement inhibiting activity of a new glycoprotein from Cuscuta europea. Biochem Biophys Res Commun 1994; 202: 186–194.
Stanilova S, Dobreva Z, Zhelev Zh . Changes in serum levels of cytokines in mice injected with an immunostimulator C3bgp isolated from Cuscuta europea. Int Immunopharmacol 2001; 1: 1597–1604.
Morahan G, Huang D, Wu M et al. Association of IL12B promoter polymorphism with severity of atopic and non-atopic asthma in children. Lancet 2002; 360: 455–459.
Muller-Berghaus J, Kern K, Pasechen A et al. Deficient IL-12p70 secretion by dendritic cells based on IL12B promoter genotype. Genes Immun 2004; 5: 431–434.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Stanilova, S., Miteva, L. Taq-I polymorphism in 3′UTR of the IL-12B and association with IL-12p40 production from human PBMC. Genes Immun 6, 364–366 (2005). https://doi.org/10.1038/sj.gene.6364213
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.gene.6364213
Keywords
This article is cited by
-
IL12B gene polymorphisms have sex-specific effects in relapsing–remitting multiple sclerosis
Acta Neurologica Belgica (2019)
-
Association of IL12B risk haplotype and lack of interaction with HLA-Cw6 among the psoriasis patients in India
Journal of Human Genetics (2017)
-
Association of Interleukin-12A rs568408 with Susceptibility to Asthma in Taiwan
Scientific Reports (2017)
-
Increased expression of IL12B mRNA transcribed from the risk haplotype for Crohn’s disease is a risk factor for disease relapse in Japanese patients
Journal of Gastroenterology (2017)
-
Inflammatory Cytokines and Alzheimer’s Disease: A Review from the Perspective of Genetic Polymorphisms
Neuroscience Bulletin (2016)