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  • Original Paper
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Bis, a Bcl-2-binding protein that synergizes with Bcl-2 in preventing cell death

Oncogene volume 18, pages 6183–6190 (1999)Cite this article

Abstract

Bcl-2 is the best characterized inhibitor of apoptosis, although the molecular basis of this action is not fully understood. Using a protein interaction cloning procedure, we identified a human gene designated as bis (mapped to chromosome 10q25) that encoded a novel Bcl-2-interacting protein. Bis protein showed no significant homology with Bcl-2 family proteins and had no prominent functional motif. Co-immunoprecipitation analysis confirmed that Bis interacted with Bcl-2 in vivo. DNA transfection experiments indicated that Bis itself exerted only weak anti-apoptotic activity, but was synergistic with Bcl-2 in preventing Bax-induced and Fas-mediated apoptosis. These results suggest that Bis is a novel modulator of cellular anti-apoptotic activity that functions through its interaction with Bcl-2.

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Acknowledgements

We thank Dr D Mason for the generous gift of hBcl-2 monoclonal antibody. This work was supported in part by grants for Scientific Research on Priority Areas, for Center of Excellence Research, and for JSPS Fellows from the Japanese Ministry of Education, Science, Sports and Culture.

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Authors and Affiliations

  1. Department of Medical Genetics, Biomedical Research Center, Osaka University Graduate School of Medicine, Osaka, Japan

    Jeong-Hwa Lee, Takeshi Takahashi, Noriko Yasuhara, Shinji Kamada & Yoshihide Tsujimoto

  2. CREST of Japan Science and Technology Corp. (JST), 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Jeong-Hwa Lee & Yoshihide Tsujimoto

  3. Department of Molecular Cytogenetics, Division of Genetics, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8519, Japan

    Joji Inazawa

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Lee, JH., Takahashi, T., Yasuhara, N. et al. Bis, a Bcl-2-binding protein that synergizes with Bcl-2 in preventing cell death. Oncogene 18, 6183–6190 (1999). https://doi.org/10.1038/sj.onc.1203043

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