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Cleavage of Bcl-2 in oxidant- and cisplatin-induced apoptosis of human melanoma cells

Oncogene volume 20, pages 4591–4595 (2001)Cite this article

Abstract

Although the anti-apoptotic effect of Bcl-2 is well established, the role of Bcl-2 in tumour response to therapy and drug resistance is still unclear. The post-translational modifications of Bcl-2 are likely involved in the control of the apoptotic pathway. In the present study we have investigated the role of Bcl-2 in cellular response to oxidative stress (hydrogen peroxide) and cisplatin using a clone of human metastatic melanoma, which, in spite of Bcl-2 (over)expression, exhibited a moderate chemosensitivity. With both treatments melanoma cells died through an apoptotic process, associated with detachment of cells from the monolayer. In the floating apoptotic cells generated by either hydrogen peroxide or cisplatin, along with morphological and biochemical features of apoptosis, we detected a significant Bcl-2 cleavage, yielding the Bax-like fragment of 23 kDa. Preincubation of cells with the caspase-3/-7 inhibitor DEVD-CHO completely suppressed Bcl-2 cleavage, thus confirming that such a specific proteolysis requires activation of caspase-3/-7. The oxidant- and cisplatin-induced processing of Bcl-2 documented in the present study may represent a regulatory mechanism to circumvent the survival function of Bcl-2 upon apoptosis triggering and to enhance apoptotic response. Since the Bcl-2 cleavage should be regarded as a pro-apoptotic event, Bcl-2 expression is expected to increase susceptibility to apoptosis. Thus, such a pathway could be exploited to improve the efficacy of cytotoxic therapy of melanomas expressing Bcl-2.

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Acknowledgements

The authors are indebted to Dr G Parmiani for making Me665/2 cells available for the present study. We are also grateful to Prof A Pompella and Dr P Perego for valuable discussions. This work was supported by the Association for International Cancer Research (AICR, UK), by Italian Ministry of University and Scientific Research (MURST-Cofinanziamento 1998), and by grants of University of Siena (PAR).

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Authors and Affiliations

  1. Department of Pathophysiology and Experimental Medicine, University of Siena, via A. Moro–53100 Siena, Italy

    Barbara Del Bello, Marta A Valentini, Mario Comporti & Emilia Maellaro

  2. Istituto Nazionale Tumori, via Venezian 1–20133, Milan, Italy

    Franco Zunino

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  1. Barbara Del Bello
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  2. Marta A Valentini
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  3. Franco Zunino
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  4. Mario Comporti
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  5. Emilia Maellaro
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Correspondence to Emilia Maellaro.

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Del Bello, B., Valentini, M., Zunino, F. et al. Cleavage of Bcl-2 in oxidant- and cisplatin-induced apoptosis of human melanoma cells. Oncogene 20, 4591–4595 (2001). https://doi.org/10.1038/sj.onc.1204618

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