The purification, structural characterization and antidiabetic activity of a polysaccharide from Anoectochilus roxburghii

Yuntao Liu; Tingting Tang; Songqi Duan; Cheng Li; Qinlu Lin; Hejun Wu; Aiping Liu; Bin Hu; Dingtao Wu; Suqing Li; Li Shen; Wenjuan Wu

doi:10.1039/C9FO00860H

The purification, structural characterization and antidiabetic activity of a polysaccharide from Anoectochilus roxburghii

Yuntao Liu,

†*^abc Tingting Tang,†^a Songqi Duan,†^a Cheng Li,^a Qinlu Lin,^c Hejun Wu,^a Aiping Liu,^a Bin Hu,*^a Dingtao Wu,

^a Suqing Li,^a Li Shen^a and Wenjuan Wu^d

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* Corresponding authors

^a College of Food Science, Sichuan Agricultural University, Yaan 625014, China
E-mail: lyt_taotao@163.com, nutritionlab@sicau.edu.cn, 714351706@qq.com
Fax: +86 835 2882187
Tel: +86 835 2882187

^b Tianjin Key Laboratory of Food Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China

^c National Engineering Laboratory for Rice and By-product Deep Processing, College of Food Science and Engineering, Central South University of Forestry & Technology, Changsha 410004, China

^d College of Science, Sichuan Agricultural University, Yaan 625014, China

Abstract

Anoectochilus roxburghii, a traditional Chinese medicinal herb, has been widely used for treating numerous chronic diseases. In this study, a polysaccharide from A. roxburghii (ARPs-p) was purified by anion exchange and size exclusion chromatography. The structural characteristics of ARPs-p were systematically investigated for the first time via numerous chromatographic techniques, periodic acid oxidation, Smith degradation, methylation analysis, FTIR spectroscopy and 1D/2D NMR spectroscopy. The results showed that ARPs-p is a heteropolysaccharide with a molecular weight of 97 kDa; it consists of 97.75% glucose, 1.2% galactose and trace amounts of galacturonic acid, and its backbone structure is composed of →3)-β-D-Glcp-(1→ with some branching points at O-6 linked to non-reducing end units or 6-O-linked Glcp units. Furthermore, streptozotocin (STZ)-induced diabetic mouse experiments suggested that ARPs-p has excellent antihyperglycemic, antioxidant and antihyperlipidemic activities, in which 1,3-β-D-glucan, the main component of ARPs-p, plays a vital role.

Food & Function

The purification, structural characterization and antidiabetic activity of a polysaccharide from Anoectochilus roxburghii

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The purification, structural characterization and antidiabetic activity of a polysaccharide from Anoectochilus roxburghii

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