Abstract
Selected bacterial strains that are responsible for period-ontal diseases are efficiently inactivated by visible light irradiation in the presence of porphycene–polylysine conjugates. Repeated photosensitization of surviving cells does not induce the selection of resistant bacterial strains and does not modify their sensitivity to antibiotic treatment.
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M. Wainwright, Photodynamic antimicrobial chemotherapy, J. Antimicrob. Chemother., 1998, 42, 13–28.
G. Jori, PDT for the treatment of microbial infections, Photodyn. News, 1999, 2, 2–3.
G. Bertoloni, F. Rossi, G. Jori, G. Valduga, H. Ali and J. E. van Lier, Photosensitizing activity of water- and lipid-soluble phthalo-cyanines on eukaryotic and prokaryotic microbial cells, Microbios, 1992, 71, 33–46.
M. Merchat, G. Bertoloni, P. Giacomoni, A. Villanueva and G. Jori, Meso-substituted cationic porphyrins as efficient photosensitizers of Gram-positive and Gram-negative bacteria, J. Photochem. Photobiol., B, 1996, 32, 153–157.
A. Minnock, D. I. Vernon, J. Griffiths, J. H. Parish and S. B. Brown, Photoinactivation of bacteria. Use of a cationic water-soluble zinc phthalocyanine to photoinactivate both Gram-negative and Grampositive bacteria, J. Photochem. Photobiol., B, 1996, 32, 159–164.
Z. Malik, H. Ladan and Y. Nitzan, Photodynamic inactivation of Gram-negative bacteria: problems and possible solutions, J. Photochem. Photobiol., B, 1992, 14, 262–266.
M. Wilson, Photolysis of oral bacteria and its potential use in the treatment of caries and periodontal diseases, J. Appl. Bacteriol., 1993, 78, 299–308.
R. W. Redmond, G. Valduga, S. Nonell, S. E. Braslavsky, K. Schaffner, E. Vogel, K. Pramod and M. Kochner, The photophysical properties of porphycenes incorporated in small unilamellar lipid vesicles, J. Photochem. Photobiol., B, 1989, 3, 193–198.
L. Polo, A. Segalla, G. Bertoloni, G. Jori, K. Schaffner and E. Reddi, Polylysine-porphycene conjugates as efficient photosensitizers for the inactivation of microbial pathogens, J. Photochem. Photobiol., B, 2000, 59, 152–158.
J. F. Acar and F. W. Goldstein, Disk susceptibility test, in Antibiotics in Laboratory Medicine, ed. V. Lorian, New York, 1966, pp. 1–51.
T. J. Dougherty, C. J. Gomer, B. W. Henderson, G. Jori, D. Kessel, M. Korbelik, J. Moan and A. Peng, Photodynamic therapy, J. Natl. Cancer Inst., 1998, 90, 889–905.
N. S. Soukos, M. R. Hamblin and T. Hasan, The effect of charge on cellular uptake and phototoxicity of polylysine-chlorin e6 conjugates, Photochem. Photobiol., 1997, 65, 723–729.
M. A. Listgarten, The pathogenesis of periodontitis, J. Clin. Perio-dontol., 1986, 13, 418–430.
M. Wilson, Susceptibility of oral bacteria biofilms to antimicrobial agents, J. Med. Microbiol., 1996, 44, 79–87.
S. Sarkar and M. Wilson, Lethal photosensitization of bacteria in supragingival plaque from patients with chronic periodontitis, J. Periodontal Res., 1993, 28, 204–210.
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Lauro, F.M., Pretto, P., Covolo, L. et al. Photoinactivation of bacterial strains involved in periodontal diseases sensitized by porphycene–polylysine conjugates. Photochem Photobiol Sci 1, 468–470 (2002). https://doi.org/10.1039/b200977c
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DOI: https://doi.org/10.1039/b200977c