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Photoinactivation of bacterial strains involved in periodontal diseases sensitized by porphycene–polylysine conjugates

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Abstract

Selected bacterial strains that are responsible for period-ontal diseases are efficiently inactivated by visible light irradiation in the presence of porphycene–polylysine conjugates. Repeated photosensitization of surviving cells does not induce the selection of resistant bacterial strains and does not modify their sensitivity to antibiotic treatment.

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References

  1. M. Wainwright, Photodynamic antimicrobial chemotherapy, J. Antimicrob. Chemother., 1998, 42, 13–28.

    Article  CAS  Google Scholar 

  2. G. Jori, PDT for the treatment of microbial infections, Photodyn. News, 1999, 2, 2–3.

    Google Scholar 

  3. G. Bertoloni, F. Rossi, G. Jori, G. Valduga, H. Ali and J. E. van Lier, Photosensitizing activity of water- and lipid-soluble phthalo-cyanines on eukaryotic and prokaryotic microbial cells, Microbios, 1992, 71, 33–46.

    CAS  PubMed  Google Scholar 

  4. M. Merchat, G. Bertoloni, P. Giacomoni, A. Villanueva and G. Jori, Meso-substituted cationic porphyrins as efficient photosensitizers of Gram-positive and Gram-negative bacteria, J. Photochem. Photobiol., B, 1996, 32, 153–157.

    Article  CAS  Google Scholar 

  5. A. Minnock, D. I. Vernon, J. Griffiths, J. H. Parish and S. B. Brown, Photoinactivation of bacteria. Use of a cationic water-soluble zinc phthalocyanine to photoinactivate both Gram-negative and Grampositive bacteria, J. Photochem. Photobiol., B, 1996, 32, 159–164.

    Article  CAS  Google Scholar 

  6. Z. Malik, H. Ladan and Y. Nitzan, Photodynamic inactivation of Gram-negative bacteria: problems and possible solutions, J. Photochem. Photobiol., B, 1992, 14, 262–266.

    Article  CAS  Google Scholar 

  7. M. Wilson, Photolysis of oral bacteria and its potential use in the treatment of caries and periodontal diseases, J. Appl. Bacteriol., 1993, 78, 299–308.

    Article  Google Scholar 

  8. R. W. Redmond, G. Valduga, S. Nonell, S. E. Braslavsky, K. Schaffner, E. Vogel, K. Pramod and M. Kochner, The photophysical properties of porphycenes incorporated in small unilamellar lipid vesicles, J. Photochem. Photobiol., B, 1989, 3, 193–198.

    Article  CAS  Google Scholar 

  9. L. Polo, A. Segalla, G. Bertoloni, G. Jori, K. Schaffner and E. Reddi, Polylysine-porphycene conjugates as efficient photosensitizers for the inactivation of microbial pathogens, J. Photochem. Photobiol., B, 2000, 59, 152–158.

    Article  CAS  Google Scholar 

  10. J. F. Acar and F. W. Goldstein, Disk susceptibility test, in Antibiotics in Laboratory Medicine, ed. V. Lorian, New York, 1966, pp. 1–51.

    Google Scholar 

  11. T. J. Dougherty, C. J. Gomer, B. W. Henderson, G. Jori, D. Kessel, M. Korbelik, J. Moan and A. Peng, Photodynamic therapy, J. Natl. Cancer Inst., 1998, 90, 889–905.

    Article  CAS  Google Scholar 

  12. N. S. Soukos, M. R. Hamblin and T. Hasan, The effect of charge on cellular uptake and phototoxicity of polylysine-chlorin e6 conjugates, Photochem. Photobiol., 1997, 65, 723–729.

    Article  CAS  Google Scholar 

  13. M. A. Listgarten, The pathogenesis of periodontitis, J. Clin. Perio-dontol., 1986, 13, 418–430.

    Article  CAS  Google Scholar 

  14. M. Wilson, Susceptibility of oral bacteria biofilms to antimicrobial agents, J. Med. Microbiol., 1996, 44, 79–87.

    Article  CAS  Google Scholar 

  15. S. Sarkar and M. Wilson, Lethal photosensitization of bacteria in supragingival plaque from patients with chronic periodontitis, J. Periodontal Res., 1993, 28, 204–210.

    Article  CAS  Google Scholar 

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Correspondence to Giulio Jori.

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Lauro, F.M., Pretto, P., Covolo, L. et al. Photoinactivation of bacterial strains involved in periodontal diseases sensitized by porphycene–polylysine conjugates. Photochem Photobiol Sci 1, 468–470 (2002). https://doi.org/10.1039/b200977c

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  • DOI: https://doi.org/10.1039/b200977c

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