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Photodynamic effect in medulloblastoma: downregulation of matrix metalloproteinases and human telomerase reverse transcriptase expressions

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Abstract

Tumor invasion and immortality are the most unfavorable drawbacks after cancer treatment. In this study, we focus on determining the photodynamic modulation of the proteolytic enzymes, matrix metalloproteinases (MMP); and a core catalytic subunit of telomerase, the human telomerase reverse transcriptase (hTERT) in medulloblastoma (MED) cell line (TE-671). Hexvix (ALA-H) mediated photodynamic therapy (PDT) demonstrated greater efficacy than 5-aminolevulinic acid (5-ALA) in terms of drug uptake and anti-proliferative effect. Both MMP-2 and hTERT expression are down-regulated quantitatively using ELISA and reverse-transcriptase-PCR (RT-PCR) respectively at post-treatment for this cell line. The MMP-9 expression remains unchanged after treatment. Further, there is a statistically significant inhibition of cell migration at 24 h post-ALA-H-PDT at LD50 (0.01 mM, 2 J cm-2; p < 0.001) in MED TE-671 cells. Evidently, MMP-2 and hTERT mRNA expressions can be the targets for the photodynamic intervention on tumor cell migration and immortality. Hence,PDT may be an alternate cancer regime for medulloblastoma.

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Authors and Affiliations

  1. Medical Laboratory Science, Department of Health Technology & Informatics, The Hong Kong Polytechnic University, China

    Ellie Shihng Meir Chu & Christine Miu Ngan Yow

  2. Faculty of Health& Social Sciences, The Hong Kong Polytechnic University, China

    Thomas Kwok Shing Wong

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  1. Ellie Shihng Meir Chu
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Correspondence to Christine Miu Ngan Yow.

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Chu, E.S.M., Wong, T.K.S. & Yow, C.M.N. Photodynamic effect in medulloblastoma: downregulation of matrix metalloproteinases and human telomerase reverse transcriptase expressions. Photochem Photobiol Sci 7, 76–83 (2008). https://doi.org/10.1039/b703417b

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