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The effect of Lactobacillus rhamnosus HN001 on mineral absorption and bone health in growing male and ovariectomised female rats

HN001

Effet de Lactobacillus rhamnosus HN001 sur l’absorption minérale et la santé osseuse chez le rat mâle en croissance et la ratte ovariectomisée

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Dairy Science & Technology

Abstract

Prebiotics affect mineral absorption, in part by increasing solubility due to short chain fatty acid generation. Prebiotics stimulate the growth of probiotics in the gut and probiotics may affect mineral absorption by similar mechanisms. The objectives of this study were to measure (1) the effect of Lactobacillus rhamnosus strain HN001 (HN001) on mineral absorption in growing male rats and (2) whether HN001 can reduce bone loss and affect bone properties in female ovariectomised (OVX) rats. Study 1: Twenty-two 3-week-old Sprague-Dawley rats were weaned onto a milk powder-based diet supplying 20% protein and 0.5% calcium. After a week, the rats were randomised into two groups: one continuing on the base diet and the other receiving 109 cfu HN001 per day in the diet. After 3 weeks, the animals were housed in metabolic cages for 8 days. Food intake was measured, and urine and faeces were collected for 3 days. Calcium and magnesium balances were calculated. Study 2: Forty 5.5-month-old female Sprague-Dawley rats were ovariectomised or sham-operated and randomised into three groups. The sham (n = 10) and OVX control groups (n = 15) remained on a caseinate-based control diet, while the experimental group (n = 15) received 109 cfu HN001 per day in the diet. After 12 weeks, bone density was measured by dual energy X-ray absorptiometry and bone and blood samples were collected. HN001 improved calcium and magnesium retention in the growing male rats, but due to differences in food intake, data was inconclusive. However, HN001-fed OVX rats had a reduced rate of bone loss and a higher final bone density in the spine and femur at week 12 compared to the OVX control group. HN001 may improve mineral bioavailability and have a positive effect on bone mineral density and mineral content. The underlying mechanisms need to be researched.

Abstract

(1) Lb HN001 (Lactobacillus rhamnosus) ; (2) Lb HN001 (OVX) : (1) 22 3 Sprague-Dawley 20% 0.5% 109 cfu Lb HN001 3 8 d 3 d (2) 40 5.5 Sprague-Dawley 3 (n = 10) OVX (n = 15) (n = 15) 109 cfu Lb HN001 12 X Lb HN001 OVX 12 Lb HN001-OVX Lb HN001

Résumé

Les prébiotiques ont un effet sur l’absorption minérale, en partie en augmentant la solubilité due à la génération d’acides gras à chaîne courte. Les prébiotiques stimulent la croissance des probiotiques dans l’intestin et les probiotiques peuvent affecter l’absorption minérale par des mécanismes similaires. Les objectifs de l’étude ont été de mesurer : (1) l’effet de Lactobacillus rhamnosus HN001 (HN001) sur l’absorption minérale chez le rat mâle en croissance, (2) si HN001 peut réduire la perte osseuse et affecter les propriétés des os chez la ratte ovariectomisée (OVX). Étude 1 : 22 rats Sprague-Dawley de 3 semaines ont été sevrés puis soumis à un régime à base de poudre de lait apportant 20 % de protéines et 0,5 % de calcium. Après une semaine, les rats ont été répartis au hasard en deux groupes : l’un continuant le régime de base, l’autre recevant en plus 109 ufc HN001 par jour. Après 3 semaines, les animaux ont été installés dans des cages métaboliques pour 8 jours. La prise alimentaire a été mesurée, et l’urine et les fèces récoltés pendant 3 jours. Les équilibres en calcium et magnésium ont été calculés. Étude 2 : 40 rattes Sprague-Dawley de 5 mois et demi ont été ovariectomisées ou opérées de façon fictive puis réparties au hasard en 3 groupes. Les groupes « fictif » (n = 10) ou OVX de contrôle (n = 15) étaient maintenus au régime contrôle à base de caséinate tandis que le groupe expérimental (n = 15) recevait le même régime additionné de 109 ufc HN001 par jour. Après 12 semaines, la densité osseuse a été mesurée par absorptiométrie à rayons X biphotonique et des échantillons d’os et de sang ont été collectés. HN001 améliorait la rétention de calcium et magnésium chez les rats mâles en croissance, mais, en raison de différences dans la prise alimentaire, les données étaient peu concluantes. Cependant, les rattes OVX nourries avec HN001 montraient moins de perte osseuse et une densité osseuse finale plus élevée dans la colonne vertébrale et le fémur à 12 semaines que les rattes du groupe contrôle. HN001 pourrait améliorer la biodisponibilité minérale et avoir un effet positif sur la densité minérale osseuse et la teneur en minéraux. Les mécanismes qui sous-tendent cette activité restent à déterminer.

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Authors and Affiliations

  1. Institute of Food, Nutrition and Human Health, Private Bag 11222, Palmerston North, New Zealand

    Marlena Cathorina Kruger, Alison Fear, Wei-Hang Chua & Gabrielle Grey Plimmer

  2. Fonterra Research Centre, Private Bag 11029, Palmerston North, New Zealand

    Linda May Schollum

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  1. Marlena Cathorina Kruger
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  2. Alison Fear
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  3. Wei-Hang Chua
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Correspondence to Marlena Cathorina Kruger.

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Kruger, M.C., Fear, A., Chua, WH. et al. The effect of Lactobacillus rhamnosus HN001 on mineral absorption and bone health in growing male and ovariectomised female rats. Dairy Sci. Technol. 89, 219–231 (2009). https://doi.org/10.1051/dst/2009012

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  • DOI: https://doi.org/10.1051/dst/2009012

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