1887

Abstract

An aerobic, Gram-negative, non-motile, yellow-to-orange pigmented and rod-shaped bacterium, designated strain SM1, was isolated from seawater in Thailand and subjected to a polyphasic taxonomic study. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the novel isolate shared 93–95 % sequence similarity with species of the genus . The DNA–DNA relatedness values of strain SM1 with JCM 7460 and JCM 7458 were below 70 %. The DNA G+C content of strain SM1 was 33.6 mol%, the major menaquinone was MK-6 and the major fatty acids were C iso, C 9 iso and C iso 3-OH. Based on its phenotypic and chemotaxonomic characteristics, strain SM1 was classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SM1 (=IAM 15337=KCTC 12661).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64336-0
2006-08-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/8/1917.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64336-0&mimeType=html&fmt=ahah

References

  1. Atlas R. M. 2004 Handbook of Microbiological Media , 3rd edn. Boca Raton, FL: CRC Press;
    [Google Scholar]
  2. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  5. Holmes B., Snell J. J. S., Lapage S. P. 1977; Revised description, from clinical isolates, of Flavobacterium odoratum Stutzer and Kwaschnina 1929, and designation of the neotype strain. Int J Syst Bacteriol 27:330–336 [CrossRef]
    [Google Scholar]
  6. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  7. Maneerat S., Nitoda T., Kanzaki H., Kawai F. 2005; Bile acids are new products of a marine bacterium, Myroides sp. strain SM1. Appl Microbiol Biotechnol 67:679–683 [CrossRef]
    [Google Scholar]
  8. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  9. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  10. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  11. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  12. Swofford D. L. 1998 paup* - Phylogenetic analysis using parsimony (*and other methods), version 4 Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  13. Vancanneyt M., Segers P., Torck U., Hoste B., Bernardet J.-F., Vandamme P., Kersters K. 1996; Reclassification of Flavobacterium odoratum (Stutzer 1929) strains to a new genus, Myroides , as Myroides odoratus comb.nov. and Myroides odoratimimus sp. nov. Int J Syst Bacteriol 46:926–932 [CrossRef]
    [Google Scholar]
  14. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  15. Xie C. H., Yokota A. 2003; Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49:345–349 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64336-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64336-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error