1887

Abstract

Genetically highly divergent picornavirus (Newt/2013/HUN, KP770140) was detected using viral metagenomics in faecal samples of free-living smooth newts (). Newt picornavirus was identified by reverse transcription-polymerase chain reaction (RT-PCR) in six (25 %) of the 24 samples originating from individuals caught in two out of the six investigated natural ponds in Hungary. The first picornavirus in amphibians expands the host range of members of the , and opens a new research field in picornavirus evolution in lower vertebrates. Newt picornavirus represents a novel species in a novel genus within the family , provisionally named genus (amphibian picornavirus).

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2015-09-01
2024-04-26
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References

  1. APHA 1985 Standard Methods for the Examination of Wastewater, 16th edn. Washington, DC: American Public Health Association;
    [Google Scholar]
  2. Arntzen J.W., Kuzmin S., Beebee T., Papenfuss T., Sparreboom M., Ugurtas I.H., Anderson S., Anthony B., Andreone F., other authors. 2009 Lissotriton vulgaris The IUCN Red List of Threatened Species. Version 2014.3 www.iucnredlist.org Downloaded on 3 December 2014
    [Google Scholar]
  3. Barbknecht M., Sepsenwol S., Leis E., Tuttle-Lau M., Gaikowski M., Knowles N.J., Lasee B., Hoffman M.A. 2014; Characterization of a new picornavirus isolated from the freshwater fish Lepomis macrochirus . J Gen Virol 95:601–613 [View Article][PubMed]
    [Google Scholar]
  4. Boros Á., Pankovics P., Simmonds P., Reuter G. 2011; Novel positive-sense, single-stranded RNA (+ssRNA) virus with di-cistronic genome from intestinal content of freshwater carp (Cyprinus carpio). PLoS One 6:e29145 [View Article][PubMed]
    [Google Scholar]
  5. Boros Á., Pankovics P., Reuter G. 2014; Avian picornaviruses: molecular evolution, genome diversity and unusual genome features of a rapidly expanding group of viruses in birds. Infect Genet Evol 28:151–166 [View Article][PubMed]
    [Google Scholar]
  6. Chen H.H., Kong W.P., Roos R.P. 1995; The leader peptide of Theiler's murine encephalomyelitis virus is a zinc-binding protein. J Virol 69:8076–8078[PubMed]
    [Google Scholar]
  7. Collins J.P., Brunner J.L., Jancovich J.K., Schock D.M. 2004; A model host-pathogen system for studying infectious disease dynamics in amphibians: tiger salamanders (Ambystoma tigrinum) and Ambystoma tigrinum virus. Herpetol J 14:195–200
    [Google Scholar]
  8. Fichtner D., Philipps A., Groth M., Schmidt-Posthaus H., Granzow H., Dauber M., Platzer M., Bergmann S.M., Schrudde D., other authors. 2013; Characterization of a novel picornavirus isolate from a diseased European eel (Anguilla anguilla). J Virol 87:10895–10899[PubMed] [CrossRef]
    [Google Scholar]
  9. Frost D. 2014 American Museum of Natural History: Amphibian species of the World 6.0.Online reference retrieved: 28 November 2014. http://research.amnh.org/vz/herpetology/amphibia/
  10. Gorbalenya A.E., Koonin E.V., Lai M.M. 1991; Putative papain-related thiol proteases of positive-strand RNA viruses. Identification of rubi- and aphthovirus proteases and delineation of a novel conserved domain associated with proteases of rubi-, alpha- and coronaviruses. FEBS Lett 288:201–205 [View Article][PubMed]
    [Google Scholar]
  11. Green D.E., Converse K.A., Schrader A.K. 2002; Epizootiology of sixty-four amphibian morbidity and mortality events in the USA, 1996-2001. Ann N Y Acad Sci 969:323–339 [View Article][PubMed]
    [Google Scholar]
  12. Heuser W., Kaleta E., Giesow K., Keil G.M., Knowles N.J. 2010; Genome sequence of virus “X”, a picornavirus isolated from a spur-thighed tortoise (Testudo graeca). In EUROPIC 2010: XVI Meeting of the European Study Group on the Molecular Biology of Picornaviruses pp. 147 St Andrews, Scotland, UK, 11-16 September 2010. Abstract H15
    [Google Scholar]
  13. [Google Scholar]
  14. Kapoor A., Victoria J., Simmonds P., Wang C., Shafer R.W., Nims R., Nielsen O., Delwart E. 2008b; A highly divergent picornavirus in a marine mammal. J Virol 82:311–320 [View Article][PubMed]
    [Google Scholar]
  15. Knowles N.J., Hovi T., Hyypiä T., King A.M.Q., Lindberg A.M., Pallansch M.A., Palmenberg A.C., Simmonds P., Skern T., other authors. 2012; Picornaviridae. In Virus Taxonomy: Classification and Nomenclature of Viruses: Ninth Report of the International Committee on Taxonomy of Viruses pp. 855–880 Edited by King A. M. Q., Adams M. J., Carstens E. B., Lefkowitz E. J. San Diego: Elsevier;
    [Google Scholar]
  16. Lange J., Groth M., Fichtner D., Granzow H., Keller B., Walther M., Platzer M., Sauerbrei A., Zell R. 2014; Virus isolate from carp: genetic characterization reveals a novel picornavirus with two aphthovirus 2A-like sequences. J Gen Virol 95:80–90 [View Article][PubMed]
    [Google Scholar]
  17. Lauber C., Gorbalenya A.E. 2012; Toward genetics-based virus taxonomy: comparative analysis of a genetics-based classification and the taxonomy of picornaviruses. J Virol 86:3905–3915[PubMed] [CrossRef]
    [Google Scholar]
  18. Ng T.F.F., Wellehan J.F., Coleman J.K., Kondov N.O., Deng X., Waltzek T.B., Reuter G., Knowles N.J., Delwart E. 2015; A tortoise-infecting picornavirus expands the host range of the family Picornaviridae . Arch Virol 160:1319–1323 [View Article][PubMed]
    [Google Scholar]
  19. Nöllert A., Nöllert C. 1992 Die Amphibien Europas Stuttgart: Bestimmung-Gefährdung-Schutz. Franckh-Kosmos Verlag;
    [Google Scholar]
  20. Phan T.G., Vo N.P., Boros Á., Pankovics P., Reuter G., Li O.T., Wang C., Deng X., Poon L.L., Delwart E. 2013; The viruses of wild pigeon droppings. PLoS One 8:e72787 [View Article][PubMed]
    [Google Scholar]
  21. Phelps N.B.D., Mor S.K., Armien A.G., Batts W., Goodwin A.E., Hopper L., McCann R., Ng T.F.F., Puzach C., other authors. 2014; Isolation and molecular characterization of a novel picornavirus from baitfish in the USA. PLoS One 9:e87593 [View Article][PubMed]
    [Google Scholar]
  22. Racaniello V. 2007; Picornaviridae: the viruses and their replication. In Fields Virology, 5th edn. pp. 795–838 Edited by Knipe D. M., Howley P. M. Philadelphia: Lippincott Williams & Wilkins;
    [Google Scholar]
  23. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: Molecular Evolutionary Genetics Analysis version 6.0.. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  24. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  25. Victoria J.G., Kapoor A., Li L., Blinkova O., Slikas B., Wang C., Naeem A., Zaidi S., Delwart E. 2009; Metagenomic analyses of viruses in stool samples from children with acute flaccid paralysis. J Virol 83:4642–4651 [View Article][PubMed]
    [Google Scholar]
  26. Woo, P. C., Lau, S. K., Choi, G. K., Huang, Y., Teng, J. L., Tsoi, H. W., Tse, H., Yeung, M. L., Chan, K. H., other authors Woo P.C., Lau S.K., Choi G.K., Huang Y., Teng J.L., Tsoi H.W., Tse H., Yeung M.L., Chan K.H., other authors. 2012; Natural occurrence and characterization of two internal ribosome entry site elements in a novel virus, canine picodicistrovirus, in the picornavirus-like superfamily. J Virol 86:2797–2808[PubMed] [CrossRef]
    [Google Scholar]
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