FT-Raman spectroscopy study of human breast tissue

Renata Andrade Bitar Carter; Airton Abrahao Martin; Mario M. Netto M.D.; Fernando A. Soares M.D.

doi:10.1117/12.527797

1 July 2004 FT-Raman spectroscopy study of human breast tissue

Renata Andrade Bitar Carter, Airton Abrahao Martin, Mario M. Netto M.D., Fernando A. Soares M.D.

Proceedings Volume 5321, Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies; (2004) https://doi.org/10.1117/12.527797
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Optical spectroscopy has been extensively studied as a potential in vivo diagnostic tool to provide information about the chemical and morphologic structure of tissue. Raman Spectroscpy is an inelastic scattering process that can provide a wealth of spectral features that can be related to the specific molecular structure of the sample. This article reports results of an in vitro study of the FT-Raman human breast tissue spectra. An Nd:YAG laser at 1064nm was used as the excitation source in the FT-Raman Spectrometer. The neoplastic human breast samples, both Fibroadenoma and ICD, were obtained during therapeutical routine medical procedures required by the primary disease, and the non-diseased human tissue was obtained in plastic surgery. No sample preparation was needed for the FT-Raman spectra collection. The FT-Raman spectra were recorded from normal, benign (Fibroadenomas) and malignant (IDC-Intraductal Carcinoma) samples, adding up 51 different areas. The main spectral differences of a typical FT-Raman spectra of a Normal (Non-diseased), Fibroadenoma, and Infiltrating Ductal Carcinoma (IDC) breast tissue at the interval of 600 to 1800cm^-1, which may differentiate diagnostically the sample, were found in the bands of 1230 to 1295cm^-1, 1440 to 1460 cm^-1 and 1650 to 1680 cm^-1, assigned to the vibrational bands of the carbohydrate-amide III, proteins and lipids, and carbohydrate-amide I, respectively.

Citation Download Citation

Renata Andrade Bitar Carter, Airton Abrahao Martin, Mario M. Netto M.D., and Fernando A. Soares M.D. "FT-Raman spectroscopy study of human breast tissue", Proc. SPIE 5321, Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies, (1 July 2004); https://doi.org/10.1117/12.527797

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