Skip to main content
SpringerLink
Log in

Purification and primary structure of novel lipid transfer proteins from germinated lentil (Lens culinaris) seeds

  • Published:
Biochemistry (Moscow) Aims and scope Submit manuscript

Abstract

A subfamily of eight novel lipid transfer proteins designated as Lc-LTP1-8 was found in the lentil Lens culinaris. Lc-LTP2, Lc-LTP4, Lc-LTP7, and Lc-LTP8 were purified from germinated lentil seeds, and their molecular masses (9268.7, 9282.7, 9121.5, 9135.5 daltons) and complete amino acid sequences were determined. The purified proteins consist of 92–93 amino acid residues, have four disulfide bonds, and inhibit growth of Agrobacterium tumefaciens. Total RNA was isolated from germinated lentil seeds, RT-PCR and cloning were performed, and the cDNAs of six LTPs were sequenced. Precursor 116–118-residue proteins with 24–25-residue signal peptides were found, and two of them are purified proteins Lc-LTP2 and Lc-LTP4.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

FPLC:

fast protein liquid chromatography

LTP:

lipid transfer protein

MALDI-TOF-MS:

matrix-assisted laser desorption ionization time-of-flight mass spectrometry

PR-proteins:

pathogenesis-related proteins

RACE:

rapid amplification of cDNA ends

RT-PCR:

reverse trnascription polymerase chain reaction

References

  1. Selitrennikoff, C. P. (2001) Appl. Environ. Microbiol., 67, 2883–2894.

    Article  PubMed  CAS  Google Scholar 

  2. Hoffmann-Sommergruber, K. (2002) Biochem. Soc. Trans., 30, 930–935.

    Article  PubMed  CAS  Google Scholar 

  3. Castro, M. S., and Fontes, W. (2005) Prot. Pept. Lett., 12, 13–18.

    CAS  Google Scholar 

  4. Molina, A., Segura, A., and Garcia-Olmedo, F. (1993) FEBS Lett., 316, 119–122.

    Article  PubMed  CAS  Google Scholar 

  5. Douliez, J., Michon, T., Elmorjani, K., and Marion, D. (2000) J. Cereal Sci., 32, 1–20.

    Article  CAS  Google Scholar 

  6. Kader, J. (1996) Annu. Rev. Plant Physiol. Plant Mol. Biol., 47, 627–654.

    Article  PubMed  CAS  Google Scholar 

  7. Garcia-Olmedo, F., Molina, A., Alamillo, J. M., and Rodriguez-Palenzuella, P. (1999) Biopolymers, 47, 479–491.

    Article  Google Scholar 

  8. Samuel, D., Liu, Y., Cheng, C., and Lyu, P. (2002) J. Biol. Chem., 277, 35267–35273.

    Article  PubMed  CAS  Google Scholar 

  9. Kader, J. (1997) Trends Plant Sci., 2, 66–70.

    Article  Google Scholar 

  10. Hughes, M. A., Dunn, M. A., Pearce, R. S., White, A. J., and Zhang, L. (1992) Plant Cell Environ., 15, 861–865.

    Article  CAS  Google Scholar 

  11. Hincha, D. K. (2002) Phil. Trans. R. Soc. Lond., 357, 909–916.

    Article  CAS  Google Scholar 

  12. Trevino, M. B., and O’Conell, M. A. (1998) Plant Physiol., 116, 1461–1468.

    Article  PubMed  CAS  Google Scholar 

  13. Dani, V., Simon, W. J., Duranti, M., and Croy, R. R. (2005) Proteomics, 5, 737–745.

    Article  PubMed  CAS  Google Scholar 

  14. Kristensen, A. K., Brunstedt, J., Nielsen, K. K., Roepstorff, P., and Mikkelsen, J. D. (2000) Plant Sci., 155, 31–40.

    Article  PubMed  CAS  Google Scholar 

  15. Garcia-Olmedo, F., Molina, A., Segura, A., and Moreno, M. (1995) Trends Microbiol., 3, 72–74.

    Article  PubMed  CAS  Google Scholar 

  16. Blein, J., Thevenot, P., Marion, D., and Ponchet, M. (2002) Trends Plant Sci., 7, 293–296.

    Article  PubMed  CAS  Google Scholar 

  17. Park, S. Y., Jauh, G. Y., Mollet, J. C., Eckard, K. J., Nothnagel, E. A., Walling, L. L., and Lord, E. M. (2000) Plant Cell, 12, 151–163.

    Article  PubMed  CAS  Google Scholar 

  18. Eklund, D. M., and Edqvist, J. (2003) Plant Physiol., 132, 1249–1259.

    Article  PubMed  CAS  Google Scholar 

  19. Crimi, M., Astegno, A., Zoccatelli, G., and Esposti, M. D. (2006) Arch. Biochem. Biophys., 445, 65–71.

    Article  PubMed  CAS  Google Scholar 

  20. Douliez, J., Pato, C., Rabesona, H., Molle, D., and Marion, D. (2001) Eur. J. Biochem., 268, 1400–1403.

    Article  PubMed  CAS  Google Scholar 

  21. Douliez, J., Michon, T., and Marion, D. (2000) Biochim. Biophys. Acta, 1467, 65–72.

    Article  CAS  Google Scholar 

  22. Pato, C., Le Borgne, M., Le Baut, G., Le Pape, P., Marion, D., and Douliez, N. (2001) Biochem. Pharm., 62, 555–560.

    Article  PubMed  CAS  Google Scholar 

  23. Shewry, P. R., Beaudoin, F., Jenkins, J., Griffiths-Jones, S., and Mills, E. N. (2002) Biochem. Soc. Trans., 30, 906–910.

    Article  PubMed  CAS  Google Scholar 

  24. Diaz-Perales, A., Sanz, M. L., Garcia-Casado, G., Sanchez-Monge, R., Garcia-Selles, F. J., Lombardero, M., Polo, F., Gamboa, P. M., Barber, D., and Salcedo, G. (2003) J. Allergy Clin. Immunol., 111, 628–633.

    Article  PubMed  CAS  Google Scholar 

  25. Ballmer-Weber, B. K., Wangorsch, A., Bohle, B., Kaul, S., Kundig, T., Fotisch, K., van Ree, R., and Vieths, S. (2005) Clin. Exp. Allergy, 35, 970–978.

    Article  PubMed  CAS  Google Scholar 

  26. Schagger, H., and Jagow, G. (1987) Analyt. Biochem., 166, 368–379.

    Article  PubMed  CAS  Google Scholar 

  27. Gray, W. R. (1967) Meth. Enzymol., 11, 139–151.

    Article  CAS  Google Scholar 

  28. Ovchinnikova, T., Aleshina, G., Balandin, S., Krasnosdembskaya, A., Markelov, M., Frolova, E., Leonova, Y., Tagaev, A., Krasnodembsky, E., and Kokryakov, V. (2004) FEBS Lett., 577, 209–214.

    Article  PubMed  CAS  Google Scholar 

  29. Sambrook, J., Fritch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edn., Cold Springer Harbor Laboratory, NY.

    Google Scholar 

  30. Matz, M., Lukyanov, S., Bogdanova, E., Diatchenko, L., and Chenchik, A. (1999) Nucleic Acids Res., 27, 1558–1560.

    Article  PubMed  CAS  Google Scholar 

  31. Ostergaard, J., Hojrup, P., and Knudsen, J. (1995) Biochim. Biophys. Acta, 1254, 169–179.

    PubMed  Google Scholar 

  32. Soufleri, I. A., Vergnolle, C., Miginiac, E., and Kader, J. C. (1996) Planta, 199, 229–237.

    Article  PubMed  CAS  Google Scholar 

  33. Lullien-Pellerin, V., Devaux, C., Ihorai, T., Marion, D., Pahin, V., Joudrier, P., and Gautier, M.-F. (1999) Eur. J. Biochem., 260, 861–868.

    Article  PubMed  CAS  Google Scholar 

  34. Ge, X., Chen, J., Sun, C., and Cao, K. (2003) Prot. Eng., 16, 387–390.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997, Moscow, Russia

    E. I. Finkina, S. V. Balandin, N. A. Potapenko, A. A. Tagaev & T. V. Ovchinnikova

  2. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, ul. Pogodinskaya 10, 119121, Moscow, Russia

    M. V. Serebryakova

Authors
  1. E. I. Finkina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. V. Balandin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. V. Serebryakova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. A. Potapenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. A. Tagaev
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. T. V. Ovchinnikova
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Published in Russian in Biokhimiya, 2007, Vol. 72, No. 4, pp. 533–543.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM06-310, February 18, 2007.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Finkina, E.I., Balandin, S.V., Serebryakova, M.V. et al. Purification and primary structure of novel lipid transfer proteins from germinated lentil (Lens culinaris) seeds. Biochemistry Moscow 72, 430–438 (2007). https://doi.org/10.1134/S0006297907040104

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0006297907040104

Key words

Search

Navigation