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T-cadherin activates Rac1 and Cdc42 and changes endothelial permeability

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Abstract

In the present study, expression of T-cadherin was shown to induce intracellular signaling in NIH3T3 fibroblasts: it activated Rac1 and Cdc42 (p < 0.01) but not RhoA. T-Cadherin overexpression in human umbilical vein endothelial cells (HUVEC) using adenoviral constructs induced disassembly of microtubules and polymerization of actin stress fibers, whereas down-regulation of endogenous T-cadherin expression in HUVEC using lentiviral constructs resulted in micro-tubule polymerization and a decrease in the number of actin stress fibers. Moreover, suppression of the T-cadherin expression significantly decreased the endothelial monolayer permeability as compared to the control (p < 0.001).

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Abbreviations

α-GAPDH:

glyceraldehyde phosphate dehydrogenase

GFP:

green fluorescent protein

HUVEC:

human umbilical vein endothelial cells

shRNA:

short hairpin RNA

siRNA:

small interfering RNA

SRE:

serum response element

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Authors and Affiliations

  1. Institute of Experimental Cardiology, Cardiology Research Center, 3-ya Cherepkovskaya ul. 15a, 121552, Moscow, Russia

    E. V. Semina, P. N. Rutkevich & Y. V. Parfyonova

  2. Faculty of Fundamental Medicine, Lomonosov Moscow State University, Lomonosovskii pr. 31, Build. 5, 117192, Moscow, Russia

    K. A. Rubina & V. A. Tkachuk

  3. Department of Pharmacology, Illinois University, Chicago, USA

    T. A. Voyno-Yasenetskaya

Authors
  1. E. V. Semina
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  2. K. A. Rubina
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  3. P. N. Rutkevich
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  4. T. A. Voyno-Yasenetskaya
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  5. Y. V. Parfyonova
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  6. V. A. Tkachuk
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Corresponding author

Correspondence to K. A. Rubina.

Additional information

Original Russian Text © E. V. Semina, K. A. Rubina, P. N. Rutkevich, T. A. Voyno-Yasenetskaya, Y. V. Parfyonova, V. A. Tkachuk, 2009, published in Biokhimiya, 2009, Vol. 74, No. 4, pp. 448–458.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM08-318, February 15, 2009.

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Semina, E.V., Rubina, K.A., Rutkevich, P.N. et al. T-cadherin activates Rac1 and Cdc42 and changes endothelial permeability. Biochemistry Moscow 74, 362–370 (2009). https://doi.org/10.1134/S0006297909040026

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  • DOI: https://doi.org/10.1134/S0006297909040026

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