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In vitro assessment of thermal changes in human teeth during photodynamic antimicrobial chemotherapy performed with red light sources

  • Laser Methods in Chemistry, Biology, and Medicine
  • Published:
Laser Physics

Abstract

Abstract

Photo-disinfection of deep dentin caries lesions may be a useful tool for preserving tooth structure. However, increase of pulpal temperature higher than 5.5°C may put pulp vitality in danger.

Objectives

This in vitro study evaluated thermal effects of radiation of the red spectrum (λ = 600−700 nm, 94 J cm−2) on pulpal and periodontal areas when irradiating deep dentin cavities in human teeth.

Methods

Tooth preparation was performed at occlusal surface in 10 human teeth, which were irradiated by five different light units, four diode GaAlAs lasers: Coherent (C); Kondortech (B); Whitening lase (W); and K5000 with 2 different tips (K1 and K2), as well as one light emitting diode Laserbeam (LED). After application of toluidine blue O dye in the cavity, variation of temperature was measured in duplicate using a digital thermometer and 2 K-type thermocouples one placed inside the pulpal chamber and the other on root surface at the cementoenamel junction, simultaneously. The mean average temperature rise (ΔT) was calculated for each group.

Results

ΔT values were analyzed by ANOVA and Tukey tests. Pulpal temperature increase ranged from 0.62 ± 0.51 to 1.25 ± 0.50°C. Group C presented the highest ΔT, which was statistically significant different from B, K1, and K2. The lowest ΔT value was found for group K2, which statistically differed from the group W. No other statistically significant difference was found. At the root surface, ΔT values were lower than 0.5°C for all devices and no statistically significantly difference was found among them.

Conclusion

With regard to thermal side effects, the use of these red light sources operating at 94 J cm−2 may be considered harmless for dental tissues.

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Authors and Affiliations

  1. Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Rua Cap. Francisco Pedro S/N, 60430-170, Fortaleza, CE, Brazil

    D. M. de-Paula, M. A. S. Melo, J. P. M. Lima & L. K. A. Rodrigues

  2. Piracicaba Dental School, University of Campinas, Av. Limeira 901, 13414-903, Piracicaba, SP, Brazil

    M. Nobre-dos-Santos

  3. Faculty of Dentistry, Federal University of Ceará, Av. Geraldo Rangel 186, 62041-380, Sobral, CE, Brazil

    I. C. J. Zanin

Authors
  1. D. M. de-Paula
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  2. M. A. S. Melo
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  3. J. P. M. Lima
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  4. M. Nobre-dos-Santos
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  5. I. C. J. Zanin
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  6. L. K. A. Rodrigues
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Corresponding author

Correspondence to L. K. A. Rodrigues.

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Original Russian Text © Astro, Ltd., 2010.

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de-Paula, D.M., Melo, M.A.S., Lima, J.P.M. et al. In vitro assessment of thermal changes in human teeth during photodynamic antimicrobial chemotherapy performed with red light sources. Laser Phys. 20, 1475–1480 (2010). https://doi.org/10.1134/S1054660X10110046

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  • DOI: https://doi.org/10.1134/S1054660X10110046

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