Abstract
The genotoxicity of a proline-rich polypeptide was investigated in vitro in the human myeloid leukemia KCL-22 cell line. A comet assay, based on single-cell gel electrophoresis, was used for the analysis of the DNA damage and reparation rate. After electrophoresis, images resembling comets were formed; the intensity of the “tail” relative to the “head” reflected the number of DNA breaks. We demonstrated that the level of DNA damage depended on the polypeptide concentration and incubation time. Estimation of DNA damage levels by the comet assay is recommended for the evaluation of the genotoxicity of new pharmaceuticals.
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Galoyan, A.A., Proc. Int. Sci. Conf. Dedicated 75th Anniv. YSMU, Yerevan, 2005, pp. 135–136.
Brossier, F., Weber-Levy, M., Mock, M., and Sirard J.C., Infect. Immune, 2000, vol. 68, pp. 1781–1786.
Aprikyan, V.S. and Galoyan, A.A., Med. Sci. Armenia, 1999, vol. 31, no. 4, pp. 29–36.
Davtyan, T.K., Manukyan, N.A., Mkrtchyan, R., and Galoyan, A.A., Neurochem. Res., 2005, vol. 3, pp. 297–309.
Galoyan, A.A. and Aprikyan, V.S., Neurochem. Res., 2002, vol. 27, pp. 305–312.
Aprikyan, V.S. and Galoyan, A.A., Proc. Int. Conf. “Biochemical and Molecular-Biological Aspects of Brain Immune System”, Yerevan, 2001, pp. 183–189.
Aprikyan, V.S. and Galoyan, A.A., Neurokhimiya, 2000, vol. 17, pp. 60–63.
Galoyan, A.A., Brain Neurosecretory Cytokines; Immune Response and Neuronal Survival, New York: Kluwer Academic/Plenum Publishers, 2004.
Galoyan, A.A., Korochkin, L.I., Rybalkina, E.J., et al., Cell Transplantation, 2008.
Galoyan, A.A., Bezirganyan, K.B., and Davtyan, T.K., Cell Transplantation, 2008 (in press).
Olive, P.L., Meth. Mol. Biol., 2001, vol. 203, pp. 179–194.
Collins, A.R., Mol. Biotechnol., 2004, vol. 26, no. 3, pp. 249–261.
Ostling, O. and Johanson, K.J., Biochem. Biophys. Res. Commun., 1984, vol. 123, pp. 291–298.
Singh, N.P., McCoy, M.T., Tice, R.R., and Schneider, E.L., Experim. Cell Res., 1988, vol. 175, pp. 184–191.
Anderson, D., Yu, T.W., Philips, B.J., and Schmezer, P., Mutation Res., 1994, vol. 307, pp. 261–271.
Olive, P.L., Vlodek, D., and Banath, J.P., Cancer Res., 1991, vol. 51, pp. 4671–4676.
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Original Russian Text © A.A. Galoyan, K.S. Margaryan, G.G. Hovhannisyan, G.H. Gasparyan, D.N. Aroutiounian, R.M. Aroutiounian, 2009, published in Neirokhimiya, 2009, Vol. 26, No. 2, pp. 156–160.
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Galoyan, A.A., Margaryan, K.S., Hovhannisyan, G.G. et al. Study of the genotoxic effects of a proline-rich polypeptide using the comet assay. Neurochem. J. 3, 145–148 (2009). https://doi.org/10.1134/S1819712409020111
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DOI: https://doi.org/10.1134/S1819712409020111