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Disordered Meiotic Regulation of Oocytes by Duration of Diabetes Mellitus in BBdp Rat

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Abstract

Diabetes mellitus (DM) disturbs normal functions from the level of cells to the level of organs. In this study, the authors explore the detrimental effects of type 1 diabetes on meiotic regulation depending on the duration of DM. In non—diabetes-prone BioBreeding (BBdr) control rats, most of the large follicles had germinal vesicle (GV)—intact oocytes. Conversely, a decrease of intact GV that was dependent on the duration of diabetic symptoms was observed; only 54% of the large follicles of diabetes-prone BB (BBdp) rats had GV-intact oocytes at 6 weeks after diabetes induction. Furthermore, some of the secondary follicles in BBdp rats also had germinal vesicle breakdown (GVB) oocytes. The nuclear status of the euglycemia BBdp rat was similar to those of the BBdr rat. In BBdp rats, the rate of meiotic progression to the metaphase II stage was significantly lower; however, the rate of segregated oocytes was significantly increased compared with controls during induction of in vitro maturation. The rate of segregated oocytes was not affected by the presence of the cumulus after chronic symptoms. These results indicate that chronic DM has a detrimental effect on meiotic regulation during folliculogenesis and results in a reduced number of competent oocytes. In addition, these data suggest that the follicle cells can resume supporting the meiotic regulation under euglycemia through insulin administration, independent of the duration of DM.

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Authors and Affiliations

  1. College of Veterinary Medicine, Kyungpook National University, Daegu, Korea

    KilSoo Kim DVM, PhD

  2. Department of Obstetrics/Gynecology, College of Medicine, Ulsan University, Seoul, Korea

    Chung Hoon Kim MD, PhD

  3. Department of Obstetrics/Gynecology and Cell Biology and Physiology, School of Medicine, Washington University, St Louis, Missouri, USA

    Kelle H. Moley MD, PhD

  4. Department of Biology, Institute of Basic Sciences, College of Natural Sciences, Sungshin Women’s University, Seoul, 136-742, Korea

    Yong-Pil Cheon PhD

Authors
  1. KilSoo Kim DVM, PhD
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  2. Chung Hoon Kim MD, PhD
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  3. Kelle H. Moley MD, PhD
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  4. Yong-Pil Cheon PhD
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Corresponding author

Correspondence to Yong-Pil Cheon PhD.

Additional information

This work was supported by the Sungshin Women’s University Research grant of 2006.

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Kim, K., Kim, C.H., Moley, K.H. et al. Disordered Meiotic Regulation of Oocytes by Duration of Diabetes Mellitus in BBdp Rat. Reprod. Sci. 14, 467–474 (2007). https://doi.org/10.1177/1933719107306228

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