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Isolation and Characterization of Side Population Cells in the Postpartum Murine Endometrium

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Abstract

Endometrium is a highly active organ that is periodically remodeled during the life span. Previous studies have indicated the presence of an adult stem or progenitor cell population in this tissue. In this study, side population (SP) cells were isolated from the endometrium of postpartum murine uterus but not from the endometrium of a uterus undergoing a normal estrus cycle. Phenotype analysis showed that SP cells were negative for hematopoietic, endothelial, and mesenchymal stem cell (MSC) markers, but they expressed stem cell antigen 1 (Sca-1) and c-kit at various degrees. Side population cell is a heterogeneous population of endometrial stem/progenitor cells that have colony-forming capacity. They were found to reside in quiescence in the stroma but not in the luminal epithelium. These data suggest that, like other tissues and organs, the murine endometrium also contains SP cells. Their specific role in the regeneration of the endometrium warrants further study.

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Authors and Affiliations

  1. Jiangsu Province Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China

    Fei-Fei Hu PhD candidate, Yu-Gui Cui PhD & Jia-Yin Liu MD, PhD

  2. Centre of Clinical Reproductive Medicine, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu Province, 210029, China

    Fei-Fei Hu PhD candidate, Jing Xu Master Degree candidate, Yu-Gui Cui PhD, Xiao-Qiao Qian PhD, Yun-Dong Mao PhD & Jia-Yin Liu MD, PhD

  3. Stem Cell Laboratory, Fujian Second People’s Hospital, Fuzhou, China

    Lian-Ming Liao PhD

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  1. Fei-Fei Hu PhD candidate
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Correspondence to Jia-Yin Liu MD, PhD.

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Hu, FF., Xu, J., Cui, YG. et al. Isolation and Characterization of Side Population Cells in the Postpartum Murine Endometrium. Reprod. Sci. 17, 629–642 (2010). https://doi.org/10.1177/1933719110369180

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  • DOI: https://doi.org/10.1177/1933719110369180

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