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Effect of Exogenous Anti-Müllerian Hormone Treatment on Cryopreserved and Transplanted Mouse Ovaries

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Abstract

Follicle loss occurs after ovary cryopreservation and transplantation. To preserve the follicle pool of cryopreserved or grafted ovaries, anti-Müllerian hormone (AMH), which inhibits ovarian follicle recruitment, was used in a mouse model. In experiment 1, ovaries were vitrified warmed with different doses of AMH (0, 5, 15, or 45 μg/mL) supplementation. In experiment 2, AMH (0, 50, 250, and 1250 μg/mL) was injected into mice before and/or after cryopreserved ovary autotransplantation, and the recipients remained for 7 or 28 days after grafting. Ovaries were evaluated by follicle morphology, density, and apoptosis ratio. Additionally, serum follicle-stimulating hormone was measured in experiment 2. Significantly decreased follicle apoptosis were detected in AMH-treated groups when compared to the control ovaries in experiment 1, meanwhile no positive effect of exogenous AMH was found in experiment 2. Thus, we suggest AMH supplementation during ovary vitrification warming has beneficial effect on reducing follicle apoptosis.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, 166 Gumi-ro, 463-707, Seongnam, Geonggi-do, Korea

    Hyun Sun Kong MS, Seul Ki Kim MD, Jaewang Lee MS, Hye Won Youm PhD, Jung Ryeol Lee MD, PhD & Chang Suk Suh MD, PhD

  2. Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea

    Hyun Sun Kong MS, Jaewang Lee MS, Jung Ryeol Lee MD, PhD, Chang Suk Suh MD, PhD & Seok Hyun Kim MD, PhD

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  1. Hyun Sun Kong MS
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  2. Seul Ki Kim MD
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Correspondence to Jung Ryeol Lee MD, PhD.

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Kong, H.S., Kim, S.K., Lee, J. et al. Effect of Exogenous Anti-Müllerian Hormone Treatment on Cryopreserved and Transplanted Mouse Ovaries. Reprod. Sci. 23, 51–60 (2016). https://doi.org/10.1177/1933719115594021

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  • DOI: https://doi.org/10.1177/1933719115594021

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