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Rapid Screening and Identification of Polar Constituents from Brazilian Arnica Lychnophora sp. by LC-UV/DAD-ESI-MS and LC-UV/DAD-ESI-MS/MS Analysis

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Abstract

This paper describes an analytical method for the rapid screening and identification of the phenolic constituents present in the polar extracts of different Lychnophora spp. using LC-UV/DAD-ESI-MS and LC-UV/DAD-ESI-MS/MS. Compounds were identified based on UV, retention time, MS experiments and MS/MS of precursor ion or standard. On-line phytochemical investigation of Lychnophora spp. allowed for the identification of flavonoids, chlorogenic acid derivatives and lactones. Some of the observed compounds were for the first time identified in Lychnophora species in a fast analytical procedure. The data obtained here may be helpful to the investigation of polar constituents from other Lychnophora species.

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Acknowledgments

The authors wish to acknowledge the Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq (research fellowship to N·P.L.) and the Fundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP (fellowship to S.L.M., 05/53877-0) of Brazil for financial support (2003/06328-6 and 03/02176-7R).

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Authors and Affiliations

  1. Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café s/n◦, Ribeirão Preto, São Paulo, SP, CEP 14040-903, Brazil

    Solange Leite de Moraes, José Carlos Tomaz & Norberto Peporine Lopes

  2. Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, SP, CEP 14040-901, Brazil

    Luiz Elídio Gregório

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  1. Solange Leite de Moraes
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  2. Luiz Elídio Gregório
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  3. José Carlos Tomaz
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  4. Norberto Peporine Lopes
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Corresponding author

Correspondence to Norberto Peporine Lopes.

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Separation Analysis Applied to Pharmaceutical Sciences in Brazil.

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de Moraes, S.L., Gregório, L.E., Tomaz, J.C. et al. Rapid Screening and Identification of Polar Constituents from Brazilian Arnica Lychnophora sp. by LC-UV/DAD-ESI-MS and LC-UV/DAD-ESI-MS/MS Analysis. Chroma 69 (Suppl 2), 157–165 (2009). https://doi.org/10.1365/s10337-009-1036-9

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