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LC with Column-Switching and Spectrophotometric Detection for Determination of Risperidone and 9-Hydroxyrisperidone in Human Serum

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Abstract

An automated high performance liquid chromatography with column-switching and ultraviolet detection was developed for the analysis of risperidone and 9-hydroxyrisperidone. The method needs minimum sample preparation and is useful for the detection down to a limit of 1 ng mL−1. Sample clean-up of serum was carried out on a CN 20 μm SPE-column using 8% (v/v) acetonitrile in water. Chromatographic separation was performed on ODS Hypersil C18 material with 38% (v/v) acetonitrile and 0.4% (v/v) TEMED in water. Application of the method to the analysis of serum samples confirmed its suitability for therapeutic drug monitoring of risperidone and 9-hydroxyrisperidone.

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Acknowledgments

We thank Sandra Heller for skilful technical assistance and Janssen–Cilag (Beerse, Belgium) for kindly supplying us with 9-hydroxyrisperidone pure drug substance.

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Authors and Affiliations

  1. Department of Psychiatry, University of Mainz, 55131, Mainz, Germany

    Katrin M. Kirschbaum, Susann Finger, Friederike Vogel & Christoph Hiemke

  2. Department of Psychiatry, University of Würzburg, 97080, Würzburg, Germany

    Rainer Burger & Peter Riederer

  3. Department of Child and Adolescent Psychiatry, University of Würzburg, 97080, Würzburg, Germany

    Manfred Gerlach

Authors
  1. Katrin M. Kirschbaum
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  2. Susann Finger
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  3. Friederike Vogel
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  4. Rainer Burger
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  5. Manfred Gerlach
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  6. Peter Riederer
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  7. Christoph Hiemke
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Correspondence to Katrin M. Kirschbaum.

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Kirschbaum, K.M., Finger, S., Vogel, F. et al. LC with Column-Switching and Spectrophotometric Detection for Determination of Risperidone and 9-Hydroxyrisperidone in Human Serum. Chroma 67, 321–324 (2008). https://doi.org/10.1365/s10337-007-0506-1

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  • DOI: https://doi.org/10.1365/s10337-007-0506-1

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