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Simple In Vitro Cultivation of the Malaria Parasite Plasmodium falciparum (Erythrocytic Stages)

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Protocols in Molecular Parasitology

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 21))

Abstract

Malaria represents the world’s greatest public health problem in terms of number of people affected, and the levels of morbidity and mortality. The protozoan malaria parasites (Plasmodium spp.) are transmitted by infected female mosquitoes when feeding on blood. Parasites soon enter liver cells, and after several days of multiplication, are released into the bloodstream where further cycles of asexual reproduction occur, giving rise to the clinical symptoms of malaria. Some erythrocytic parasites will differentiate into presexual forms (gametocytes; see Chapter 6), which when taken up by mosquitoes in further blood meals, mature into gametes and undergo a sexual cycle. With the eventual release of infective sporozoites into the mosquito salivary glands, the life cycle of the parasite is completed.

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Further Reading

  • Bruce-Chwatt, L. J. (1985) Essential Malariology. William Heinemann Medical Books, London.

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  • Hyde, J. E. ( 1990) Molecular Parasitology. Open University Press/John Wiley, Chichester, UK, and Van Nostrand Reinhold, New York

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Technology (UMIST), Manchester, UK

    Martin Read

  2. University of Manchester Institute of Science and Technology, Manchester, UK

    John E. Hyde

Authors
  1. Martin Read
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  2. John E. Hyde
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Editor information

Editors and Affiliations

  1. University of Manchester Institute of Science and Technology, Manchester, UK

    John E. Hyde

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© 1993 Humana Press Inc, Totowa, NJ

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Read, M., Hyde, J.E. (1993). Simple In Vitro Cultivation of the Malaria Parasite Plasmodium falciparum (Erythrocytic Stages). In: Hyde, J.E. (eds) Protocols in Molecular Parasitology. Methods in Molecular Biology™, vol 21. Humana Press. https://doi.org/10.1385/0-89603-239-6:43

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  • DOI: https://doi.org/10.1385/0-89603-239-6:43

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-239-2

  • Online ISBN: 978-1-59259-508-2

  • eBook Packages: Springer Protocols

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