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Angiogenesis Assays Using Chick Chorioallantoic Membrane

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Angiogenesis Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 46))

Abstract

The study of the angiogenic process and the search for novel therapeutic agents to inhibit, or stimulate, angiogenesis has employed a wide range of in vivo ’angiogenesis’ assays (reviewed in 1–3). These differ greatly in their difficulty, quantitative nature, rapidity, and cost. The classical in vivo models include the rabbit ear chamber, hamster cheek pouch, dorsal skin chamber, dorsal skin and air-sac model, anterior chamber/iris and avascular corneal pocket assay, and the chick embryo chorioallantoic membrane (CAM) assay. More recent methods involve the implantation of preloaded Matrigel or alginate plugs, or collagen or poly vinyl sponges (1). Largely owing to its simplicity and low cost, the CAM is the most widely used in vivo model for the study of both angiogenesis and antiangiogenesis (1,4)

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Author information

Authors and Affiliations

  1. Department of Immunology,Faculty of Medicine, University of Liverpool, Liverpool, UK

    David C. West

  2. Department of Pathology, Medical School, Aberdeen Royal Infirmary, Aberdeen, UK

    W. Douglas Thompson

  3. Alistair Reid Venom Research Unit, Liverpool School of Tropical Medicine, Liverpool, UK

    Paula G. Sells

  4. Experimental Oncology Division, Institut de Rechèrches Servier, Suresnes, France

    Mike F. Burbridge

Authors
  1. David C. West
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  2. W. Douglas Thompson
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  3. Paula G. Sells
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  4. Mike F. Burbridge
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Editor information

Editors and Affiliations

  1. University of Nottingham and City Hospital, Nottingham, UK

    J. Clifford Murray

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© 2001 Humana Press Inc.

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West, D.C., Thompson, W.D., Sells, P.G., Burbridge, M.F. (2001). Angiogenesis Assays Using Chick Chorioallantoic Membrane. In: Murray, J.C. (eds) Angiogenesis Protocols. Methods in Molecular Medicine™, vol 46. Humana Press. https://doi.org/10.1385/1-59259-143-4:107

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  • DOI: https://doi.org/10.1385/1-59259-143-4:107

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-698-7

  • Online ISBN: 978-1-59259-143-5

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