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Structural differences between light and heavy rare earth elment binding chlorophylls in naturally grown fern Dicranopteris linearis

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Abstract

Chloroplasts and chlorophylls were isolated from the leaves of Dicranopteris linearis, a natural perennial fern sampled at rare earth element (REE) mining areas in the South-Jiangxi region (southern China). The inductively coupled plasma-mass spectrometry (ICP-MS) results indicated that REEs were present in the chloroplasts and chlorophylls of D. linearis. The in vivo coordination environment of light REE (lanthanum) or heavy REE (yttrium) ions in D. linearis chlorophyll-a was determind by the extended X-ray absorption fine structure (EXAFS). Results revealed that there were eight nitrogen atoms in the first coordination shell of the lanthanum atom, whereas there were four nitrogen atoms in the first coordination shell of yttrium. It was postulated that the lanthanum-chlorophyll-a complex might have a double-layer sandwichlike structure, but yttrium-binding chlorophyll-a might be in a single-layer form. Because the content of REE-binding chlorophylls in D. linearis chlorophylls was very low, it is impossible to obtain structural characteristics of REE-binding chlorophylls by direct analysis of the Fourier transform infrared (FTIR) and ultraviolet (UV)-visible spectra of D. linearis chlorophylls. In order to acquire more structural information of REE-binding chlorophyll-a in D. linearis, lanthanum—and yttrium-chlorophyll-a complexes were in vitro synthesized in acetone solution. Element analyses and EXAFS results indicated that REE ions (lanthanum or yttrium) of REE-chlorophyll-a possessed the same coordination environment whether in vivo or in vitro. The FTIR spectra of the REE-chlorophyll-a complexes indicated that REEs were bound to the porphyrin rings of chlorophylls. UV-visible results showed that the intensity ratios of Soret to the Q-band of REE-chlorophyll-a complexes were higher than those of standard chlorophyll-a and pheophytin-a indicating that REE-chlorophyll-a might have a much stronger ability to absorb the ultraviolet light. The MCD spectrum in the Soret band region of lanthanum-chlorophyll-a showed a special peak, but yttrium-chlorophyll-a did not have this special peak, corresponding well to their double-layer and single-layer structure, respectively. Structural differences between lanthanum—and yttrium-chlorophyll-a might result from the difference in ion radius between yttrium and lanthanum. These data might be useful for understanding of both the properties of REE-chlorophyll-a complexes and the physiological roles of REEs in the hyperaccumulator D. linearis.

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Authors and Affiliations

  1. College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China

    Zhenggui Wei, Huixin Li & Feng Hu

  2. Department of Biology, College of Life Sciences, Suzhou University, 215006, Suzhou, People's Republic of China

    Fashui Hong

  3. National Research Center for Geonalysis, Chinese Academy of Geological Sciences, 10037, Beijing, People's Republic of China

    Ming Yin

  4. Department of Chemistry, University of Science and Technology of China, 230026, Hefei, People's Republic of China

    Guiwen Zhao

  5. Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, People's Republic of China

    Jonathan Woonchung Wong

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Wei, Z., Hong, F., Yin, M. et al. Structural differences between light and heavy rare earth elment binding chlorophylls in naturally grown fern Dicranopteris linearis . Biol Trace Elem Res 106, 279–297 (2005). https://doi.org/10.1385/BTER:106:3:279

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  • DOI: https://doi.org/10.1385/BTER:106:3:279

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