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Universal primers for the PCR-mediated amplification of DNA 1: A satellite-like molecule associated with begomovirus-DNA β complexes

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Abstract

DNA 1 is a single-stranded DNA molecule of approximately 1370 nucleotides. It is associated with monopartite geminiviruses of the genus Begomovirus, which require a DNA β component for symptomatic infection. The DNA 1 molecule requires the helper begomovirus for movement in plants, but is capable of self-replication. We designed two abutting primer pairs (DNA101/DNA102 and UN101/UN102) to conserved sequences of DNA 1. This allowed polymerase chain reaction-mediated amplification of the full-length molecule from total nucleic acid extracts produced from various host plants from geographically distinct, worldwide locations. These primers are useful both as diagnostic probes and for producing full-length infectious clones for in planta studies.

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Authors and Affiliations

  1. Department of Disease and Stress Biology, John Innes Centre, Colney Lane, NR4 7UH, Norwich, UK

    S. E. Bull

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  1. S. E. Bull
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  2. R. W. Briddon
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  3. P. G. Markham
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Correspondence to S. E. Bull.

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Bull, S.E., Briddon, R.W. & Markham, P.G. Universal primers for the PCR-mediated amplification of DNA 1: A satellite-like molecule associated with begomovirus-DNA β complexes. Mol Biotechnol 23, 83–86 (2003). https://doi.org/10.1385/MB:23:1:83

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