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Comparative proteome analysis of breast cancer and normal breast

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Abstract

Breast cancer is a leading cause of death for women. The underlying molecular mechanism is still not well understood. In this study, two-dimensional gel electrophoresis combined with mass spectrometry was used to analyze changes in the proteome of infiltrating ductal carcinoma compared to normal breast tissue. Ten sets of two-dimensional gels per experimental condition were analyzed and more than 500 spots each were detected. This revealed 39 spots for which expression in breast cancer cells were reproducibly altered more than twofold compared to normal controls (p<0.01). These spots represented 25 different proteins after identification using the database search after mass spectrometry, comprising cell defense proteins, enzymes involved in glycolytic energy metabolism and homeostasis, protein folding and structural proteins, proteins involved in cytoskeleton and cell motility, and proteins involved in other functions. In addition, 28 nondifferentially expressed proteins with different functions were also mapped and identified, which might help to establish a two-dimensional gel electrophoresis reference map of human breast cancer. Our study shows that proteomics offers a powerful methodology to detect the proteins that show different expression patterns in breast cancer tissue and may provide an accurate molecular classification. The differentially expressed proteins may be used as potential candidate markers for diagnostic purposes or for determination of tumor sensitivity to therapy. The functional implications of the identified proteins are discussed.

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Authors and Affiliations

  1. National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, 5 Dong Dan San Tiao, 100005, Beijing, China

    Yuanming Luo, Jindan Zhang, Yanxin Liu & Dexian Zheng

  2. Proteomics Research Center of Chinese Academy of Medical Sciences, 100176, Beijing, China

    Yuanming Luo, Xiaorong Wang, Shuzhen Wu, Youhe Gao & Dexian Zheng

  3. Novo Nordisk R & D Center China, 100176, Beijing, China

    Allan Christian Shaw

  4. Peking Union Medical College Hospital, 100730, Beijing, China

    Xuan Zeng & Jie Chen

Authors
  1. Yuanming Luo
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  2. Jindan Zhang
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  3. Yanxin Liu
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  4. Allan Christian Shaw
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  5. Xiaorong Wang
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  6. Shuzhen Wu
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  7. Xuan Zeng
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  8. Jie Chen
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  10. Dexian Zheng
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Correspondence to Dexian Zheng.

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Luo, Y., Zhang, J., Liu, Y. et al. Comparative proteome analysis of breast cancer and normal breast. Mol Biotechnol 29, 233–244 (2005). https://doi.org/10.1385/MB:29:3:233

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